Details
| Original language | English |
|---|---|
| Pages (from-to) | 703-708 |
| Number of pages | 6 |
| Journal | Biotechnology letters |
| Volume | 15 |
| Issue number | 7 |
| Publication status | Published - 1 Jul 1993 |
Abstract
The activity of purified Pseudomonas cepacia lipase has been investigated in esterification reactions of various aliphatic alcohols with natural fatty acids. The reactions were carried out in microemulsions formed in isooctane by bis(2ethylhexyl)sulfosuccinate sodium salt (AOT). The optima pH, T and water content (wo) for the enzyme activity in this type of microemulsions have been determined. Studies on the effect of various fatty acids and alcohols on the enzyme specificity have shown a preference of this lipase for palmitic and caprylic acid as well as for propanol, while reactions involving cyclic alcohols can not be catalyzed at all. The differences on the behavior of this lipase as compared to other lipases studied in microemulsion systems as well as in other systems are discussed.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Biotechnology letters, Vol. 15, No. 7, 01.07.1993, p. 703-708.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Pseudomonas cepacia lipase: Esterification reactions in AOT microemulsion systems
AU - Stamatis, H.
AU - Xenakis, A.
AU - Bornscheuer, U.
AU - Scheper, T.
AU - Menge, U.
AU - Kolisis, F. N.
PY - 1993/7/1
Y1 - 1993/7/1
N2 - The activity of purified Pseudomonas cepacia lipase has been investigated in esterification reactions of various aliphatic alcohols with natural fatty acids. The reactions were carried out in microemulsions formed in isooctane by bis(2ethylhexyl)sulfosuccinate sodium salt (AOT). The optima pH, T and water content (wo) for the enzyme activity in this type of microemulsions have been determined. Studies on the effect of various fatty acids and alcohols on the enzyme specificity have shown a preference of this lipase for palmitic and caprylic acid as well as for propanol, while reactions involving cyclic alcohols can not be catalyzed at all. The differences on the behavior of this lipase as compared to other lipases studied in microemulsion systems as well as in other systems are discussed.
AB - The activity of purified Pseudomonas cepacia lipase has been investigated in esterification reactions of various aliphatic alcohols with natural fatty acids. The reactions were carried out in microemulsions formed in isooctane by bis(2ethylhexyl)sulfosuccinate sodium salt (AOT). The optima pH, T and water content (wo) for the enzyme activity in this type of microemulsions have been determined. Studies on the effect of various fatty acids and alcohols on the enzyme specificity have shown a preference of this lipase for palmitic and caprylic acid as well as for propanol, while reactions involving cyclic alcohols can not be catalyzed at all. The differences on the behavior of this lipase as compared to other lipases studied in microemulsion systems as well as in other systems are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0027249012&partnerID=8YFLogxK
U2 - 10.1007/BF01080143
DO - 10.1007/BF01080143
M3 - Article
AN - SCOPUS:0027249012
VL - 15
SP - 703
EP - 708
JO - Biotechnology letters
JF - Biotechnology letters
SN - 0141-5492
IS - 7
ER -