Respiratory chain supercomplexes in plant mitochondria

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Original languageEnglish
Pages (from-to)937-942
Number of pages6
JournalPlant physiology and biochemistry
Volume42
Issue number12
Publication statusPublished - 21 Dec 2004

Abstract

Supercomplexes are defined associations of protein complexes, which are important for several cellular functions. This "quintenary" organization level of protein structure recently was also described for the respiratory chain of plant mitochondria. Except succinate dehydrogenase (complex II), all complexes of the oxidative phosphorylation (OXPOS) system (complexes I, III, IV and V) were found to form part of supercomplexes. Compositions of these supramolecular structures were systematically investigated using digitonin solubilizations of mitochondrial fractions and two-dimensional Blue-native (BN) polyacrylamide gel electrophoresis. The most abundant supercomplex of plant mitochondria includes complexes I and III at a 1:2 ratio (I1 + III2 supercomplex). Furthermore, some supercomplexes of lower abundance could be described, which have I2 + III4, V 2, III2 + IV1-2, and I1 + III 2 + IV1-4 compositions. Supercomplexes consisting of complexes I plus III plus IV were proposed to be called "respirasome", because they autonomously can carry out respiration in the presence of ubiquinone and cytochrome c. Plant specific alternative oxidoreductases of the respiratory chain were not associated with supercomplexes under all experimental conditions tested. However, formation of supercomplexes possibly indirectly regulates alternative respiratory pathways in plant mitochondria on the basis of electron channeling. In this review, procedures to characterize the supermolecular organization of the plant respiratory chain and results concerning supercomplex structure and function are summarized and discussed.

Keywords

    Arabidopsis, Blue-native polyacrylamide gel electrophoresis, Mitochondria, Respiratory chain, Supercomplexes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Agricultural and Biological Sciences(all)
  • Plant Science

Cite this

Respiratory chain supercomplexes in plant mitochondria. / Eubel, Holger; Heinemeyer, Jesco; Sunderhaus, Stephanie et al.
In: Plant physiology and biochemistry, Vol. 42, No. 12, 21.12.2004, p. 937-942.

Research output: Contribution to journalSurvey paperResearchpeer review

Eubel H, Heinemeyer J, Sunderhaus S, Braun HP. Respiratory chain supercomplexes in plant mitochondria. Plant physiology and biochemistry. 2004 Dec 21;42(12):937-942. doi: 10.1016/j.plaphy.2004.09.010
Eubel, Holger ; Heinemeyer, Jesco ; Sunderhaus, Stephanie et al. / Respiratory chain supercomplexes in plant mitochondria. In: Plant physiology and biochemistry. 2004 ; Vol. 42, No. 12. pp. 937-942.
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T1 - Respiratory chain supercomplexes in plant mitochondria

AU - Eubel, Holger

AU - Heinemeyer, Jesco

AU - Sunderhaus, Stephanie

AU - Braun, Hans Peter

N1 - Funding information: Our research is supported by the Deutsche Forschungsgemeinschaft (grant BR 1829-7/1).

PY - 2004/12/21

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N2 - Supercomplexes are defined associations of protein complexes, which are important for several cellular functions. This "quintenary" organization level of protein structure recently was also described for the respiratory chain of plant mitochondria. Except succinate dehydrogenase (complex II), all complexes of the oxidative phosphorylation (OXPOS) system (complexes I, III, IV and V) were found to form part of supercomplexes. Compositions of these supramolecular structures were systematically investigated using digitonin solubilizations of mitochondrial fractions and two-dimensional Blue-native (BN) polyacrylamide gel electrophoresis. The most abundant supercomplex of plant mitochondria includes complexes I and III at a 1:2 ratio (I1 + III2 supercomplex). Furthermore, some supercomplexes of lower abundance could be described, which have I2 + III4, V 2, III2 + IV1-2, and I1 + III 2 + IV1-4 compositions. Supercomplexes consisting of complexes I plus III plus IV were proposed to be called "respirasome", because they autonomously can carry out respiration in the presence of ubiquinone and cytochrome c. Plant specific alternative oxidoreductases of the respiratory chain were not associated with supercomplexes under all experimental conditions tested. However, formation of supercomplexes possibly indirectly regulates alternative respiratory pathways in plant mitochondria on the basis of electron channeling. In this review, procedures to characterize the supermolecular organization of the plant respiratory chain and results concerning supercomplex structure and function are summarized and discussed.

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KW - Arabidopsis

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