Details
| Original language | English |
|---|---|
| Article number | 11373 |
| Journal | International Journal of Molecular Sciences |
| Volume | 22 |
| Issue number | 21 |
| Publication status | Published - 21 Oct 2021 |
Abstract
Polyvinylidene fluoride (PVDF) and its copolymer with trifluoroethylene (P(VDF-TrFE)) are considered as promising biomaterials for supporting nerve regeneration because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to their electrical activity upon mechanical deformation. For the first time, this study reports on the comparative analysis of PVDF and P(VDF-TrFE) electrospun scaffolds in terms of structural and piezoelectric properties as well as their in vitro performance. A dynamic impact test machine was developed, validated, and utilised, to evaluate the generation of an electrical voltage upon the application of an impact load (varying load magnitude and frequency) onto the electrospun PVDF (15–20 wt%) and P(VDF-TrFE) (10–20 wt%) scaffolds. The cytotoxicity and in vitro performance of the scaffolds was evaluated with neonatal rat (nrSCs) and adult human Schwann cells (ahSCs). The neurite outgrowth behaviour from sensory rat dorsal root ganglion neurons cultured on the scaffolds was analysed qualitatively. The results showed (i) a significant increase of the β-phase content in the PVDF after electrospinning as well as a zeta potential similar to P(VDF-TrFE), (ii) a non-constant behaviour of the longitudinal piezoelectric strain constant d33, depending on the load and the load frequency, and (iii) biocompatibility with cultured Schwann cells and guiding properties for sensory neurite outgrowth. In summary, the electrospun PVDF-based scaffolds, representing piezoelectric activity, can be considered as promising materials for the development of artificial nerve conduits for the peripheral nerve injury repair.
Keywords
- Dynamic impact machine, Electrospinning, In vitro performance, Nerve conduit, Neurite outgrowth, Peripheral nervous system, Piezoelectric module, Polyvinylidene fluoride, Polyvinylidene fluoride-co-trifluoroethylene, Scaffold, Zeta potential
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Spectroscopy
- Computer Science(all)
- Computer Science Applications
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemistry(all)
- Organic Chemistry
- Chemistry(all)
- Inorganic Chemistry
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In: International Journal of Molecular Sciences, Vol. 22, No. 21, 11373, 21.10.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Pvdf and p(Vdf-trfe) electrospun scaffolds for nerve graft engineering
T2 - A comparative study on piezoelectric and structural properties, and in vitro biocompatibility
AU - Gryshkov, Oleksandr
AU - Al Halabi, Fedaa
AU - Kuhn, Antonia Isabel
AU - Leal-Marin, Sara
AU - Freund, Lena Julie
AU - Förthmann, Maria
AU - Meier, Nils
AU - Barker, Sven Alexander
AU - Haastert-Talini, Kirsten
AU - Glasmacher, Birgit
N1 - Funding Information: This work has in part been supported by the International Neurobionics Foundation, Dr. Heinz Lindemann Foundation, Victor-Rizkallah-Foundation as well as Deutsche Forschungsgemeinschaft. The publication of this article was funded by the Open Access Fund of Leibniz University Hannover.The authors are grateful to Henning Menzel (Institute for Technical Chemistry, Braunschweig University of Technology) for providing a device for zeta potential measurements. Authors express their gratitude to Igor Katz, Julia Guewa, Katerina Zelena, Silvana Taubeler-Gerling, Jennifer Metzen, and Maike Wesemann for their excellent technical assistance. We thank Klaus E. Goehrmann and the International Neurobionics Foundation board for the partial financial support of this study.
PY - 2021/10/21
Y1 - 2021/10/21
N2 - Polyvinylidene fluoride (PVDF) and its copolymer with trifluoroethylene (P(VDF-TrFE)) are considered as promising biomaterials for supporting nerve regeneration because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to their electrical activity upon mechanical deformation. For the first time, this study reports on the comparative analysis of PVDF and P(VDF-TrFE) electrospun scaffolds in terms of structural and piezoelectric properties as well as their in vitro performance. A dynamic impact test machine was developed, validated, and utilised, to evaluate the generation of an electrical voltage upon the application of an impact load (varying load magnitude and frequency) onto the electrospun PVDF (15–20 wt%) and P(VDF-TrFE) (10–20 wt%) scaffolds. The cytotoxicity and in vitro performance of the scaffolds was evaluated with neonatal rat (nrSCs) and adult human Schwann cells (ahSCs). The neurite outgrowth behaviour from sensory rat dorsal root ganglion neurons cultured on the scaffolds was analysed qualitatively. The results showed (i) a significant increase of the β-phase content in the PVDF after electrospinning as well as a zeta potential similar to P(VDF-TrFE), (ii) a non-constant behaviour of the longitudinal piezoelectric strain constant d33, depending on the load and the load frequency, and (iii) biocompatibility with cultured Schwann cells and guiding properties for sensory neurite outgrowth. In summary, the electrospun PVDF-based scaffolds, representing piezoelectric activity, can be considered as promising materials for the development of artificial nerve conduits for the peripheral nerve injury repair.
AB - Polyvinylidene fluoride (PVDF) and its copolymer with trifluoroethylene (P(VDF-TrFE)) are considered as promising biomaterials for supporting nerve regeneration because of their proven biocompatibility and piezoelectric properties that could stimulate cell ingrowth due to their electrical activity upon mechanical deformation. For the first time, this study reports on the comparative analysis of PVDF and P(VDF-TrFE) electrospun scaffolds in terms of structural and piezoelectric properties as well as their in vitro performance. A dynamic impact test machine was developed, validated, and utilised, to evaluate the generation of an electrical voltage upon the application of an impact load (varying load magnitude and frequency) onto the electrospun PVDF (15–20 wt%) and P(VDF-TrFE) (10–20 wt%) scaffolds. The cytotoxicity and in vitro performance of the scaffolds was evaluated with neonatal rat (nrSCs) and adult human Schwann cells (ahSCs). The neurite outgrowth behaviour from sensory rat dorsal root ganglion neurons cultured on the scaffolds was analysed qualitatively. The results showed (i) a significant increase of the β-phase content in the PVDF after electrospinning as well as a zeta potential similar to P(VDF-TrFE), (ii) a non-constant behaviour of the longitudinal piezoelectric strain constant d33, depending on the load and the load frequency, and (iii) biocompatibility with cultured Schwann cells and guiding properties for sensory neurite outgrowth. In summary, the electrospun PVDF-based scaffolds, representing piezoelectric activity, can be considered as promising materials for the development of artificial nerve conduits for the peripheral nerve injury repair.
KW - Dynamic impact machine
KW - Electrospinning
KW - In vitro performance
KW - Nerve conduit
KW - Neurite outgrowth
KW - Peripheral nervous system
KW - Piezoelectric module
KW - Polyvinylidene fluoride
KW - Polyvinylidene fluoride-co-trifluoroethylene
KW - Scaffold
KW - Zeta potential
UR - http://www.scopus.com/inward/record.url?scp=85117389050&partnerID=8YFLogxK
U2 - 10.3390/ijms222111373
DO - 10.3390/ijms222111373
M3 - Article
C2 - 34768804
AN - SCOPUS:85117389050
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 21
M1 - 11373
ER -