Details
| Original language | English |
|---|---|
| Pages (from-to) | 5318-28 |
| Number of pages | 11 |
| Journal | Journal of Biological Chemistry |
| Volume | 280 |
| Issue number | 7 |
| Publication status | Published - 18 Feb 2005 |
Abstract
Excitation imbalances between photosystem I and II generate redox signals in the thylakoid membrane of higher plants which induce acclimatory changes in the structure of the photosynthetic apparatus. They affect the accumulation of reaction center and light-harvesting proteins as well as chlorophylls a and b. In Arabidopsis thaliana the re-adjustment of photosystem stoichiometry is mainly mediated by changes in the number of photosystem I complexes, which are accompanied by corresponding changes in transcripts for plastid reaction center genes. Because chloroplast protein complexes contain also many nuclear encoded components we analyzed the impact of such photosynthetic redox signals on nuclear genes. Light shift experiments combined with application of the electron transport inhibitor 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea have been performed to induce defined redox signals in the thylakoid membrane. Using DNA macroarrays we assessed the impact of such redox signals on the expression of nuclear genes for chloroplast proteins. In addition, studies on mutants with lesions in cytosolic photoreceptors or in chloroplast-to-nucleus communication indicate that the defective components in the mutants are not essential for the perception and/or transduction of light-induced redox signals. A stable redox state of glutathione suggest that neither glutathione itself nor reactive oxygen species are involved in the observed regulation events pointing to the thylakoid membrane as the main origin of the regulatory pathways. Our data indicate a distinct role of photosynthetic redox signals in the cellular network regulating plant gene expression. These redox signals appear to act independently and/or above of cytosolic photoreceptor or known chloroplast-to-nucleus communication avenues.
Keywords
- Acclimatization/genetics, Arabidopsis/cytology, Arabidopsis Proteins/genetics, Base Sequence, Cell Nucleus/genetics, Chlorophyll/metabolism, Gene Expression Profiling, Gene Expression Regulation, Plant/radiation effects, Genes, Plant/genetics, Light, Light-Harvesting Protein Complexes/metabolism, Molecular Sequence Data, Mutation/genetics, Oligonucleotide Array Sequence Analysis, Oxidation-Reduction/radiation effects, Photosynthesis/radiation effects, Photosystem I Protein Complex/metabolism, Photosystem II Protein Complex/metabolism, Plant Proteins/metabolism, Plastids/genetics, Signal Transduction/genetics, Transcription, Genetic/genetics
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In: Journal of Biological Chemistry, Vol. 280, No. 7, 18.02.2005, p. 5318-28.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Retrograde plastid redox signals in the expression of nuclear genes for chloroplast proteins of Arabidopsis thaliana
AU - Fey, Vidal
AU - Wagner, Raik
AU - Braütigam, Katharina
AU - Wirtz, Markus
AU - Hell, Rüdiger
AU - Dietzmann, Angela
AU - Leister, Dario
AU - Oelmüller, Ralf
AU - Pfannschmidt, Thomas
PY - 2005/2/18
Y1 - 2005/2/18
N2 - Excitation imbalances between photosystem I and II generate redox signals in the thylakoid membrane of higher plants which induce acclimatory changes in the structure of the photosynthetic apparatus. They affect the accumulation of reaction center and light-harvesting proteins as well as chlorophylls a and b. In Arabidopsis thaliana the re-adjustment of photosystem stoichiometry is mainly mediated by changes in the number of photosystem I complexes, which are accompanied by corresponding changes in transcripts for plastid reaction center genes. Because chloroplast protein complexes contain also many nuclear encoded components we analyzed the impact of such photosynthetic redox signals on nuclear genes. Light shift experiments combined with application of the electron transport inhibitor 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea have been performed to induce defined redox signals in the thylakoid membrane. Using DNA macroarrays we assessed the impact of such redox signals on the expression of nuclear genes for chloroplast proteins. In addition, studies on mutants with lesions in cytosolic photoreceptors or in chloroplast-to-nucleus communication indicate that the defective components in the mutants are not essential for the perception and/or transduction of light-induced redox signals. A stable redox state of glutathione suggest that neither glutathione itself nor reactive oxygen species are involved in the observed regulation events pointing to the thylakoid membrane as the main origin of the regulatory pathways. Our data indicate a distinct role of photosynthetic redox signals in the cellular network regulating plant gene expression. These redox signals appear to act independently and/or above of cytosolic photoreceptor or known chloroplast-to-nucleus communication avenues.
AB - Excitation imbalances between photosystem I and II generate redox signals in the thylakoid membrane of higher plants which induce acclimatory changes in the structure of the photosynthetic apparatus. They affect the accumulation of reaction center and light-harvesting proteins as well as chlorophylls a and b. In Arabidopsis thaliana the re-adjustment of photosystem stoichiometry is mainly mediated by changes in the number of photosystem I complexes, which are accompanied by corresponding changes in transcripts for plastid reaction center genes. Because chloroplast protein complexes contain also many nuclear encoded components we analyzed the impact of such photosynthetic redox signals on nuclear genes. Light shift experiments combined with application of the electron transport inhibitor 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea have been performed to induce defined redox signals in the thylakoid membrane. Using DNA macroarrays we assessed the impact of such redox signals on the expression of nuclear genes for chloroplast proteins. In addition, studies on mutants with lesions in cytosolic photoreceptors or in chloroplast-to-nucleus communication indicate that the defective components in the mutants are not essential for the perception and/or transduction of light-induced redox signals. A stable redox state of glutathione suggest that neither glutathione itself nor reactive oxygen species are involved in the observed regulation events pointing to the thylakoid membrane as the main origin of the regulatory pathways. Our data indicate a distinct role of photosynthetic redox signals in the cellular network regulating plant gene expression. These redox signals appear to act independently and/or above of cytosolic photoreceptor or known chloroplast-to-nucleus communication avenues.
KW - Acclimatization/genetics
KW - Arabidopsis/cytology
KW - Arabidopsis Proteins/genetics
KW - Base Sequence
KW - Cell Nucleus/genetics
KW - Chlorophyll/metabolism
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Plant/radiation effects
KW - Genes, Plant/genetics
KW - Light
KW - Light-Harvesting Protein Complexes/metabolism
KW - Molecular Sequence Data
KW - Mutation/genetics
KW - Oligonucleotide Array Sequence Analysis
KW - Oxidation-Reduction/radiation effects
KW - Photosynthesis/radiation effects
KW - Photosystem I Protein Complex/metabolism
KW - Photosystem II Protein Complex/metabolism
KW - Plant Proteins/metabolism
KW - Plastids/genetics
KW - Signal Transduction/genetics
KW - Transcription, Genetic/genetics
U2 - 10.1074/jbc.M406358200
DO - 10.1074/jbc.M406358200
M3 - Article
C2 - 15561727
VL - 280
SP - 5318
EP - 5328
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 7
ER -