Details
| Original language | English |
|---|---|
| Pages (from-to) | 171-177 |
| Number of pages | 7 |
| Journal | Journal of biotechnology |
| Volume | 257 |
| Publication status | Published - 25 Jan 2017 |
Abstract
Protein A, which is secreted by and displayed on the cell membrane of Staphylococcus aureus is an important biomarker for S. aureus. Thus, its rapid and specific detection may facilitate the pathogen identification and initiation of proper treatment. Herein, we present a simple, label-free and rapid optical biosensor enabling specific detection of protein A. Protein A-binding aptamer serves as the capture probe and is immobilized onto a nanostructured porous silicon thin film, which serves as the optical transducer element. We demonstrate high sensitivity of the biosensor with a linear detection range between 8 and 23 μM. The apparent dissociation constant was determined as 13.98 μM and the LoD is 3.17 μM. Harnessing the affinity between protein A and antibodies, a sandwich assay format was developed to amplify the optical signal associated with protein A capture by the aptamer. Using this approach, we increase the sensitivity of the biosensor, resulting in a three times lower LoD.
Keywords
- Aptamer, Label-free, Optical biosensor, Porous silicon, Protein A, Staphylococcus aureus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 257, 25.01.2017, p. 171-177.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Rapid and label-free detection of protein a by aptamer-tethered porous silicon nanostructures
AU - Urmann, Katharina
AU - Reich, Peggy
AU - Walter, Johanna Gabriela
AU - Beckmann, Dieter
AU - Segal, Ester
AU - Scheper, Thomas
N1 - Funding information: This work was funded by the German Research Foundation under the grant SCHE 279/32-1. ES acknowledges the support by the Israel Science Foundation (Grant 1146/12). ES and KU acknowledge the core services and support from the Lorry I. Lokey Center for Life Science and Engineering. PR acknowledges the financial support of the Life Science Network stipend, administered by the German Technion Society.
PY - 2017/1/25
Y1 - 2017/1/25
N2 - Protein A, which is secreted by and displayed on the cell membrane of Staphylococcus aureus is an important biomarker for S. aureus. Thus, its rapid and specific detection may facilitate the pathogen identification and initiation of proper treatment. Herein, we present a simple, label-free and rapid optical biosensor enabling specific detection of protein A. Protein A-binding aptamer serves as the capture probe and is immobilized onto a nanostructured porous silicon thin film, which serves as the optical transducer element. We demonstrate high sensitivity of the biosensor with a linear detection range between 8 and 23 μM. The apparent dissociation constant was determined as 13.98 μM and the LoD is 3.17 μM. Harnessing the affinity between protein A and antibodies, a sandwich assay format was developed to amplify the optical signal associated with protein A capture by the aptamer. Using this approach, we increase the sensitivity of the biosensor, resulting in a three times lower LoD.
AB - Protein A, which is secreted by and displayed on the cell membrane of Staphylococcus aureus is an important biomarker for S. aureus. Thus, its rapid and specific detection may facilitate the pathogen identification and initiation of proper treatment. Herein, we present a simple, label-free and rapid optical biosensor enabling specific detection of protein A. Protein A-binding aptamer serves as the capture probe and is immobilized onto a nanostructured porous silicon thin film, which serves as the optical transducer element. We demonstrate high sensitivity of the biosensor with a linear detection range between 8 and 23 μM. The apparent dissociation constant was determined as 13.98 μM and the LoD is 3.17 μM. Harnessing the affinity between protein A and antibodies, a sandwich assay format was developed to amplify the optical signal associated with protein A capture by the aptamer. Using this approach, we increase the sensitivity of the biosensor, resulting in a three times lower LoD.
KW - Aptamer
KW - Label-free
KW - Optical biosensor
KW - Porous silicon
KW - Protein A
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85013057577&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2017.01.005
DO - 10.1016/j.jbiotec.2017.01.005
M3 - Article
C2 - 28131857
AN - SCOPUS:85013057577
VL - 257
SP - 171
EP - 177
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
ER -