Details
| Original language | English |
|---|---|
| Pages (from-to) | 1517-1529 |
| Number of pages | 13 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 98 |
| Issue number | 4 |
| Early online date | 21 Dec 2013 |
| Publication status | Published - Feb 2014 |
| Externally published | Yes |
Abstract
Cofactor-dependent enzymes catalyze a broad range of synthetically useful transformations. However, the cofactor requirement also poses economic and practical challenges for the application of these biocatalysts. For three decades, considerable research effort has been devoted to the development of reliable in situ regeneration methods for the most commonly employed cofactors, particularly NADH and NADPH. Today, researchers can choose from a plethora of options, and oxidoreductases are routinely employed even on industrial scale. Nevertheless, more efficient cofactor regeneration methods are still being developed, with the aim of achieving better atom economy, simpler reaction setups, and higher productivities. Besides, cofactor dependence has been recognized as an opportunity to confer novel reactivity upon enzymes by engineering their cofactors, and to couple (redox) biotransformations in multi-enzyme cascade systems. These novel concepts will help to further establish cofactor-dependent biotransformations as an attractive option for the synthesis of biologically active compounds, chiral building blocks, and bio-based platform molecules.
Keywords
- Biocatalysis, Cascade reaction, Cofactor regeneration, Designer cells, Enzyme cofactor, Non-natural cofactors
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 98, No. 4, 02.2014, p. 1517-1529.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Recent trends and novel concepts in cofactor-dependent biotransformations
AU - Kara, Selin
AU - Schrittwieser, Joerg H.
AU - Hollmann, Frank
AU - Ansorge-Schumacher, Marion B.
N1 - Funding Information: Acknowledgments JHS thanks the Austrian Science Fund (FWF) for financial support in the form of an “Erwin Schrödinger” fellowship (J3244-N17).
PY - 2014/2
Y1 - 2014/2
N2 - Cofactor-dependent enzymes catalyze a broad range of synthetically useful transformations. However, the cofactor requirement also poses economic and practical challenges for the application of these biocatalysts. For three decades, considerable research effort has been devoted to the development of reliable in situ regeneration methods for the most commonly employed cofactors, particularly NADH and NADPH. Today, researchers can choose from a plethora of options, and oxidoreductases are routinely employed even on industrial scale. Nevertheless, more efficient cofactor regeneration methods are still being developed, with the aim of achieving better atom economy, simpler reaction setups, and higher productivities. Besides, cofactor dependence has been recognized as an opportunity to confer novel reactivity upon enzymes by engineering their cofactors, and to couple (redox) biotransformations in multi-enzyme cascade systems. These novel concepts will help to further establish cofactor-dependent biotransformations as an attractive option for the synthesis of biologically active compounds, chiral building blocks, and bio-based platform molecules.
AB - Cofactor-dependent enzymes catalyze a broad range of synthetically useful transformations. However, the cofactor requirement also poses economic and practical challenges for the application of these biocatalysts. For three decades, considerable research effort has been devoted to the development of reliable in situ regeneration methods for the most commonly employed cofactors, particularly NADH and NADPH. Today, researchers can choose from a plethora of options, and oxidoreductases are routinely employed even on industrial scale. Nevertheless, more efficient cofactor regeneration methods are still being developed, with the aim of achieving better atom economy, simpler reaction setups, and higher productivities. Besides, cofactor dependence has been recognized as an opportunity to confer novel reactivity upon enzymes by engineering their cofactors, and to couple (redox) biotransformations in multi-enzyme cascade systems. These novel concepts will help to further establish cofactor-dependent biotransformations as an attractive option for the synthesis of biologically active compounds, chiral building blocks, and bio-based platform molecules.
KW - Biocatalysis
KW - Cascade reaction
KW - Cofactor regeneration
KW - Designer cells
KW - Enzyme cofactor
KW - Non-natural cofactors
UR - http://www.scopus.com/inward/record.url?scp=84894372245&partnerID=8YFLogxK
U2 - 10.1007/s00253-013-5441-5
DO - 10.1007/s00253-013-5441-5
M3 - Review article
C2 - 24362856
AN - SCOPUS:84894372245
VL - 98
SP - 1517
EP - 1529
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 4
ER -