Details
| Original language | English |
|---|---|
| Pages (from-to) | 693-700 |
| Number of pages | 8 |
| Journal | Journal of biotechnology |
| Volume | 168 |
| Issue number | 4 |
| Publication status | Published - 30 Sept 2013 |
Abstract
Potato fruit juice (PFJ) is a by-product from industrial starch production. It still contains several valuable components such as amino acids, minerals and proteins. An economic technology for the isolation and purification of different native potato proteins is the ion exchange chromatography, which can be performed either by classical bed chromatography or by membrane adsorption chromatography (MA-IEX). An already published MA-IEX process for the downstreaming of PFJ is based on the following steps: prefiltration/microfiltration, fractionation with MA-IEX, ultra-/diafiltration and finally drying. In order to further minimize process complexity and costs, new MA-IEX-modules were designed and tested in this research project to facilitate the processing of crude, particle-containing solutions using a tangential flow through the membranes. Modules with fleece polymer spacers and extruded polymer spacers, as well as different spacer channel sizes were tested for their binding capacities and their long-term stability. An optimized setup was found for the technical scale. Modules with extruded polymer spacers channel size 250μm show the highest binding capacities (anion exchanger approx. 0.34mg/cm2, cation exchanger approx. 0.16mg/cm2), while the modules with extruded polymer spacers channel size 480μm show the best long-term stability with 23 passes without intermediary cleaning.
Keywords
- Binding capacity, Long-term stability, Membrane adsorber, Protein, Purification
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 168, No. 4, 30.09.2013, p. 693-700.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Purification of high value proteins from particle containing potato fruit juice via direct capture membrane adsorption chromatography
AU - Schoenbeck, I.
AU - Graf, A. M.
AU - Leuthold, M.
AU - Pastor, A.
AU - Beutel, S.
AU - Scheper, T.
N1 - Funding information: We thank the Lower Saxony Ministry of Science and Culture for the financial support within the frame of the Research alliance Nutritional Sciences Lower Saxony.
PY - 2013/9/30
Y1 - 2013/9/30
N2 - Potato fruit juice (PFJ) is a by-product from industrial starch production. It still contains several valuable components such as amino acids, minerals and proteins. An economic technology for the isolation and purification of different native potato proteins is the ion exchange chromatography, which can be performed either by classical bed chromatography or by membrane adsorption chromatography (MA-IEX). An already published MA-IEX process for the downstreaming of PFJ is based on the following steps: prefiltration/microfiltration, fractionation with MA-IEX, ultra-/diafiltration and finally drying. In order to further minimize process complexity and costs, new MA-IEX-modules were designed and tested in this research project to facilitate the processing of crude, particle-containing solutions using a tangential flow through the membranes. Modules with fleece polymer spacers and extruded polymer spacers, as well as different spacer channel sizes were tested for their binding capacities and their long-term stability. An optimized setup was found for the technical scale. Modules with extruded polymer spacers channel size 250μm show the highest binding capacities (anion exchanger approx. 0.34mg/cm2, cation exchanger approx. 0.16mg/cm2), while the modules with extruded polymer spacers channel size 480μm show the best long-term stability with 23 passes without intermediary cleaning.
AB - Potato fruit juice (PFJ) is a by-product from industrial starch production. It still contains several valuable components such as amino acids, minerals and proteins. An economic technology for the isolation and purification of different native potato proteins is the ion exchange chromatography, which can be performed either by classical bed chromatography or by membrane adsorption chromatography (MA-IEX). An already published MA-IEX process for the downstreaming of PFJ is based on the following steps: prefiltration/microfiltration, fractionation with MA-IEX, ultra-/diafiltration and finally drying. In order to further minimize process complexity and costs, new MA-IEX-modules were designed and tested in this research project to facilitate the processing of crude, particle-containing solutions using a tangential flow through the membranes. Modules with fleece polymer spacers and extruded polymer spacers, as well as different spacer channel sizes were tested for their binding capacities and their long-term stability. An optimized setup was found for the technical scale. Modules with extruded polymer spacers channel size 250μm show the highest binding capacities (anion exchanger approx. 0.34mg/cm2, cation exchanger approx. 0.16mg/cm2), while the modules with extruded polymer spacers channel size 480μm show the best long-term stability with 23 passes without intermediary cleaning.
KW - Binding capacity
KW - Long-term stability
KW - Membrane adsorber
KW - Protein
KW - Purification
UR - http://www.scopus.com/inward/record.url?scp=84888840060&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2013.09.018
DO - 10.1016/j.jbiotec.2013.09.018
M3 - Article
C2 - 24091299
AN - SCOPUS:84888840060
VL - 168
SP - 693
EP - 700
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 4
ER -