Details
| Original language | English |
|---|---|
| Title of host publication | Three-Dimensional and Multidimensional Microscopy |
| Subtitle of host publication | Image Acquisition and Processing XXIX |
| Editors | Thomas G. Brown, Tony Wilson, Laura Waller |
| Publisher | SPIE |
| ISBN (electronic) | 9781510648036 |
| Publication status | Published - 2 Mar 2022 |
| Event | Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022 - Virtual, Online Duration: 20 Feb 2022 → 24 Feb 2022 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 11966 |
| ISSN (Print) | 1605-7422 |
Abstract
The triple-negative breast cancer (tnbc) is an aggressive subtype linked to a poor outcome of established breast cancer therapy. Increasing evidence points to the role of the tumor's extracellular matrix (ECM) as a determinant of its aggressiveness as well as the effectiveness of chemical therapeutics. Three-dimensional imaging techniques can be used to unravel ECM architecture. Label-free contrast mechanisms such as second harmonic generation (SHG) avoid falsification and artifacts introduced by the labeling process. Here, we present the complementary use of two-photon excitation microscopy (TPEF) and Scanning Laser Optical Tomography (SLOT) for the investigation and quantification of tumor ECM. Both methods were used to capture fluorescence from antibody-labeled samples as well as the SHG signal from collagen strands in the ECM. SLOT generally allows for the investigation of larger samples of several mm up to a few cm in size. This work shows the capabilities of the tomographic setup compared to established TPEF, and demonstrates their combined use to maximize the information content of the acquired data. The obtained images served as a basis for ECM quantification. 3D-analysis allowed for determination of length, straightness and orientation of the collagen fibers based on fluorescence imaging as well as SHG imaging. The resulting coordinates might be used for synthetic reconstruction of a patient-specific tumor matrix, serving as a scaffold for pre-clinical therapeutic testing. Collagen imaging and quantification as presented here can therefore be employed for both basic and clinical research, paving the way for patient-specific cancer therapy.
Keywords
- 3D imaging, Cancer, collagen imaging, extracellular matrix, image reconstruction, multimodal imaging, second harmonic generation, tomography
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Sustainable Development Goals
Cite this
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- Apa
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- BibTeX
- RIS
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX. ed. / Thomas G. Brown; Tony Wilson; Laura Waller. SPIE, 2022. 119660B (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11966).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Quantification of collagen networks in mammary tumors using TPEF and laser-based tomography
AU - Kamin, Hannes
AU - Nolte, Lena
AU - Maurer, Jochen
AU - Bleilevens, Andreas
AU - Stickeler, Elmar
AU - Johannsmeier, Sonja
AU - Heinemann, Dag
AU - Ripken, Tammo
N1 - Funding Information: This work is funded by the Federal Ministry of Education and Research, Germany, Grant no. 031L0146C
PY - 2022/3/2
Y1 - 2022/3/2
N2 - The triple-negative breast cancer (tnbc) is an aggressive subtype linked to a poor outcome of established breast cancer therapy. Increasing evidence points to the role of the tumor's extracellular matrix (ECM) as a determinant of its aggressiveness as well as the effectiveness of chemical therapeutics. Three-dimensional imaging techniques can be used to unravel ECM architecture. Label-free contrast mechanisms such as second harmonic generation (SHG) avoid falsification and artifacts introduced by the labeling process. Here, we present the complementary use of two-photon excitation microscopy (TPEF) and Scanning Laser Optical Tomography (SLOT) for the investigation and quantification of tumor ECM. Both methods were used to capture fluorescence from antibody-labeled samples as well as the SHG signal from collagen strands in the ECM. SLOT generally allows for the investigation of larger samples of several mm up to a few cm in size. This work shows the capabilities of the tomographic setup compared to established TPEF, and demonstrates their combined use to maximize the information content of the acquired data. The obtained images served as a basis for ECM quantification. 3D-analysis allowed for determination of length, straightness and orientation of the collagen fibers based on fluorescence imaging as well as SHG imaging. The resulting coordinates might be used for synthetic reconstruction of a patient-specific tumor matrix, serving as a scaffold for pre-clinical therapeutic testing. Collagen imaging and quantification as presented here can therefore be employed for both basic and clinical research, paving the way for patient-specific cancer therapy.
AB - The triple-negative breast cancer (tnbc) is an aggressive subtype linked to a poor outcome of established breast cancer therapy. Increasing evidence points to the role of the tumor's extracellular matrix (ECM) as a determinant of its aggressiveness as well as the effectiveness of chemical therapeutics. Three-dimensional imaging techniques can be used to unravel ECM architecture. Label-free contrast mechanisms such as second harmonic generation (SHG) avoid falsification and artifacts introduced by the labeling process. Here, we present the complementary use of two-photon excitation microscopy (TPEF) and Scanning Laser Optical Tomography (SLOT) for the investigation and quantification of tumor ECM. Both methods were used to capture fluorescence from antibody-labeled samples as well as the SHG signal from collagen strands in the ECM. SLOT generally allows for the investigation of larger samples of several mm up to a few cm in size. This work shows the capabilities of the tomographic setup compared to established TPEF, and demonstrates their combined use to maximize the information content of the acquired data. The obtained images served as a basis for ECM quantification. 3D-analysis allowed for determination of length, straightness and orientation of the collagen fibers based on fluorescence imaging as well as SHG imaging. The resulting coordinates might be used for synthetic reconstruction of a patient-specific tumor matrix, serving as a scaffold for pre-clinical therapeutic testing. Collagen imaging and quantification as presented here can therefore be employed for both basic and clinical research, paving the way for patient-specific cancer therapy.
KW - 3D imaging
KW - Cancer
KW - collagen imaging
KW - extracellular matrix
KW - image reconstruction
KW - multimodal imaging
KW - second harmonic generation
KW - tomography
UR - http://www.scopus.com/inward/record.url?scp=85131208368&partnerID=8YFLogxK
U2 - 10.1117/12.2609425
DO - 10.1117/12.2609425
M3 - Conference contribution
AN - SCOPUS:85131208368
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Three-Dimensional and Multidimensional Microscopy
A2 - Brown, Thomas G.
A2 - Wilson, Tony
A2 - Waller, Laura
PB - SPIE
T2 - Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX 2022
Y2 - 20 February 2022 through 24 February 2022
ER -