Details
| Original language | English |
|---|---|
| Pages (from-to) | 442-450 |
| Number of pages | 9 |
| Journal | Scientia horticulturae |
| Volume | 125 |
| Issue number | 3 |
| Early online date | 7 May 2010 |
| Publication status | Published - 28 Jun 2010 |
Abstract
The present study is the first report of the establishment of embryogenic callus cultures from seedling tissue, the regeneration of plants via somatic embryogenesis and the development of a regeneration system from protoplast to plant, using three wild species of Cyclamen, Cyclamen graecum Link, Cyclamen mirabile Hildebrand, Cyclamen trochopteranthum Schwarz (syn. Cyclamen alpinum hort. Dammann ex Sprenger). The ability to form embryogenic callus and to regenerate via somatic embryogenesis was strongly genotype-dependent for each species. From 0.5 g callus, up to 1461 somatic embryos were formed in the case of C. mirabile. Culture media with different concentrations of plant growth regulators, CaCl2 and activated charcoal significantly influenced embryo formation in this species. Up to 1.4 × 106 protoplasts were isolated from 1 g of C. graecum cell suspension. Diverse growth responses of the protoplasts in two embedding agents, agarose and alginate, were observed for the different Cyclamen species. These specific growth characteristics could be used as a selection marker for future fusion experiments. From both protoplast culture systems, somatic embryos were regenerated, grown to plantlets and acclimatised to greenhouse conditions.
Keywords
- Agarose, Alginate, Cyclamen alpinum, Cyclamen graecum, Cyclamen mirabile, Ornamental plant, Protoplast isolation, Vegetative propagation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Scientia horticulturae, Vol. 125, No. 3, 28.06.2010, p. 442-450.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Regeneration of different Cyclamen species via somatic embryogenesis from callus, suspension cultures and protoplasts
AU - Prange, Anika Nadja Sabine
AU - Bartsch, Melanie
AU - Serek, Margrethe
AU - Winkelmann, Traud
PY - 2010/6/28
Y1 - 2010/6/28
N2 - The present study is the first report of the establishment of embryogenic callus cultures from seedling tissue, the regeneration of plants via somatic embryogenesis and the development of a regeneration system from protoplast to plant, using three wild species of Cyclamen, Cyclamen graecum Link, Cyclamen mirabile Hildebrand, Cyclamen trochopteranthum Schwarz (syn. Cyclamen alpinum hort. Dammann ex Sprenger). The ability to form embryogenic callus and to regenerate via somatic embryogenesis was strongly genotype-dependent for each species. From 0.5 g callus, up to 1461 somatic embryos were formed in the case of C. mirabile. Culture media with different concentrations of plant growth regulators, CaCl2 and activated charcoal significantly influenced embryo formation in this species. Up to 1.4 × 106 protoplasts were isolated from 1 g of C. graecum cell suspension. Diverse growth responses of the protoplasts in two embedding agents, agarose and alginate, were observed for the different Cyclamen species. These specific growth characteristics could be used as a selection marker for future fusion experiments. From both protoplast culture systems, somatic embryos were regenerated, grown to plantlets and acclimatised to greenhouse conditions.
AB - The present study is the first report of the establishment of embryogenic callus cultures from seedling tissue, the regeneration of plants via somatic embryogenesis and the development of a regeneration system from protoplast to plant, using three wild species of Cyclamen, Cyclamen graecum Link, Cyclamen mirabile Hildebrand, Cyclamen trochopteranthum Schwarz (syn. Cyclamen alpinum hort. Dammann ex Sprenger). The ability to form embryogenic callus and to regenerate via somatic embryogenesis was strongly genotype-dependent for each species. From 0.5 g callus, up to 1461 somatic embryos were formed in the case of C. mirabile. Culture media with different concentrations of plant growth regulators, CaCl2 and activated charcoal significantly influenced embryo formation in this species. Up to 1.4 × 106 protoplasts were isolated from 1 g of C. graecum cell suspension. Diverse growth responses of the protoplasts in two embedding agents, agarose and alginate, were observed for the different Cyclamen species. These specific growth characteristics could be used as a selection marker for future fusion experiments. From both protoplast culture systems, somatic embryos were regenerated, grown to plantlets and acclimatised to greenhouse conditions.
KW - Agarose
KW - Alginate
KW - Cyclamen alpinum
KW - Cyclamen graecum
KW - Cyclamen mirabile
KW - Ornamental plant
KW - Protoplast isolation
KW - Vegetative propagation
UR - http://www.scopus.com/inward/record.url?scp=77953129287&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2010.04.018
DO - 10.1016/j.scienta.2010.04.018
M3 - Article
AN - SCOPUS:77953129287
VL - 125
SP - 442
EP - 450
JO - Scientia horticulturae
JF - Scientia horticulturae
SN - 0304-4238
IS - 3
ER -