Details
| Original language | English |
|---|---|
| Pages (from-to) | 713-720 |
| Number of pages | 8 |
| Journal | Chemistry and Biology |
| Volume | 9 |
| Issue number | 6 |
| Early online date | 16 Jun 2002 |
| Publication status | Published - Jun 2002 |
| Externally published | Yes |
Abstract
New insight into the importance of carbohydrates in biological systems underscores the need for rapid synthetic and screening procedures for them. Development of an organic synthesis-compatible linker that would attach saccharides to microtiter plates was therefore undertaken to facilitate research in glycobiology. Galactosyllipids containing small, hydrophobic groups at the anomeric position were screened for noncovalent binding to microtiter plates. When the lipid component was a saturated hydrocarbon between 13 and 15 carbons in length, the monosaccharide showed complete retention after aqueous washing and could be utilized in biological assays. This alkyl chain was also successfully employed with more complex oligosaccharides in biological assays. In light of these findings, this method of attachment of oligosaccharides to microtiter plates should be highly efficacious to high-throughput synthesis and analyses of carbohydrates in biological assays.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmacology
- Pharmacology, Toxicology and Pharmaceutics(all)
- Drug Discovery
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
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In: Chemistry and Biology, Vol. 9, No. 6, 06.2002, p. 713-720.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Saccharide Display on Microtiter Plates
AU - Bryan, Marian C.
AU - Plettenburg, Oliver
AU - Sears, Pamela
AU - Rabuka, David
AU - Wacowich-Sgarbi, Shirley
AU - Wong, Chi Huey
N1 - Funding Information: This research was supported by the National Institutes of Health and the Skaggs Funds.
PY - 2002/6
Y1 - 2002/6
N2 - New insight into the importance of carbohydrates in biological systems underscores the need for rapid synthetic and screening procedures for them. Development of an organic synthesis-compatible linker that would attach saccharides to microtiter plates was therefore undertaken to facilitate research in glycobiology. Galactosyllipids containing small, hydrophobic groups at the anomeric position were screened for noncovalent binding to microtiter plates. When the lipid component was a saturated hydrocarbon between 13 and 15 carbons in length, the monosaccharide showed complete retention after aqueous washing and could be utilized in biological assays. This alkyl chain was also successfully employed with more complex oligosaccharides in biological assays. In light of these findings, this method of attachment of oligosaccharides to microtiter plates should be highly efficacious to high-throughput synthesis and analyses of carbohydrates in biological assays.
AB - New insight into the importance of carbohydrates in biological systems underscores the need for rapid synthetic and screening procedures for them. Development of an organic synthesis-compatible linker that would attach saccharides to microtiter plates was therefore undertaken to facilitate research in glycobiology. Galactosyllipids containing small, hydrophobic groups at the anomeric position were screened for noncovalent binding to microtiter plates. When the lipid component was a saturated hydrocarbon between 13 and 15 carbons in length, the monosaccharide showed complete retention after aqueous washing and could be utilized in biological assays. This alkyl chain was also successfully employed with more complex oligosaccharides in biological assays. In light of these findings, this method of attachment of oligosaccharides to microtiter plates should be highly efficacious to high-throughput synthesis and analyses of carbohydrates in biological assays.
UR - http://www.scopus.com/inward/record.url?scp=0035990941&partnerID=8YFLogxK
U2 - 10.1016/S1074-5521(02)00155-2
DO - 10.1016/S1074-5521(02)00155-2
M3 - Article
C2 - 12079783
AN - SCOPUS:0035990941
VL - 9
SP - 713
EP - 720
JO - Chemistry and Biology
JF - Chemistry and Biology
SN - 1074-5521
IS - 6
ER -