Details
| Original language | English |
|---|---|
| Pages (from-to) | 1307-1315 |
| Number of pages | 9 |
| Journal | Journal of the Marine Biological Association of the United Kingdom |
| Volume | 97 |
| Issue number | 6 |
| Publication status | Published - 1 Sept 2017 |
Abstract
In India 14 seagrass species can be found with monospecific genera (Enhalus, Thalassia and Syringodium), Cymodocea with two species and Halophila and Halodule represented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of the Halophila and Halodule complex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.
Keywords
- genetic variability, India, Marine angiosperm, RAPD
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Aquatic Science
Sustainable Development Goals
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In: Journal of the Marine Biological Association of the United Kingdom, Vol. 97, No. 6, 01.09.2017, p. 1307-1315.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Random amplified polymorphic DNA (RAPD) finger prints evidencing high genetic variability among marine angiosperms of India
AU - Dilipan, Elangovan
AU - Papenbrock, Jutta
AU - Thangaradjou, Thirunavakkarasu
PY - 2017/9/1
Y1 - 2017/9/1
N2 - In India 14 seagrass species can be found with monospecific genera (Enhalus, Thalassia and Syringodium), Cymodocea with two species and Halophila and Halodule represented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of the Halophila and Halodule complex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.
AB - In India 14 seagrass species can be found with monospecific genera (Enhalus, Thalassia and Syringodium), Cymodocea with two species and Halophila and Halodule represented by more than two taxonomically complex species. Considering this, the present study was made to understand the level and pattern of genetic variability among these species collected from Tamilnadu coast, India. Random amplified polymorphic DNA (RAPD) analysis was used to evaluate the level of polymorphism existing between the species. Out of the 12 primers tested, 10 primers amplified 415 DNA fragments with an average of 41.5 fragments per primer. Of the total 415 amplified fragments only 123 (29.7%) were monomorphic and the remaining 292 (70.3%) were polymorphic for Indian seagrass species. Among the 10 primers used four are identified as the key primers capable of distinguishing all the Indian seagrasses with a high degree of polymorphism and bringing representative polymorphic alleles in all the tested seagrasses. From the present investigation, this study shows that the RAPD marker technique can be used not only as a tool to analyse genetic diversity but also to resolve the taxonomic uncertainties existing in the Indian seagrasses. The efficiency of these primers in bringing out the genetic polymorphism or homogeneity among different populations of the Halophila and Halodule complex still has to be tested before recommending these primers as an identification tool for Indian seagrasses.
KW - genetic variability
KW - India
KW - Marine angiosperm
KW - RAPD
UR - http://www.scopus.com/inward/record.url?scp=84969862422&partnerID=8YFLogxK
U2 - 10.1017/S0025315416000631
DO - 10.1017/S0025315416000631
M3 - Article
AN - SCOPUS:84969862422
VL - 97
SP - 1307
EP - 1315
JO - Journal of the Marine Biological Association of the United Kingdom
JF - Journal of the Marine Biological Association of the United Kingdom
SN - 0025-3154
IS - 6
ER -