Details
| Original language | English |
|---|---|
| Pages (from-to) | 128-132 |
| Number of pages | 5 |
| Journal | FEBS letters |
| Volume | 316 |
| Issue number | 2 |
| Publication status | Published - 25 Jan 1993 |
| Externally published | Yes |
Abstract
Several mitochondrial genes from a large number of different fungi, mammals and plants have been sequenced but little is known about the corresponding translation products. We have affinity purified cytochrome c reductase from potato mitochondria and isolated the mitochondrially encoded cytochrome b protein. Amino-terminal sequencing reveals that the polypeptide does not start with a methionine. Comparison of the amino acid sequence with the recently published sequence of the gene encoding the cytochrome b apoprotein suggests that the N-fonnylmetnionine is removed. This result provides the first evidence for the presence of a deformylase and a methionine aminopeptidase in mitochondria.
Keywords
- Cytochrome b, Cytochrome c reductase, Methionine aminopeptidase, Mitochondria, Solanum tuberosum
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Structural Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: FEBS letters, Vol. 316, No. 2, 25.01.1993, p. 128-132.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Purification and sequencing of cytochrome b from potato reveals methionine cleavage of a mitochondriallly encoded protein
AU - Braun, Hans-Peter
AU - Schmitz, Udo
N1 - Funding information: AcknowledgemenWtse: are greatlyi ndeptedt o Dr. V. Kruft, Berlin, for sequencingth e cytochromeb protein.W e wish to thankP rof. G. Schatz,B asel,w ho madea ntibodiesa gainstc ytochromebs from yeast available to us. This work was supported by the Deutsche Forschungsgemeinscha(Sftc hm6 98/2-2).
PY - 1993/1/25
Y1 - 1993/1/25
N2 - Several mitochondrial genes from a large number of different fungi, mammals and plants have been sequenced but little is known about the corresponding translation products. We have affinity purified cytochrome c reductase from potato mitochondria and isolated the mitochondrially encoded cytochrome b protein. Amino-terminal sequencing reveals that the polypeptide does not start with a methionine. Comparison of the amino acid sequence with the recently published sequence of the gene encoding the cytochrome b apoprotein suggests that the N-fonnylmetnionine is removed. This result provides the first evidence for the presence of a deformylase and a methionine aminopeptidase in mitochondria.
AB - Several mitochondrial genes from a large number of different fungi, mammals and plants have been sequenced but little is known about the corresponding translation products. We have affinity purified cytochrome c reductase from potato mitochondria and isolated the mitochondrially encoded cytochrome b protein. Amino-terminal sequencing reveals that the polypeptide does not start with a methionine. Comparison of the amino acid sequence with the recently published sequence of the gene encoding the cytochrome b apoprotein suggests that the N-fonnylmetnionine is removed. This result provides the first evidence for the presence of a deformylase and a methionine aminopeptidase in mitochondria.
KW - Cytochrome b
KW - Cytochrome c reductase
KW - Methionine aminopeptidase
KW - Mitochondria
KW - Solanum tuberosum
UR - http://www.scopus.com/inward/record.url?scp=0027498123&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(93)81200-J
DO - 10.1016/0014-5793(93)81200-J
M3 - Article
C2 - 8420797
AN - SCOPUS:0027498123
VL - 316
SP - 128
EP - 132
JO - FEBS letters
JF - FEBS letters
SN - 0014-5793
IS - 2
ER -