Details
| Original language | English |
|---|---|
| Article number | 315 |
| Journal | Frontiers in microbiology |
| Volume | 7 |
| Issue number | MAR |
| Publication status | Published - 16 Mar 2016 |
Abstract
The Hsp100/Clp protease complexes of Bacillus subtilis ClpXP and ClpCP are involved in the control of many interconnected developmental and stress response regulatory networks, including competence, redox stress response, and motility. Here we analyzed the role of regulatory proteolysis by ClpXP and ClpCP in motility development. We have demonstrated that ClpXP acts on the regulation of motility by controlling the levels of the oxidative and heat stress regulator Spx. We obtained evidence that upon oxidative stress Spx not only induces the thiol stress response, but also transiently represses the transcription of flagellar genes. Furthermore, we observed that in addition to the known impact of ClpCP via the ComK/FlgM-dependent pathway, ClpCP also affects flagellar gene expression via modulating the activity and levels of the global regulator DegU-P. This adds another layer to the intricate involvement of Clp mediated regulatory proteolysis in different gene expression programs, which may allow to integrate and coordinate different signals for a better-adjusted response to the changing environment of B. subtilis cells.
Keywords
- AAA+ proteins, Bacillus subtilis, ClpC, ClpP, ClpX, Motility, Regulatory proteolysis
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Medicine(all)
- Microbiology (medical)
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In: Frontiers in microbiology, Vol. 7, No. MAR, 315, 16.03.2016.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Role of Hsp100/Clp protease complexes in controlling the regulation of motility in Bacillus subtilis
AU - Molière, Noël
AU - Hoßmann, Jörn
AU - Schäfer, Heinrich
AU - Turgay, Kürsad
N1 - Publisher Copyright: © 2016 Molière, Hoßmann, Schäfer and Turgay. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2016/3/16
Y1 - 2016/3/16
N2 - The Hsp100/Clp protease complexes of Bacillus subtilis ClpXP and ClpCP are involved in the control of many interconnected developmental and stress response regulatory networks, including competence, redox stress response, and motility. Here we analyzed the role of regulatory proteolysis by ClpXP and ClpCP in motility development. We have demonstrated that ClpXP acts on the regulation of motility by controlling the levels of the oxidative and heat stress regulator Spx. We obtained evidence that upon oxidative stress Spx not only induces the thiol stress response, but also transiently represses the transcription of flagellar genes. Furthermore, we observed that in addition to the known impact of ClpCP via the ComK/FlgM-dependent pathway, ClpCP also affects flagellar gene expression via modulating the activity and levels of the global regulator DegU-P. This adds another layer to the intricate involvement of Clp mediated regulatory proteolysis in different gene expression programs, which may allow to integrate and coordinate different signals for a better-adjusted response to the changing environment of B. subtilis cells.
AB - The Hsp100/Clp protease complexes of Bacillus subtilis ClpXP and ClpCP are involved in the control of many interconnected developmental and stress response regulatory networks, including competence, redox stress response, and motility. Here we analyzed the role of regulatory proteolysis by ClpXP and ClpCP in motility development. We have demonstrated that ClpXP acts on the regulation of motility by controlling the levels of the oxidative and heat stress regulator Spx. We obtained evidence that upon oxidative stress Spx not only induces the thiol stress response, but also transiently represses the transcription of flagellar genes. Furthermore, we observed that in addition to the known impact of ClpCP via the ComK/FlgM-dependent pathway, ClpCP also affects flagellar gene expression via modulating the activity and levels of the global regulator DegU-P. This adds another layer to the intricate involvement of Clp mediated regulatory proteolysis in different gene expression programs, which may allow to integrate and coordinate different signals for a better-adjusted response to the changing environment of B. subtilis cells.
KW - AAA+ proteins
KW - Bacillus subtilis
KW - ClpC
KW - ClpP
KW - ClpX
KW - Motility
KW - Regulatory proteolysis
UR - http://www.scopus.com/inward/record.url?scp=84964370861&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2016.00315
DO - 10.3389/fmicb.2016.00315
M3 - Article
AN - SCOPUS:84964370861
VL - 7
JO - Frontiers in microbiology
JF - Frontiers in microbiology
SN - 1664-302X
IS - MAR
M1 - 315
ER -