Details
| Original language | English |
|---|---|
| Pages (from-to) | 937-942 |
| Number of pages | 6 |
| Journal | Plant physiology and biochemistry |
| Volume | 42 |
| Issue number | 12 |
| Publication status | Published - 21 Dec 2004 |
Abstract
Supercomplexes are defined associations of protein complexes, which are important for several cellular functions. This "quintenary" organization level of protein structure recently was also described for the respiratory chain of plant mitochondria. Except succinate dehydrogenase (complex II), all complexes of the oxidative phosphorylation (OXPOS) system (complexes I, III, IV and V) were found to form part of supercomplexes. Compositions of these supramolecular structures were systematically investigated using digitonin solubilizations of mitochondrial fractions and two-dimensional Blue-native (BN) polyacrylamide gel electrophoresis. The most abundant supercomplex of plant mitochondria includes complexes I and III at a 1:2 ratio (I1 + III2 supercomplex). Furthermore, some supercomplexes of lower abundance could be described, which have I2 + III4, V 2, III2 + IV1-2, and I1 + III 2 + IV1-4 compositions. Supercomplexes consisting of complexes I plus III plus IV were proposed to be called "respirasome", because they autonomously can carry out respiration in the presence of ubiquinone and cytochrome c. Plant specific alternative oxidoreductases of the respiratory chain were not associated with supercomplexes under all experimental conditions tested. However, formation of supercomplexes possibly indirectly regulates alternative respiratory pathways in plant mitochondria on the basis of electron channeling. In this review, procedures to characterize the supermolecular organization of the plant respiratory chain and results concerning supercomplex structure and function are summarized and discussed.
Keywords
- Arabidopsis, Blue-native polyacrylamide gel electrophoresis, Mitochondria, Respiratory chain, Supercomplexes
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant physiology and biochemistry, Vol. 42, No. 12, 21.12.2004, p. 937-942.
Research output: Contribution to journal › Survey paper › Research › peer review
}
TY - JOUR
T1 - Respiratory chain supercomplexes in plant mitochondria
AU - Eubel, Holger
AU - Heinemeyer, Jesco
AU - Sunderhaus, Stephanie
AU - Braun, Hans Peter
N1 - Funding information: Our research is supported by the Deutsche Forschungsgemeinschaft (grant BR 1829-7/1).
PY - 2004/12/21
Y1 - 2004/12/21
N2 - Supercomplexes are defined associations of protein complexes, which are important for several cellular functions. This "quintenary" organization level of protein structure recently was also described for the respiratory chain of plant mitochondria. Except succinate dehydrogenase (complex II), all complexes of the oxidative phosphorylation (OXPOS) system (complexes I, III, IV and V) were found to form part of supercomplexes. Compositions of these supramolecular structures were systematically investigated using digitonin solubilizations of mitochondrial fractions and two-dimensional Blue-native (BN) polyacrylamide gel electrophoresis. The most abundant supercomplex of plant mitochondria includes complexes I and III at a 1:2 ratio (I1 + III2 supercomplex). Furthermore, some supercomplexes of lower abundance could be described, which have I2 + III4, V 2, III2 + IV1-2, and I1 + III 2 + IV1-4 compositions. Supercomplexes consisting of complexes I plus III plus IV were proposed to be called "respirasome", because they autonomously can carry out respiration in the presence of ubiquinone and cytochrome c. Plant specific alternative oxidoreductases of the respiratory chain were not associated with supercomplexes under all experimental conditions tested. However, formation of supercomplexes possibly indirectly regulates alternative respiratory pathways in plant mitochondria on the basis of electron channeling. In this review, procedures to characterize the supermolecular organization of the plant respiratory chain and results concerning supercomplex structure and function are summarized and discussed.
AB - Supercomplexes are defined associations of protein complexes, which are important for several cellular functions. This "quintenary" organization level of protein structure recently was also described for the respiratory chain of plant mitochondria. Except succinate dehydrogenase (complex II), all complexes of the oxidative phosphorylation (OXPOS) system (complexes I, III, IV and V) were found to form part of supercomplexes. Compositions of these supramolecular structures were systematically investigated using digitonin solubilizations of mitochondrial fractions and two-dimensional Blue-native (BN) polyacrylamide gel electrophoresis. The most abundant supercomplex of plant mitochondria includes complexes I and III at a 1:2 ratio (I1 + III2 supercomplex). Furthermore, some supercomplexes of lower abundance could be described, which have I2 + III4, V 2, III2 + IV1-2, and I1 + III 2 + IV1-4 compositions. Supercomplexes consisting of complexes I plus III plus IV were proposed to be called "respirasome", because they autonomously can carry out respiration in the presence of ubiquinone and cytochrome c. Plant specific alternative oxidoreductases of the respiratory chain were not associated with supercomplexes under all experimental conditions tested. However, formation of supercomplexes possibly indirectly regulates alternative respiratory pathways in plant mitochondria on the basis of electron channeling. In this review, procedures to characterize the supermolecular organization of the plant respiratory chain and results concerning supercomplex structure and function are summarized and discussed.
KW - Arabidopsis
KW - Blue-native polyacrylamide gel electrophoresis
KW - Mitochondria
KW - Respiratory chain
KW - Supercomplexes
UR - http://www.scopus.com/inward/record.url?scp=13444292066&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2004.09.010
DO - 10.1016/j.plaphy.2004.09.010
M3 - Survey paper
C2 - 15707832
AN - SCOPUS:13444292066
VL - 42
SP - 937
EP - 942
JO - Plant physiology and biochemistry
JF - Plant physiology and biochemistry
SN - 0981-9428
IS - 12
ER -