Details
| Original language | English |
|---|---|
| Pages (from-to) | 42-55 |
| Number of pages | 14 |
| Journal | Biomaterials |
| Volume | 86 |
| Early online date | 2 Feb 2016 |
| Publication status | Published - 1 Apr 2016 |
| Externally published | Yes |
Abstract
A library of hyperbranched polyglycerols (HPGs) functionalized with different mole fractions of zwitterionic sulfabetaine and cationic quaternary ammonium ligands was synthesized and characterized. A post-polymerization method was employed that utilized double bond moieties on the dendritic HPG for the coupling of thiol-terminated ligands via UV initiated thiol-ene "click" chemistry. The proportions of different ligands were precisely controlled by varying the ligand concentration during the irradiation process. The effect of the polymer library on hemostasis was investigated using whole human blood. It was found that polymer with ≥40% of alkenes converted to positive charges and the remainder to sulfabetaines caused hemagglutination at ≥1 mg/mL, without causing red blood cell lysis. The quaternary ammonium groups can interact with the negative charged sites on the membranes of erythrocytes, which provides the bioadhesion. The zwitterionic sulfabetaine evidently provides a hydration layer to partially mask the adverse effects that are likely to be caused by cationic moieties. The polymer was also found able to enhance platelet aggregation and activation in a concentration and positive charge density-dependent manner, which would contribute to initiating hemostasis. In a variety of other assays the material was found to be largely biocompatible. The polymer-induced hemostasis is obtained by a process independent of the normal blood clotting cascade but dependent on red blood cell agglutination, where the polymers promote hemostasis by linking erythrocytes together to form a lattice to entrap the cells.
Keywords
- Betaines, Bioadhesion, Hemostasis, Hyperbranched polyglycerol, Polycations
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Materials Science(all)
- Ceramics and Composites
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Materials Science(all)
- Biomaterials
- Engineering(all)
- Mechanics of Materials
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In: Biomaterials, Vol. 86, 01.04.2016, p. 42-55.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Reversible hemostatic properties of sulfabetaine/quaternary ammonium modified hyperbranched polyglycerol
AU - Wen, Jiying
AU - Weinhart, Marie
AU - Lai, Benjamin
AU - Kizhakkedathu, Jayachandran
AU - Brooks, Donald E.
N1 - Funding Information: This research is supported by Natural Sciences and Engineering Council of Canada (NSERC) ( RPGIN 238852-11 ) and the Canadian Blood Services ( 201209-293 ). JNK is a recipient of a Michael Smith Foundation for Health Research Career Scholar Award. MW is grateful to the German Research Foundation ( WE 5377/1-1 ) (DFG) for financial support. Publisher Copyright: © 2016 Elsevier Ltd.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - A library of hyperbranched polyglycerols (HPGs) functionalized with different mole fractions of zwitterionic sulfabetaine and cationic quaternary ammonium ligands was synthesized and characterized. A post-polymerization method was employed that utilized double bond moieties on the dendritic HPG for the coupling of thiol-terminated ligands via UV initiated thiol-ene "click" chemistry. The proportions of different ligands were precisely controlled by varying the ligand concentration during the irradiation process. The effect of the polymer library on hemostasis was investigated using whole human blood. It was found that polymer with ≥40% of alkenes converted to positive charges and the remainder to sulfabetaines caused hemagglutination at ≥1 mg/mL, without causing red blood cell lysis. The quaternary ammonium groups can interact with the negative charged sites on the membranes of erythrocytes, which provides the bioadhesion. The zwitterionic sulfabetaine evidently provides a hydration layer to partially mask the adverse effects that are likely to be caused by cationic moieties. The polymer was also found able to enhance platelet aggregation and activation in a concentration and positive charge density-dependent manner, which would contribute to initiating hemostasis. In a variety of other assays the material was found to be largely biocompatible. The polymer-induced hemostasis is obtained by a process independent of the normal blood clotting cascade but dependent on red blood cell agglutination, where the polymers promote hemostasis by linking erythrocytes together to form a lattice to entrap the cells.
AB - A library of hyperbranched polyglycerols (HPGs) functionalized with different mole fractions of zwitterionic sulfabetaine and cationic quaternary ammonium ligands was synthesized and characterized. A post-polymerization method was employed that utilized double bond moieties on the dendritic HPG for the coupling of thiol-terminated ligands via UV initiated thiol-ene "click" chemistry. The proportions of different ligands were precisely controlled by varying the ligand concentration during the irradiation process. The effect of the polymer library on hemostasis was investigated using whole human blood. It was found that polymer with ≥40% of alkenes converted to positive charges and the remainder to sulfabetaines caused hemagglutination at ≥1 mg/mL, without causing red blood cell lysis. The quaternary ammonium groups can interact with the negative charged sites on the membranes of erythrocytes, which provides the bioadhesion. The zwitterionic sulfabetaine evidently provides a hydration layer to partially mask the adverse effects that are likely to be caused by cationic moieties. The polymer was also found able to enhance platelet aggregation and activation in a concentration and positive charge density-dependent manner, which would contribute to initiating hemostasis. In a variety of other assays the material was found to be largely biocompatible. The polymer-induced hemostasis is obtained by a process independent of the normal blood clotting cascade but dependent on red blood cell agglutination, where the polymers promote hemostasis by linking erythrocytes together to form a lattice to entrap the cells.
KW - Betaines
KW - Bioadhesion
KW - Hemostasis
KW - Hyperbranched polyglycerol
KW - Polycations
UR - http://www.scopus.com/inward/record.url?scp=84975757453&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2016.01.067
DO - 10.1016/j.biomaterials.2016.01.067
M3 - Article
C2 - 26885776
AN - SCOPUS:84975757453
VL - 86
SP - 42
EP - 55
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
ER -