Details
| Original language | English |
|---|---|
| Title of host publication | Advanced Microscopy Techniques |
| Publication status | Published - 1 Jul 2009 |
| Externally published | Yes |
| Event | Advanced Microscopy Techniques - Munich, Germany Duration: 14 Jun 2009 → 16 Jun 2009 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 7367 |
| ISSN (Print) | 1605-7422 |
Abstract
Cross-linking of the cornea with application of Ribovlavin and UV-A light is an evolving clinical treatment of the eye disease keratoconus. Despite the positive clinical track record of corneal cross-linking, the complex wound healing process after the treatment is still under investigation. In this study an animal model was used to clarify the state of wound healing 5 weeks after treatment. Cross-linked rabbit corneae were imaged with reflective confocal laser scanning and nonlinear microscopy, namely second harmonic imaging microscopy (SHIM) and two-photon excited autofluorescence. First results show that the NAD(P) H-autofluorescence of the corneal keratocytes and their scattering signal still show a signature of the treatment five weeks after the cross-linking procedure. The SHIM signals show the structural morphology of the fibrous collagen sheets in the stroma of the cornea. SHIM detected in the forward direction differs substantially from backward SHIM, but no signature of treatment was found in both detection channels of the SHIM signal.
Keywords
- Collagen, Cornea, Cross-linking, Nonlinear microscopy, Reflective confocal laser scanning microscopy, Second harmonic generation, Two-photon microscopy
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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- BibTeX
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Advanced Microscopy Techniques. 2009. 736719 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7367).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Reflective confocal laser scanning microscopy and nonlinear microscopy of cross-linked rabbit cornea
AU - Krüger, Alexander
AU - Hovakimyan, Marina
AU - Ramirez, Diego F.
AU - Stachs, Oliver
AU - Guthoff, Rudolf F.
AU - Heisterkamp, Alexander
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Cross-linking of the cornea with application of Ribovlavin and UV-A light is an evolving clinical treatment of the eye disease keratoconus. Despite the positive clinical track record of corneal cross-linking, the complex wound healing process after the treatment is still under investigation. In this study an animal model was used to clarify the state of wound healing 5 weeks after treatment. Cross-linked rabbit corneae were imaged with reflective confocal laser scanning and nonlinear microscopy, namely second harmonic imaging microscopy (SHIM) and two-photon excited autofluorescence. First results show that the NAD(P) H-autofluorescence of the corneal keratocytes and their scattering signal still show a signature of the treatment five weeks after the cross-linking procedure. The SHIM signals show the structural morphology of the fibrous collagen sheets in the stroma of the cornea. SHIM detected in the forward direction differs substantially from backward SHIM, but no signature of treatment was found in both detection channels of the SHIM signal.
AB - Cross-linking of the cornea with application of Ribovlavin and UV-A light is an evolving clinical treatment of the eye disease keratoconus. Despite the positive clinical track record of corneal cross-linking, the complex wound healing process after the treatment is still under investigation. In this study an animal model was used to clarify the state of wound healing 5 weeks after treatment. Cross-linked rabbit corneae were imaged with reflective confocal laser scanning and nonlinear microscopy, namely second harmonic imaging microscopy (SHIM) and two-photon excited autofluorescence. First results show that the NAD(P) H-autofluorescence of the corneal keratocytes and their scattering signal still show a signature of the treatment five weeks after the cross-linking procedure. The SHIM signals show the structural morphology of the fibrous collagen sheets in the stroma of the cornea. SHIM detected in the forward direction differs substantially from backward SHIM, but no signature of treatment was found in both detection channels of the SHIM signal.
KW - Collagen
KW - Cornea
KW - Cross-linking
KW - Nonlinear microscopy
KW - Reflective confocal laser scanning microscopy
KW - Second harmonic generation
KW - Two-photon microscopy
UR - http://www.scopus.com/inward/record.url?scp=70349990489&partnerID=8YFLogxK
U2 - 10.1117/12.831478
DO - 10.1117/12.831478
M3 - Conference contribution
AN - SCOPUS:70349990489
SN - 9780819476432
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Advanced Microscopy Techniques
T2 - Advanced Microscopy Techniques
Y2 - 14 June 2009 through 16 June 2009
ER -