Details
| Original language | English |
|---|---|
| Pages (from-to) | 1223-1230 |
| Number of pages | 8 |
| Journal | Pakistan Journal of Pharmaceutical Sciences |
| Volume | 29 |
| Issue number | 4 |
| Publication status | Published - Jul 2016 |
| Externally published | Yes |
Abstract
The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.
Keywords
- Aspergillus carbonarius, Chemical enhancers, Growth media, Pharmaceutical activities, Silient genes
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
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In: Pakistan Journal of Pharmaceutical Sciences, Vol. 29, No. 4, 07.2016, p. 1223-1230.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Role of growth media and chemical enhancers in secondary metabolites production from Aspergillus carbonarius (NRL-369) and their pharmaceutical potentials
AU - Khan, Abid Ali
AU - Bacha, Nafess
AU - Ahmad, Bashir
AU - Cox, R. J.
AU - Bakht, Jehan
PY - 2016/7
Y1 - 2016/7
N2 - The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.
AB - The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.
KW - Aspergillus carbonarius
KW - Chemical enhancers
KW - Growth media
KW - Pharmaceutical activities
KW - Silient genes
UR - http://www.scopus.com/inward/record.url?scp=84979659045&partnerID=8YFLogxK
M3 - Article
C2 - 27393435
AN - SCOPUS:84979659045
VL - 29
SP - 1223
EP - 1230
JO - Pakistan Journal of Pharmaceutical Sciences
JF - Pakistan Journal of Pharmaceutical Sciences
SN - 1011-601X
IS - 4
ER -