Details
| Original language | English |
|---|---|
| Title of host publication | Affinity Chromatography |
| Subtitle of host publication | Methods and Protocols |
| Pages | 145-155 |
| Number of pages | 11 |
| ISBN (electronic) | 978-1-0716-2176-9 |
| Publication status | Published - 19 May 2022 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2466 |
| ISSN (Print) | 1064-3745 |
| ISSN (electronic) | 1940-6029 |
Abstract
This protocol describes necessary steps to isolate and quantify nucleotides and nucleosides from plant samples. Proper sample preparation in combination with liquid chromatography coupled to mass spectrometry enables the sensitive detection and quantification of metabolites of low abundance. Utilizing a liquid–liquid extraction in combination with a weak anion-exchange solid phase extraction enables the separation of negatively charged molecules from uncharged metabolites or cations.
Keywords
- Liquid chromatography, Mass spectrometry, Metabolomics, Plant nucleotide metabolism, Polar metabolomics, Weak anion-exchange solid phase extraction
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
Cite this
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Affinity Chromatography: Methods and Protocols. 2022. p. 145-155 (Methods in Molecular Biology; Vol. 2466).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Purification and Analysis of Nucleotides and Nucleosides from Plants
AU - Straube, Henryk
AU - Herde, Marco
PY - 2022/5/19
Y1 - 2022/5/19
N2 - This protocol describes necessary steps to isolate and quantify nucleotides and nucleosides from plant samples. Proper sample preparation in combination with liquid chromatography coupled to mass spectrometry enables the sensitive detection and quantification of metabolites of low abundance. Utilizing a liquid–liquid extraction in combination with a weak anion-exchange solid phase extraction enables the separation of negatively charged molecules from uncharged metabolites or cations.
AB - This protocol describes necessary steps to isolate and quantify nucleotides and nucleosides from plant samples. Proper sample preparation in combination with liquid chromatography coupled to mass spectrometry enables the sensitive detection and quantification of metabolites of low abundance. Utilizing a liquid–liquid extraction in combination with a weak anion-exchange solid phase extraction enables the separation of negatively charged molecules from uncharged metabolites or cations.
KW - Liquid chromatography
KW - Mass spectrometry
KW - Metabolomics
KW - Plant nucleotide metabolism
KW - Polar metabolomics
KW - Weak anion-exchange solid phase extraction
UR - http://www.scopus.com/inward/record.url?scp=85130293934&partnerID=8YFLogxK
U2 - 10.15488/17932
DO - 10.15488/17932
M3 - Contribution to book/anthology
C2 - 35585317
AN - SCOPUS:85130293934
SN - 978-1-0716-2175-2
SN - 978-1-0716-2178-3
T3 - Methods in Molecular Biology
SP - 145
EP - 155
BT - Affinity Chromatography
ER -