Details
| Original language | English |
|---|---|
| Pages (from-to) | 732-743 |
| Number of pages | 12 |
| Journal | Molecular Nutrition and Food Research |
| Volume | 49 |
| Issue number | 8 |
| Publication status | Published - 27 Jul 2005 |
Abstract
The industrial utilisation of the metabolic capacities of cells depends on the successful scaling-up of laboratory trials. The considerations usually start with the preparation of chemical elemental balances, or by monitoring and calculating the kinetics of growth. Input of mechanical energy and transfer of heat and of nutrients including oxygen are key to the process and differ among reactor types. The production of the character impact flavour compound 4-decanolide by the basidiomycetous fungus Tyromyces sambuceus in a fed- batch stirred tank process is presented as a practical example of the scale-up of the production, of a small, bioactive molecule. The scaling-up of a downstreaming process including some cost estimations is demonstrated using lactoferrin, a bioactive protein from whey. The concerted scale-up of a novel bioprocess is facilitated today by advanced bioreactor designs and downstreaming equipment, and by a set of proven strategies. However, specific properties of the microorganism, of the nutrient medium used, or of the product aimed at may rise unexpected problems. These can ultimately be tackled by experiments only.
Keywords
- 4-Decanolide, Bioreactor, Downstreaming, Lactoferrin, Mass balances, Membrane adsorber, Scale-up calculations, Similarity theory
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Food Science
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In: Molecular Nutrition and Food Research, Vol. 49, No. 8, 27.07.2005, p. 732-743.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Scale-up of natural product formation and isolation
AU - Berger, Ralf G.
AU - Scheper, Thomas
AU - Schügerl, Karl
PY - 2005/7/27
Y1 - 2005/7/27
N2 - The industrial utilisation of the metabolic capacities of cells depends on the successful scaling-up of laboratory trials. The considerations usually start with the preparation of chemical elemental balances, or by monitoring and calculating the kinetics of growth. Input of mechanical energy and transfer of heat and of nutrients including oxygen are key to the process and differ among reactor types. The production of the character impact flavour compound 4-decanolide by the basidiomycetous fungus Tyromyces sambuceus in a fed- batch stirred tank process is presented as a practical example of the scale-up of the production, of a small, bioactive molecule. The scaling-up of a downstreaming process including some cost estimations is demonstrated using lactoferrin, a bioactive protein from whey. The concerted scale-up of a novel bioprocess is facilitated today by advanced bioreactor designs and downstreaming equipment, and by a set of proven strategies. However, specific properties of the microorganism, of the nutrient medium used, or of the product aimed at may rise unexpected problems. These can ultimately be tackled by experiments only.
AB - The industrial utilisation of the metabolic capacities of cells depends on the successful scaling-up of laboratory trials. The considerations usually start with the preparation of chemical elemental balances, or by monitoring and calculating the kinetics of growth. Input of mechanical energy and transfer of heat and of nutrients including oxygen are key to the process and differ among reactor types. The production of the character impact flavour compound 4-decanolide by the basidiomycetous fungus Tyromyces sambuceus in a fed- batch stirred tank process is presented as a practical example of the scale-up of the production, of a small, bioactive molecule. The scaling-up of a downstreaming process including some cost estimations is demonstrated using lactoferrin, a bioactive protein from whey. The concerted scale-up of a novel bioprocess is facilitated today by advanced bioreactor designs and downstreaming equipment, and by a set of proven strategies. However, specific properties of the microorganism, of the nutrient medium used, or of the product aimed at may rise unexpected problems. These can ultimately be tackled by experiments only.
KW - 4-Decanolide
KW - Bioreactor
KW - Downstreaming
KW - Lactoferrin
KW - Mass balances
KW - Membrane adsorber
KW - Scale-up calculations
KW - Similarity theory
UR - http://www.scopus.com/inward/record.url?scp=23944493570&partnerID=8YFLogxK
U2 - 10.1002/mnfr.200400103
DO - 10.1002/mnfr.200400103
M3 - Review article
C2 - 15991214
AN - SCOPUS:23944493570
VL - 49
SP - 732
EP - 743
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
SN - 1613-4125
IS - 8
ER -