Details
| Original language | English |
|---|---|
| Article number | 096007 |
| Journal | Journal of biomedical optics |
| Volume | 16 |
| Issue number | 9 |
| Publication status | Published - Sept 2011 |
Abstract
We introduce a new method of rotational image acquisition for four-dimensional (4D) optical coherence tomography (OCT) of beating embryonic chick hearts. The rotational axis and the central A-scan of the OCT are identical. An out-of-phase image sequence covering multiple heartbeats is acquired at every angle of an incremental rotation of the deflection mirrors of the OCT system. Image acquisition is accomplished after a rotation of 180°. Comparison of a displayed live M-mode of the central A-scan with a reference M-mode allows instant detection of translational movements of the embryo. For calculation of 4D data sets, we apply an image-based retrospective gating algorithm using the phase information of the common central A-scan present in all acquired images. This leads to cylindrical three-dimensional data sets for every time step of the cardiac cycle that can be used for 4D visualization. We demonstrate this approach and provide a video of a beating Hamburger and Hamilton stage 16 embryonic chick heart generated from a 4D OCT data set using rotational image acquisition.
Keywords
- cardiovascular development, in vivo imaging, optical coherence tomography
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Engineering(all)
- Biomedical Engineering
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In: Journal of biomedical optics, Vol. 16, No. 9, 096007, 09.2011.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Rotationally acquired four-dimensional optical coherence tomography of embryonic chick hearts using retrospective gating on the common central A-scan
AU - Happel, Christoph M.
AU - Thommes, Jan
AU - Thrane, Lars
AU - Männer, Jörg
AU - Ortmaier, Tobias
AU - Heimann, Bodo
AU - Yelbuz, Talat Mesud
N1 - Funding information: The authors acknowledge support by research grants from German Research Foundation (DFG; YE 42/3-1) and German Foundation for Cardiac Research (DSHF; F/19/07) to Professor Dr. Med. T. M. Yelbuz, and from the German Research Foundation (DFG; HE 2445/22-1) to Professor Dr.-Ing. B. Heimann, internal funding from the Department of Pediatric Cardiology and Intensive Care Medicine at Hannover Medical School, and internal funding from the Institute of Mechatronic Systems, imes at the Leibniz University of Hannover.
PY - 2011/9
Y1 - 2011/9
N2 - We introduce a new method of rotational image acquisition for four-dimensional (4D) optical coherence tomography (OCT) of beating embryonic chick hearts. The rotational axis and the central A-scan of the OCT are identical. An out-of-phase image sequence covering multiple heartbeats is acquired at every angle of an incremental rotation of the deflection mirrors of the OCT system. Image acquisition is accomplished after a rotation of 180°. Comparison of a displayed live M-mode of the central A-scan with a reference M-mode allows instant detection of translational movements of the embryo. For calculation of 4D data sets, we apply an image-based retrospective gating algorithm using the phase information of the common central A-scan present in all acquired images. This leads to cylindrical three-dimensional data sets for every time step of the cardiac cycle that can be used for 4D visualization. We demonstrate this approach and provide a video of a beating Hamburger and Hamilton stage 16 embryonic chick heart generated from a 4D OCT data set using rotational image acquisition.
AB - We introduce a new method of rotational image acquisition for four-dimensional (4D) optical coherence tomography (OCT) of beating embryonic chick hearts. The rotational axis and the central A-scan of the OCT are identical. An out-of-phase image sequence covering multiple heartbeats is acquired at every angle of an incremental rotation of the deflection mirrors of the OCT system. Image acquisition is accomplished after a rotation of 180°. Comparison of a displayed live M-mode of the central A-scan with a reference M-mode allows instant detection of translational movements of the embryo. For calculation of 4D data sets, we apply an image-based retrospective gating algorithm using the phase information of the common central A-scan present in all acquired images. This leads to cylindrical three-dimensional data sets for every time step of the cardiac cycle that can be used for 4D visualization. We demonstrate this approach and provide a video of a beating Hamburger and Hamilton stage 16 embryonic chick heart generated from a 4D OCT data set using rotational image acquisition.
KW - cardiovascular development
KW - in vivo imaging
KW - optical coherence tomography
UR - http://www.scopus.com/inward/record.url?scp=80052520891&partnerID=8YFLogxK
U2 - 10.1117/1.3622491
DO - 10.1117/1.3622491
M3 - Article
C2 - 21950921
AN - SCOPUS:80052520891
VL - 16
JO - Journal of biomedical optics
JF - Journal of biomedical optics
SN - 1083-3668
IS - 9
M1 - 096007
ER -