Details
| Original language | English |
|---|---|
| Pages (from-to) | 739-783 |
| Number of pages | 45 |
| Journal | Biotechnology advances |
| Volume | 36 |
| Issue number | 3 |
| Early online date | 5 Feb 2018 |
| Publication status | Published - May 2018 |
Abstract
High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
Keywords
- Aspergillus spp., Combinatorial biosynthesis, DNA assembly, Heterologous expression, Microbial secondary metabolites, Pathway engineering, Promoter Regions, Genetic, Multigene Family, Bridged Bicyclo Compounds, Heterocyclic, Cloning, Molecular/methods, Computational Biology/methods, Microorganisms, Genetically-Modified, Tricarboxylic Acids, Lovastatin/genetics, Polyketides/metabolism, Polymerase Chain Reaction/methods, Recombination, Genetic, Genetic Engineering/methods, Secondary Metabolism/genetics, Biosynthetic Pathways/genetics, Aspergillus/genetics
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
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In: Biotechnology advances, Vol. 36, No. 3, 05.2018, p. 739-783.
Research output: Contribution to journal › Article › Research
}
TY - JOUR
T1 - Recent advances in reconstructing microbial secondary metabolites biosynthesis in Aspergillus spp.
AU - He, Yi
AU - Wang, Bin
AU - Chen, Wanping
AU - Cox, Russell J.
AU - He, Jingren
AU - Chen, Fusheng
N1 - © 2018 Elsevier Inc. All rights reserved.
PY - 2018/5
Y1 - 2018/5
N2 - High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
AB - High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also discuss the technological challenges and successes in regard to heterologous host selection and DNA assembly behind the reconstruction of microbial secondary metabolite biosynthesis.
KW - Aspergillus spp.
KW - Combinatorial biosynthesis
KW - DNA assembly
KW - Heterologous expression
KW - Microbial secondary metabolites
KW - Pathway engineering
KW - Promoter Regions, Genetic
KW - Multigene Family
KW - Bridged Bicyclo Compounds, Heterocyclic
KW - Cloning, Molecular/methods
KW - Computational Biology/methods
KW - Microorganisms, Genetically-Modified
KW - Tricarboxylic Acids
KW - Lovastatin/genetics
KW - Polyketides/metabolism
KW - Polymerase Chain Reaction/methods
KW - Recombination, Genetic
KW - Genetic Engineering/methods
KW - Secondary Metabolism/genetics
KW - Biosynthetic Pathways/genetics
KW - Aspergillus/genetics
UR - http://www.scopus.com/inward/record.url?scp=85042365902&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2018.02.001
DO - 10.1016/j.biotechadv.2018.02.001
M3 - Article
C2 - 29421302
AN - SCOPUS:85042365902
VL - 36
SP - 739
EP - 783
JO - Biotechnology advances
JF - Biotechnology advances
SN - 0734-9750
IS - 3
ER -