Details
| Original language | English |
|---|---|
| Pages (from-to) | 27-34 |
| Number of pages | 8 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 99 |
| Issue number | 1 |
| Publication status | Published - 8 Aug 2009 |
Abstract
Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.
Keywords
- Callus formation, Plant regeneration and stability, Protoplast yield
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 99, No. 1, 08.08.2009, p. 27-34.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Protoplast isolation and plant regeneration of different genotypes of Petunia and calibrachoa
AU - Meyer, L.
AU - Serek, M.
AU - Winkelmann, T.
N1 - Funding Information: Acknowledgments This project was funded by the Federal Ministry of Economy and Technology (BMWi). The authors thank Dr. Andrea Dohm, breeding director Selecta Klemm GmbH, for excellent cooperation during this study, and Professor Errol Hewett (Massey University, Palmerston North, New Zealand) for linguistic editing of the manuscript.
PY - 2009/8/8
Y1 - 2009/8/8
N2 - Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.
AB - Protoplasts were isolated from leaves of in vitro-grown shoot cultures of different Petunia and Calibrachoa genotypes by enzyme digestion with 0.6% macerozyme R-10 and 2.0% cellulase R-10. Shoot regeneration was achieved in five out of nine Calibrachoa and three out of four Petunia genotypes. Protoplast yield and frequency of shoot regeneration varied among experiments and genotypes. Among all regenerants, few morphological changes, such as chlorophyll deficiency, variegated leaves, and polyploidization, were observed.
KW - Callus formation
KW - Plant regeneration and stability
KW - Protoplast yield
UR - http://www.scopus.com/inward/record.url?scp=70350196392&partnerID=8YFLogxK
U2 - 10.1007/s11240-009-9572-4
DO - 10.1007/s11240-009-9572-4
M3 - Article
AN - SCOPUS:70350196392
VL - 99
SP - 27
EP - 34
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -