Details
| Original language | English |
|---|---|
| Pages (from-to) | 6402-6411 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 1218 |
| Issue number | 37 |
| Early online date | 18 Jul 2011 |
| Publication status | Published - 16 Sept 2011 |
Abstract
In the first part of this work adsorption isotherm parameters were acquired to describe the migration of recombinant streptokinase in Butyl Sepharose columns at different salt concentrations. Based on these results, a simulated moving bed (SMB) chromatographic process was designed and realised, which exploits a two-step salt gradient and allows the continuous separation of streptokinase from contaminants present in a clarified Escherichia coli cell lysate solution. This second part describes the design of the three-zone open-loop gradient SMB process applying both equilibrium theory and an equilibrium stage model and presents results of a series of experiments aiming to obtain pure streptokinase. Moreover, the potential of the SMB process and the design approach are evaluated.
Keywords
- Hydrophobic interaction chromatography, Streptokinase, Two-step gradient open-loop simulated moving bed chromatography
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Organic Chemistry
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In: Journal of Chromatography A, Vol. 1218, No. 37, 16.09.2011, p. 6402-6411.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Recombinant protein purification using gradient assisted simulated moving bed hydrophobic interaction chromatography. Part II
T2 - Process design and experimental validation
AU - Gueorguieva, Ludmila
AU - Palani, Sivakumar
AU - Rinas, Ursula
AU - Jayaraman, Guhan
AU - Seidel-Morgenstern, Andreas
N1 - Funding Information: We acknowledge the support of the Bioprocess Engineering Group at the MPI and the Leibniz Institute for Neurobiology (both in Magdeburg). S.P. acknowledges the financial support of Deutscher Akademischer Austauschdienst (DAAD) and the travel grant to the “HPLC 2009” from GDCh . The support of Deutsche Forschungsgemeinschaft via “ SFB 578 ” is also gratefully acknowledged.
PY - 2011/9/16
Y1 - 2011/9/16
N2 - In the first part of this work adsorption isotherm parameters were acquired to describe the migration of recombinant streptokinase in Butyl Sepharose columns at different salt concentrations. Based on these results, a simulated moving bed (SMB) chromatographic process was designed and realised, which exploits a two-step salt gradient and allows the continuous separation of streptokinase from contaminants present in a clarified Escherichia coli cell lysate solution. This second part describes the design of the three-zone open-loop gradient SMB process applying both equilibrium theory and an equilibrium stage model and presents results of a series of experiments aiming to obtain pure streptokinase. Moreover, the potential of the SMB process and the design approach are evaluated.
AB - In the first part of this work adsorption isotherm parameters were acquired to describe the migration of recombinant streptokinase in Butyl Sepharose columns at different salt concentrations. Based on these results, a simulated moving bed (SMB) chromatographic process was designed and realised, which exploits a two-step salt gradient and allows the continuous separation of streptokinase from contaminants present in a clarified Escherichia coli cell lysate solution. This second part describes the design of the three-zone open-loop gradient SMB process applying both equilibrium theory and an equilibrium stage model and presents results of a series of experiments aiming to obtain pure streptokinase. Moreover, the potential of the SMB process and the design approach are evaluated.
KW - Hydrophobic interaction chromatography
KW - Streptokinase
KW - Two-step gradient open-loop simulated moving bed chromatography
UR - http://www.scopus.com/inward/record.url?scp=80051870442&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2011.07.008
DO - 10.1016/j.chroma.2011.07.008
M3 - Article
C2 - 21824621
AN - SCOPUS:80051870442
VL - 1218
SP - 6402
EP - 6411
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 37
ER -