Details
| Original language | English |
|---|---|
| Title of host publication | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI |
| Editors | Attila Tarnok, Jessica P. Houston |
| Publisher | SPIE |
| ISBN (electronic) | 9781510658714 |
| Publication status | Published - 15 Mar 2023 |
| Event | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023 - San Francisco, United States Duration: 30 Jan 2023 → 1 Feb 2023 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 12383 |
| ISSN (Print) | 1605-7422 |
Abstract
Algal blooms appear most often during the summertime in marine or fresh water resources and can be harmful to humans, animals, and aquatic life. Especially cyanobacteria blooms can be dangerous as they are able to produce cyanotoxins, which can be harmful already at low concentrations. Consequently, it is very important to analyze algae and cyanobacteria blooms quickly, in situ and without complex sample preparation to identify whether the bloom can be harmful or not and to initiate further steps in time. Raman spectroscopy is capable of analyzing organic samples in situ and non-invasively with high specificity. It allows to investigate the structure of algae and cyanobacteria without physical contact and a time effective sample preparation is possible. In this work, we present a Raman based approach to analyze algae and cyanobacteria in the visible wavelength range. We determine components and the structure of the cells to distinguish potentially harmful species and also non-harmful species. Most of the fluorescence signal from the algae is suppressed so that the Raman signals from components inside the cell can be measured. The acquired spectra are processed to compare them and to retrieve information about the differences in the inner structure. Our Raman-based approach is thus suited for fast analysis and distinction of potentially harmful algae and cyanobacteria.
Keywords
- cyanobacteria, harmful algal blooms, Raman spectroscopy
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Sustainable Development Goals
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI. ed. / Attila Tarnok; Jessica P. Houston. SPIE, 2023. 123830C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12383).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Raman-based analysis and structural differentiation of potentially harmful algae and cyanobacteria
AU - Wetzel, Christoph
AU - Roth, Bernhard
N1 - Funding Information: The authors thank the German Federal Ministry of Education and Research (BMBF) for funding the research and the project CyBER (grant ref. 13N15259). We also acknowledge funding by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453).
PY - 2023/3/15
Y1 - 2023/3/15
N2 - Algal blooms appear most often during the summertime in marine or fresh water resources and can be harmful to humans, animals, and aquatic life. Especially cyanobacteria blooms can be dangerous as they are able to produce cyanotoxins, which can be harmful already at low concentrations. Consequently, it is very important to analyze algae and cyanobacteria blooms quickly, in situ and without complex sample preparation to identify whether the bloom can be harmful or not and to initiate further steps in time. Raman spectroscopy is capable of analyzing organic samples in situ and non-invasively with high specificity. It allows to investigate the structure of algae and cyanobacteria without physical contact and a time effective sample preparation is possible. In this work, we present a Raman based approach to analyze algae and cyanobacteria in the visible wavelength range. We determine components and the structure of the cells to distinguish potentially harmful species and also non-harmful species. Most of the fluorescence signal from the algae is suppressed so that the Raman signals from components inside the cell can be measured. The acquired spectra are processed to compare them and to retrieve information about the differences in the inner structure. Our Raman-based approach is thus suited for fast analysis and distinction of potentially harmful algae and cyanobacteria.
AB - Algal blooms appear most often during the summertime in marine or fresh water resources and can be harmful to humans, animals, and aquatic life. Especially cyanobacteria blooms can be dangerous as they are able to produce cyanotoxins, which can be harmful already at low concentrations. Consequently, it is very important to analyze algae and cyanobacteria blooms quickly, in situ and without complex sample preparation to identify whether the bloom can be harmful or not and to initiate further steps in time. Raman spectroscopy is capable of analyzing organic samples in situ and non-invasively with high specificity. It allows to investigate the structure of algae and cyanobacteria without physical contact and a time effective sample preparation is possible. In this work, we present a Raman based approach to analyze algae and cyanobacteria in the visible wavelength range. We determine components and the structure of the cells to distinguish potentially harmful species and also non-harmful species. Most of the fluorescence signal from the algae is suppressed so that the Raman signals from components inside the cell can be measured. The acquired spectra are processed to compare them and to retrieve information about the differences in the inner structure. Our Raman-based approach is thus suited for fast analysis and distinction of potentially harmful algae and cyanobacteria.
KW - cyanobacteria
KW - harmful algal blooms
KW - Raman spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85159670715&partnerID=8YFLogxK
U2 - 10.1117/12.2648773
DO - 10.1117/12.2648773
M3 - Conference contribution
AN - SCOPUS:85159670715
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI
A2 - Tarnok, Attila
A2 - Houston, Jessica P.
PB - SPIE
T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XXI 2023
Y2 - 30 January 2023 through 1 February 2023
ER -