Details
| Original language | English |
|---|---|
| Pages (from-to) | 1-6 |
| Number of pages | 6 |
| Journal | Biomedical Engineering - Applications, Basis and Communications |
| Volume | 16 |
| Issue number | 1 |
| Publication status | Published - 25 Feb 2004 |
| Externally published | Yes |
Abstract
In applied transgenic research as well as in agriculture there is an increasing need for high-throughput analyses of plants for genotypic selection or to identify the purity of seed stocks, e.g. for transgenic contaminations or the identification of pathogens. We developed and optimised conditions for the isolation of DNA from single seeds using an automated high-throughput protocol. Our results show that the system provided is capable of isolating DNA from any tested seed source. Furthermore, seeds remain capable of germinating during the homogenisation procedure. Quantification of endogenous and transgenic sequences by Real-Time PCR revealed that the prepared DNA is suitable in quality and quantity for multiple PCR analyses with both SYBR Green and hybridisation probe detection. The described method will be a useful tool for routine analyses like screening of large populations or the specific detection of genetically modified organisms (GMO).
Keywords
- Automation, DNA isolation, Magnapure, Real-Time PCR, Seeds
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
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In: Biomedical Engineering - Applications, Basis and Communications, Vol. 16, No. 1, 25.02.2004, p. 1-6.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Quantitative detection of transgenic and endogenous DNA sequences in seeds after automated DNA preparation
AU - Peterhänsel, Christoph
AU - Hahnen, Silke
AU - Kalamajka, Rainer
AU - Offermann, Sascha
AU - Miedl, Brigitte
AU - Rüger, Barbara
PY - 2004/2/25
Y1 - 2004/2/25
N2 - In applied transgenic research as well as in agriculture there is an increasing need for high-throughput analyses of plants for genotypic selection or to identify the purity of seed stocks, e.g. for transgenic contaminations or the identification of pathogens. We developed and optimised conditions for the isolation of DNA from single seeds using an automated high-throughput protocol. Our results show that the system provided is capable of isolating DNA from any tested seed source. Furthermore, seeds remain capable of germinating during the homogenisation procedure. Quantification of endogenous and transgenic sequences by Real-Time PCR revealed that the prepared DNA is suitable in quality and quantity for multiple PCR analyses with both SYBR Green and hybridisation probe detection. The described method will be a useful tool for routine analyses like screening of large populations or the specific detection of genetically modified organisms (GMO).
AB - In applied transgenic research as well as in agriculture there is an increasing need for high-throughput analyses of plants for genotypic selection or to identify the purity of seed stocks, e.g. for transgenic contaminations or the identification of pathogens. We developed and optimised conditions for the isolation of DNA from single seeds using an automated high-throughput protocol. Our results show that the system provided is capable of isolating DNA from any tested seed source. Furthermore, seeds remain capable of germinating during the homogenisation procedure. Quantification of endogenous and transgenic sequences by Real-Time PCR revealed that the prepared DNA is suitable in quality and quantity for multiple PCR analyses with both SYBR Green and hybridisation probe detection. The described method will be a useful tool for routine analyses like screening of large populations or the specific detection of genetically modified organisms (GMO).
KW - Automation
KW - DNA isolation
KW - Magnapure
KW - Real-Time PCR
KW - Seeds
UR - http://www.scopus.com/inward/record.url?scp=1542288247&partnerID=8YFLogxK
U2 - 10.4015/S1016237204000025
DO - 10.4015/S1016237204000025
M3 - Article
AN - SCOPUS:1542288247
VL - 16
SP - 1
EP - 6
JO - Biomedical Engineering - Applications, Basis and Communications
JF - Biomedical Engineering - Applications, Basis and Communications
SN - 1016-2372
IS - 1
ER -