Details
| Original language | English |
|---|---|
| Article number | 004799 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 71 |
| Issue number | 5 |
| Publication status | Published - 5 May 2021 |
Abstract
A novel strain was isolated from grassland soil that has the potential to assimilate ammonium by the reduction of nitrate in the presence of oxygen. Whole genome sequence analysis revealed the presence of an assimilatory cytoplasmic nitrate reductase gene nasA and the assimilatory nitrite reductase genes nirBD which are involved in the sequential reduction of nitrate to nitrite and further to ammonium, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents a member of the genus Pseudomonas. The closest phylogenetic neighbours based on 16S rRNA gene sequence analysis are the type strains of Pseudomonas peli (98.17%) and Pseudomonas guineae (98.03%). In contrast, phylogenomic analysis revealed a close relationship to Pseudomonas alcaligenes. Computation of the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbours of S1-A32-2T revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. On the basis of these results, it was concluded that the soil isolate represents a novel species of the genus Pseudomonas, for which the name Pseu-domonas campi sp. nov. (type strain S1-A32-2T=LMG 31521T=DSM 110222T) is proposed.
Keywords
- Assimilative nitrate reduction, Phylogenomic analysis, Polyphasic taxonomy, S: Pseudomonas campi sp. nov, Soil bacterium
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
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In: International Journal of Systematic and Evolutionary Microbiology, Vol. 71, No. 5, 004799, 05.05.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Pseudomonas campi sp. nov., a nitrate-reducing bacterium isolated from grassland soil
AU - Timsy,
AU - Spanner, Tobias
AU - Ulrich, Andreas
AU - Kublik, Susanne
AU - Foesel, Bärbel U.
AU - Kolb, Steffen
AU - Horn, Marcus A.
AU - Behrendt, Undine
N1 - Funding Information: The work has been funded by the DFG Priority Program 1374 ‘Infrastructure-Biodiversity-Exploratories’. The field work permit was issued by the state environmental offices of Brandenburg.
PY - 2021/5/5
Y1 - 2021/5/5
N2 - A novel strain was isolated from grassland soil that has the potential to assimilate ammonium by the reduction of nitrate in the presence of oxygen. Whole genome sequence analysis revealed the presence of an assimilatory cytoplasmic nitrate reductase gene nasA and the assimilatory nitrite reductase genes nirBD which are involved in the sequential reduction of nitrate to nitrite and further to ammonium, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents a member of the genus Pseudomonas. The closest phylogenetic neighbours based on 16S rRNA gene sequence analysis are the type strains of Pseudomonas peli (98.17%) and Pseudomonas guineae (98.03%). In contrast, phylogenomic analysis revealed a close relationship to Pseudomonas alcaligenes. Computation of the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbours of S1-A32-2T revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. On the basis of these results, it was concluded that the soil isolate represents a novel species of the genus Pseudomonas, for which the name Pseu-domonas campi sp. nov. (type strain S1-A32-2T=LMG 31521T=DSM 110222T) is proposed.
AB - A novel strain was isolated from grassland soil that has the potential to assimilate ammonium by the reduction of nitrate in the presence of oxygen. Whole genome sequence analysis revealed the presence of an assimilatory cytoplasmic nitrate reductase gene nasA and the assimilatory nitrite reductase genes nirBD which are involved in the sequential reduction of nitrate to nitrite and further to ammonium, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate represents a member of the genus Pseudomonas. The closest phylogenetic neighbours based on 16S rRNA gene sequence analysis are the type strains of Pseudomonas peli (98.17%) and Pseudomonas guineae (98.03%). In contrast, phylogenomic analysis revealed a close relationship to Pseudomonas alcaligenes. Computation of the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) with the closest phylogenetic neighbours of S1-A32-2T revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. On the basis of these results, it was concluded that the soil isolate represents a novel species of the genus Pseudomonas, for which the name Pseu-domonas campi sp. nov. (type strain S1-A32-2T=LMG 31521T=DSM 110222T) is proposed.
KW - Assimilative nitrate reduction
KW - Phylogenomic analysis
KW - Polyphasic taxonomy
KW - S: Pseudomonas campi sp. nov
KW - Soil bacterium
UR - http://www.scopus.com/inward/record.url?scp=85105189138&partnerID=8YFLogxK
U2 - 10.15488/15943
DO - 10.15488/15943
M3 - Article
AN - SCOPUS:85105189138
VL - 71
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 5
M1 - 004799
ER -