Details
| Original language | English |
|---|---|
| Pages (from-to) | 354-358 |
| Number of pages | 5 |
| Journal | Journal of Applied Botany and Food Quality |
| Volume | 90 |
| Publication status | Published - 1 Jan 2017 |
Abstract
There is a great demand of salt-tolerant sugarcane planting material in Cuba. Temporary immersion bioreactors (TIB) are effective to significantly increase sugarcane in vitro shoot proliferation rate from 1:4 in conventional containers to about 1:35. Sugarcane micropropagation in TIBs under NaCl stress may help screen mutants with salinity tolerance. We developed the experiment shown here to identify a NaCl concentration able to stress shoot in TIBs. At 30 days of culture initiation with different NaCl levels (0 - 200 mM), explant multiplication rate, shoot cluster fresh mass, and levels of aldehydes, chlorophylls, carotenoids and phenolics were determined in the plant material. Content of soluble phenolics in the culture medium was also evaluated. Addition of NaCl decreased shoot multiplication rate and fresh mass. Other statistically significant differences were recorded but the most important were noted in the increased contents of carotenoids, malondialdehyde, other aldehydes and soluble phenolics in the plants, and in the soluble phenolics in the culture medium. This research may be useful for future experiments of in vitro selection of new sugarcane genetic materials with NaCl tolerance. Fifty percent of multiplication rate was reduced with 89 mM NaCl which can be used to stress shoots during micropropagation in TIBs and eventually detect mutants with salt tolerance.
Keywords
- In vitro salt stress, In vitro selection, Plant metabolites, Saccharum sp., Salinity
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of Applied Botany and Food Quality, Vol. 90, 01.01.2017, p. 354-358.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Salinity induces specific metabolic changes in sugarcane shoot explants in temporary immersion bioreactors
AU - Gómez, Daviel
AU - Hernández, Lázaro
AU - Valle, Bárbara
AU - Martínez, Julia
AU - Cid, Mariela
AU - Escalona, Maritza
AU - Hernández, Martha
AU - Yabor, Lourdes
AU - Beemster, Gerrit T.S.
AU - Tebbe, Christoph C.
AU - Papenbrock, Jutta
AU - Lorenzo, José Carlos
N1 - Funding information: This research was supported by the Institute of Botany (Leibniz University of Hannover, Germany), the Laboratory for Integrated Plant Physiology Research (University of Antwerp, Belgium), the Thünen Institute of Biodiversity (Braunschweig, Germany) and the Bioplant Centre (University of Ciego de Ávila, Cuba).
PY - 2017/1/1
Y1 - 2017/1/1
N2 - There is a great demand of salt-tolerant sugarcane planting material in Cuba. Temporary immersion bioreactors (TIB) are effective to significantly increase sugarcane in vitro shoot proliferation rate from 1:4 in conventional containers to about 1:35. Sugarcane micropropagation in TIBs under NaCl stress may help screen mutants with salinity tolerance. We developed the experiment shown here to identify a NaCl concentration able to stress shoot in TIBs. At 30 days of culture initiation with different NaCl levels (0 - 200 mM), explant multiplication rate, shoot cluster fresh mass, and levels of aldehydes, chlorophylls, carotenoids and phenolics were determined in the plant material. Content of soluble phenolics in the culture medium was also evaluated. Addition of NaCl decreased shoot multiplication rate and fresh mass. Other statistically significant differences were recorded but the most important were noted in the increased contents of carotenoids, malondialdehyde, other aldehydes and soluble phenolics in the plants, and in the soluble phenolics in the culture medium. This research may be useful for future experiments of in vitro selection of new sugarcane genetic materials with NaCl tolerance. Fifty percent of multiplication rate was reduced with 89 mM NaCl which can be used to stress shoots during micropropagation in TIBs and eventually detect mutants with salt tolerance.
AB - There is a great demand of salt-tolerant sugarcane planting material in Cuba. Temporary immersion bioreactors (TIB) are effective to significantly increase sugarcane in vitro shoot proliferation rate from 1:4 in conventional containers to about 1:35. Sugarcane micropropagation in TIBs under NaCl stress may help screen mutants with salinity tolerance. We developed the experiment shown here to identify a NaCl concentration able to stress shoot in TIBs. At 30 days of culture initiation with different NaCl levels (0 - 200 mM), explant multiplication rate, shoot cluster fresh mass, and levels of aldehydes, chlorophylls, carotenoids and phenolics were determined in the plant material. Content of soluble phenolics in the culture medium was also evaluated. Addition of NaCl decreased shoot multiplication rate and fresh mass. Other statistically significant differences were recorded but the most important were noted in the increased contents of carotenoids, malondialdehyde, other aldehydes and soluble phenolics in the plants, and in the soluble phenolics in the culture medium. This research may be useful for future experiments of in vitro selection of new sugarcane genetic materials with NaCl tolerance. Fifty percent of multiplication rate was reduced with 89 mM NaCl which can be used to stress shoots during micropropagation in TIBs and eventually detect mutants with salt tolerance.
KW - In vitro salt stress
KW - In vitro selection
KW - Plant metabolites
KW - Saccharum sp.
KW - Salinity
UR - http://www.scopus.com/inward/record.url?scp=85044638041&partnerID=8YFLogxK
U2 - 10.5073/JABFQ.2017.090.044
DO - 10.5073/JABFQ.2017.090.044
M3 - Article
AN - SCOPUS:85044638041
VL - 90
SP - 354
EP - 358
JO - Journal of Applied Botany and Food Quality
JF - Journal of Applied Botany and Food Quality
SN - 1613-9216
ER -