Details
| Original language | English |
|---|---|
| Pages (from-to) | 1503-1508 |
| Number of pages | 6 |
| Journal | Fresenius environmental bulletin |
| Volume | 16 |
| Issue number | 11 B |
| Publication status | Published - 2007 |
Abstract
Foam fractionation was used to concentrate and to purify extracellular alkaline lipases produced by Fusarium spec. in submerged cultures supplemented with Tween 80. Gas flow rate and pH-value proved to be crucial process parameters. Maximum recovery rates of lipolytic activity were obtained at 60 mL min-1 N2 and pH 8.5. Under these conditions, > 90% of the total lipase activity was recovered in the foam with enrichment and purification factors of 8.8 and 5.9, respectively. The secreted lipases were characterized biochemically by means of IEF electrophoresis with activity staining and electrospray tandem mass spectrometry.
Keywords
- Activity staining, Alkaline lipase, Foam fractionation, Fusarium spec.
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
- Environmental Science(all)
- Waste Management and Disposal
- Environmental Science(all)
- Pollution
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In: Fresenius environmental bulletin, Vol. 16, No. 11 B, 2007, p. 1503-1508.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Recovery of extracellular alkaline lipases of Fusarium spec. by foam fractionation
AU - Dos Prazeres, Janaina N.
AU - Simiqueli, Ana P.
AU - Pastore, Glaucia M.
AU - Linke, Diana
AU - Zorn, Holger
AU - Nimtz, Manfred
AU - Berger, Ralf G.
PY - 2007
Y1 - 2007
N2 - Foam fractionation was used to concentrate and to purify extracellular alkaline lipases produced by Fusarium spec. in submerged cultures supplemented with Tween 80. Gas flow rate and pH-value proved to be crucial process parameters. Maximum recovery rates of lipolytic activity were obtained at 60 mL min-1 N2 and pH 8.5. Under these conditions, > 90% of the total lipase activity was recovered in the foam with enrichment and purification factors of 8.8 and 5.9, respectively. The secreted lipases were characterized biochemically by means of IEF electrophoresis with activity staining and electrospray tandem mass spectrometry.
AB - Foam fractionation was used to concentrate and to purify extracellular alkaline lipases produced by Fusarium spec. in submerged cultures supplemented with Tween 80. Gas flow rate and pH-value proved to be crucial process parameters. Maximum recovery rates of lipolytic activity were obtained at 60 mL min-1 N2 and pH 8.5. Under these conditions, > 90% of the total lipase activity was recovered in the foam with enrichment and purification factors of 8.8 and 5.9, respectively. The secreted lipases were characterized biochemically by means of IEF electrophoresis with activity staining and electrospray tandem mass spectrometry.
KW - Activity staining
KW - Alkaline lipase
KW - Foam fractionation
KW - Fusarium spec.
UR - http://www.scopus.com/inward/record.url?scp=37849047973&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:37849047973
VL - 16
SP - 1503
EP - 1508
JO - Fresenius environmental bulletin
JF - Fresenius environmental bulletin
SN - 1018-4619
IS - 11 B
ER -