Details
| Original language | English |
|---|---|
| Article number | 144 |
| Journal | Horticulture Research |
| Volume | 7 |
| Issue number | 1 |
| Early online date | 1 Sept 2020 |
| Publication status | Published - 1 Dec 2020 |
Abstract
Growth depression of Rosa plants at sites previously used to cultivate the same or closely related species is a typical symptom of rose replant disease (RRD). Currently, limited information is available on the causes and the etiology of RRD compared to apple replant disease (ARD). Thus, this study aimed at analyzing growth characteristics, root morphology, and root metabolites, as well as microbial communities in the rhizosphere of the susceptible rootstock Rosacorymbifera ‘Laxa’ grown in RRD-affected soil from two sites (Heidgraben and Sangerhausen), either untreated or disinfected by γ-irradiation. In a greenhouse bioassay, plants developed significantly more biomass in the γ-irradiated than in the untreated soils of both sites. Several plant metabolites detected in R. corymbifera ‘Laxa’ roots were site- and treatment-dependent. Although aloesin was recorded in significantly higher concentrations in untreated than in γ-irradiated soils from Heidgraben, the concentrations of phenylalanine were significantly lower in roots from untreated soil of both sites. Rhizosphere microbial communities of 8-week-old plants were studied by sequencing of 16S rRNA, ITS, and cox gene fragments amplified from total community DNA. Supported by microscopic observations, sequences affiliated to the bacterial genus Streptomyces and the fungal genus Nectria were identified as potential causal agents of RRD in the soils investigated. The relative abundance of oomycetes belonging to the genus Pythiogeton showed a negative correlation to the growth of the plants. Overall, the RRD symptoms, the effects of soil treatments on the composition of the rhizosphere microbial community revealed striking similarities to findings related to ARD.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Horticulture Research, Vol. 7, No. 1, 144, 01.12.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Rhizosphere microbial communities associated to rose replant disease
T2 - links to plant growth and root metabolites
AU - Yim, B.
AU - Baumann, A.
AU - Grunewaldt-Stöcker, Gisela
AU - Liu, B.
AU - Beerhues, L.
AU - Zühlke, S.
AU - Sapp, M.
AU - Nesme, J.
AU - Sørensen, S. J.
AU - Smalla, Kornelia
AU - Winkelmann, Traud
N1 - Funding Information: We are deeply grateful for financial support by the Mathias-Tantau-Stiftung, Uetersen, Germany. B.L. and L.B. are also thankful to the Deutsche Forschungsgemeinschaft (DFG) for funding. We thank Florian Losch and Selahaddin Sezgin for instrumental analytical service, Dr. Andreas Wrede for providing us with both the stratified rose seeds originally obtained from Harald Klei nurseries and the soil from Heidgraben, and Thomas Hawel for providing us with the soil from Sangerhausen. We also thank our technicians for their help in the experimental setup and evaluations. Open access funding provided by Projekt DEAL.
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Growth depression of Rosa plants at sites previously used to cultivate the same or closely related species is a typical symptom of rose replant disease (RRD). Currently, limited information is available on the causes and the etiology of RRD compared to apple replant disease (ARD). Thus, this study aimed at analyzing growth characteristics, root morphology, and root metabolites, as well as microbial communities in the rhizosphere of the susceptible rootstock Rosacorymbifera ‘Laxa’ grown in RRD-affected soil from two sites (Heidgraben and Sangerhausen), either untreated or disinfected by γ-irradiation. In a greenhouse bioassay, plants developed significantly more biomass in the γ-irradiated than in the untreated soils of both sites. Several plant metabolites detected in R. corymbifera ‘Laxa’ roots were site- and treatment-dependent. Although aloesin was recorded in significantly higher concentrations in untreated than in γ-irradiated soils from Heidgraben, the concentrations of phenylalanine were significantly lower in roots from untreated soil of both sites. Rhizosphere microbial communities of 8-week-old plants were studied by sequencing of 16S rRNA, ITS, and cox gene fragments amplified from total community DNA. Supported by microscopic observations, sequences affiliated to the bacterial genus Streptomyces and the fungal genus Nectria were identified as potential causal agents of RRD in the soils investigated. The relative abundance of oomycetes belonging to the genus Pythiogeton showed a negative correlation to the growth of the plants. Overall, the RRD symptoms, the effects of soil treatments on the composition of the rhizosphere microbial community revealed striking similarities to findings related to ARD.
AB - Growth depression of Rosa plants at sites previously used to cultivate the same or closely related species is a typical symptom of rose replant disease (RRD). Currently, limited information is available on the causes and the etiology of RRD compared to apple replant disease (ARD). Thus, this study aimed at analyzing growth characteristics, root morphology, and root metabolites, as well as microbial communities in the rhizosphere of the susceptible rootstock Rosacorymbifera ‘Laxa’ grown in RRD-affected soil from two sites (Heidgraben and Sangerhausen), either untreated or disinfected by γ-irradiation. In a greenhouse bioassay, plants developed significantly more biomass in the γ-irradiated than in the untreated soils of both sites. Several plant metabolites detected in R. corymbifera ‘Laxa’ roots were site- and treatment-dependent. Although aloesin was recorded in significantly higher concentrations in untreated than in γ-irradiated soils from Heidgraben, the concentrations of phenylalanine were significantly lower in roots from untreated soil of both sites. Rhizosphere microbial communities of 8-week-old plants were studied by sequencing of 16S rRNA, ITS, and cox gene fragments amplified from total community DNA. Supported by microscopic observations, sequences affiliated to the bacterial genus Streptomyces and the fungal genus Nectria were identified as potential causal agents of RRD in the soils investigated. The relative abundance of oomycetes belonging to the genus Pythiogeton showed a negative correlation to the growth of the plants. Overall, the RRD symptoms, the effects of soil treatments on the composition of the rhizosphere microbial community revealed striking similarities to findings related to ARD.
UR - http://www.scopus.com/inward/record.url?scp=85090018074&partnerID=8YFLogxK
U2 - 10.1038/s41438-020-00365-2
DO - 10.1038/s41438-020-00365-2
M3 - Article
AN - SCOPUS:85090018074
VL - 7
JO - Horticulture Research
JF - Horticulture Research
SN - 2052-7276
IS - 1
M1 - 144
ER -