Details
| Original language | English |
|---|---|
| Article number | 5349 |
| Journal | Cancers |
| Volume | 14 |
| Issue number | 21 |
| Publication status | Published - 30 Oct 2022 |
Abstract
Non-temperature-induced effects of radiofrequency electromagnetic fields (RF) have been controversial for decades. Here, we established measurement techniques to prove their existence by investigating energy deposition in tumor cells under RF exposure and upon adding amplitude modulation (AM) (AMRF). Using a preclinical device LabEHY-200 with a novel in vitro applicator, we analyzed the power deposition and system parameters for five human colorectal cancer cell lines and measured the apoptosis rates in vitro and tumor growth inhibition in vivo in comparison to water bath heating. We showed enhanced anticancer effects of RF and AMRF in vitro and in vivo and verified the non-temperature-induced origin of the effects. Furthermore, apoptotic enhancement by AM was correlated with cell membrane stiffness. Our findings not only provide a strategy to significantly enhance non-temperature-induced anticancer cell effects in vitro and in vivo but also provide a perspective for a potentially more effective tumor therapy.
Keywords
- amplitude modulation, anticancer effects, colorectal cancer, hyperthermia, radiofrequency
ASJC Scopus subject areas
- Medicine(all)
- Oncology
- Biochemistry, Genetics and Molecular Biology(all)
- Cancer Research
Sustainable Development Goals
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In: Cancers, Vol. 14, No. 21, 5349, 30.10.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Radiofrequency Electromagnetic Fields Cause Non-Temperature-Induced Physical and Biological Effects in Cancer Cells
AU - Wust, Peter
AU - Veltsista, Paraskevi D
AU - Oberacker, Eva
AU - Yavvari, Prabhusrinivas
AU - Walther, Wolfgang
AU - Bengtsson, Olof
AU - Sterner-Kock, Anja
AU - Weinhart, Marie
AU - Heyd, Florian
AU - Grabowski, Patricia
AU - Stintzing, Sebastian
AU - Heinrich, Wolfgang
AU - Stein, Ulrike
AU - Ghadjar, Pirus
N1 - Funding Information: This research was funded by the Berliner Krebsgesellschaft e.V., grant number GHFF202006, www.berliner-krebsgesellschaft.de. This project was supported by the H2020 Marie Sklodowska–Curie program—HYPERBOOST (project number 955625).
PY - 2022/10/30
Y1 - 2022/10/30
N2 - Non-temperature-induced effects of radiofrequency electromagnetic fields (RF) have been controversial for decades. Here, we established measurement techniques to prove their existence by investigating energy deposition in tumor cells under RF exposure and upon adding amplitude modulation (AM) (AMRF). Using a preclinical device LabEHY-200 with a novel in vitro applicator, we analyzed the power deposition and system parameters for five human colorectal cancer cell lines and measured the apoptosis rates in vitro and tumor growth inhibition in vivo in comparison to water bath heating. We showed enhanced anticancer effects of RF and AMRF in vitro and in vivo and verified the non-temperature-induced origin of the effects. Furthermore, apoptotic enhancement by AM was correlated with cell membrane stiffness. Our findings not only provide a strategy to significantly enhance non-temperature-induced anticancer cell effects in vitro and in vivo but also provide a perspective for a potentially more effective tumor therapy.
AB - Non-temperature-induced effects of radiofrequency electromagnetic fields (RF) have been controversial for decades. Here, we established measurement techniques to prove their existence by investigating energy deposition in tumor cells under RF exposure and upon adding amplitude modulation (AM) (AMRF). Using a preclinical device LabEHY-200 with a novel in vitro applicator, we analyzed the power deposition and system parameters for five human colorectal cancer cell lines and measured the apoptosis rates in vitro and tumor growth inhibition in vivo in comparison to water bath heating. We showed enhanced anticancer effects of RF and AMRF in vitro and in vivo and verified the non-temperature-induced origin of the effects. Furthermore, apoptotic enhancement by AM was correlated with cell membrane stiffness. Our findings not only provide a strategy to significantly enhance non-temperature-induced anticancer cell effects in vitro and in vivo but also provide a perspective for a potentially more effective tumor therapy.
KW - amplitude modulation
KW - anticancer effects
KW - colorectal cancer
KW - hyperthermia
KW - radiofrequency
UR - http://www.scopus.com/inward/record.url?scp=85141873280&partnerID=8YFLogxK
U2 - 10.3390/cancers14215349
DO - 10.3390/cancers14215349
M3 - Article
C2 - 36358768
VL - 14
JO - Cancers
JF - Cancers
SN - 2072-6694
IS - 21
M1 - 5349
ER -