Details
| Original language | English |
|---|---|
| Pages (from-to) | 2243-2245 |
| Number of pages | 3 |
| Journal | Angewandte Chemie - International Edition |
| Volume | 38 |
| Issue number | 15 |
| Publication status | Published - 28 Jul 1999 |
Abstract
Covalent linkage of the arginine-rich fragment of the Tat protein to 1,4,7,10-tetraazacyclododecane (cyclen) results in the selective cleavage of the TAR-RNA of HIV-1 (see picture; the biotin at the 5' end acts as a label for the subsequent analysis of the cleavage fragments). The cleavage occurs at room temperature and is diminished when Eu(III) ions are present - at a concentration of about 1/10 of the concentration of the peptide-cyclen conjugate. The pH dependence indicates that two ammonium ions are responsible for the cleavage reaction. The white arrows in the schematic diagram mark the cleavage sites in RNaseT1, and the black arrows the sites in the peptide- cyclen conjugate.
Keywords
- Macrocycles, Nucleic acids, Peptides, RNA
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Chemistry(all)
Sustainable Development Goals
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In: Angewandte Chemie - International Edition, Vol. 38, No. 15, 28.07.1999, p. 2243-2245.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Selective cleavage of the HIV-1 TAR-RNA with a peptide-cyclen conjugate
AU - Michaelis, Katrin
AU - Kalesse, Markus
PY - 1999/7/28
Y1 - 1999/7/28
N2 - Covalent linkage of the arginine-rich fragment of the Tat protein to 1,4,7,10-tetraazacyclododecane (cyclen) results in the selective cleavage of the TAR-RNA of HIV-1 (see picture; the biotin at the 5' end acts as a label for the subsequent analysis of the cleavage fragments). The cleavage occurs at room temperature and is diminished when Eu(III) ions are present - at a concentration of about 1/10 of the concentration of the peptide-cyclen conjugate. The pH dependence indicates that two ammonium ions are responsible for the cleavage reaction. The white arrows in the schematic diagram mark the cleavage sites in RNaseT1, and the black arrows the sites in the peptide- cyclen conjugate.
AB - Covalent linkage of the arginine-rich fragment of the Tat protein to 1,4,7,10-tetraazacyclododecane (cyclen) results in the selective cleavage of the TAR-RNA of HIV-1 (see picture; the biotin at the 5' end acts as a label for the subsequent analysis of the cleavage fragments). The cleavage occurs at room temperature and is diminished when Eu(III) ions are present - at a concentration of about 1/10 of the concentration of the peptide-cyclen conjugate. The pH dependence indicates that two ammonium ions are responsible for the cleavage reaction. The white arrows in the schematic diagram mark the cleavage sites in RNaseT1, and the black arrows the sites in the peptide- cyclen conjugate.
KW - Macrocycles
KW - Nucleic acids
KW - Peptides
KW - RNA
UR - http://www.scopus.com/inward/record.url?scp=0033517092&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1521-3773(19990802)38:15<2243::AID-ANIE2243>3.0.CO;2-V
DO - 10.1002/(SICI)1521-3773(19990802)38:15<2243::AID-ANIE2243>3.0.CO;2-V
M3 - Article
AN - SCOPUS:0033517092
VL - 38
SP - 2243
EP - 2245
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 15
ER -