Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding |
| Publisher | International Society for Horticultural Science |
| Pages | 187-190 |
| Number of pages | 4 |
| ISBN (print) | 906605719X, 9789066057197 |
| Publication status | Published - Nov 2006 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 725 I |
| ISSN (Print) | 0567-7572 |
Abstract
Protoplasts were isolated from six different Petunia hybrida genotypes and cultured embedded in alginate in liquid medium 8pm7 (1.0 mg L-1 benzylaminopurin (BAP) + 1.0 mg L-1 indole acetic acid (IAA) + 2.5% sucrose). This medium was replaced after seven to nine days by callus induction medium KF1 (0.5 mg L-1 BAP + 0.5 mg L-1 IAA and 1.8% sucrose) and later by KF2 (0.5 mg L-1 BAP + 0.5 mg L-1 IAA and 1.3% sucrose). Thereafter, calluses were transferred to solidified PET 2 medium (1 mg L-1 BAP + 2 mg L-1 IAA). The protoplast yield varied between 0.5 and 5.6 * 106 protoplasts per gram fresh weight. Cell division frequencies between 15 and 55% were observed. Shoot regeneration was obtained from three genotypes. In terms of a later fusion and selection of the heterologous hybrids the effects of the cell cycle inhibitors Rhodamine 6G (R6G) and Iodoacetamide (IOA) were analysed. The treatment with different concentrations of R6G and IOA reduced cell division markedly, but pronounced differences between experiments did not allow the identification of a appropriate concentration yet.
Keywords
- Iodoacetamide, Isolation, Plating efficiency, Regeneration, Rhodamine 6G
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding. International Society for Horticultural Science, 2006. p. 187-190 (Acta Horticulturae; Vol. 725 I).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Protoplast culture in Petunia hybrida and effects of cell cycle inhibitors on cell division frequency
AU - Meyer, L.
AU - Serek, M.
AU - Winkelmann, T.
PY - 2006/11
Y1 - 2006/11
N2 - Protoplasts were isolated from six different Petunia hybrida genotypes and cultured embedded in alginate in liquid medium 8pm7 (1.0 mg L-1 benzylaminopurin (BAP) + 1.0 mg L-1 indole acetic acid (IAA) + 2.5% sucrose). This medium was replaced after seven to nine days by callus induction medium KF1 (0.5 mg L-1 BAP + 0.5 mg L-1 IAA and 1.8% sucrose) and later by KF2 (0.5 mg L-1 BAP + 0.5 mg L-1 IAA and 1.3% sucrose). Thereafter, calluses were transferred to solidified PET 2 medium (1 mg L-1 BAP + 2 mg L-1 IAA). The protoplast yield varied between 0.5 and 5.6 * 106 protoplasts per gram fresh weight. Cell division frequencies between 15 and 55% were observed. Shoot regeneration was obtained from three genotypes. In terms of a later fusion and selection of the heterologous hybrids the effects of the cell cycle inhibitors Rhodamine 6G (R6G) and Iodoacetamide (IOA) were analysed. The treatment with different concentrations of R6G and IOA reduced cell division markedly, but pronounced differences between experiments did not allow the identification of a appropriate concentration yet.
AB - Protoplasts were isolated from six different Petunia hybrida genotypes and cultured embedded in alginate in liquid medium 8pm7 (1.0 mg L-1 benzylaminopurin (BAP) + 1.0 mg L-1 indole acetic acid (IAA) + 2.5% sucrose). This medium was replaced after seven to nine days by callus induction medium KF1 (0.5 mg L-1 BAP + 0.5 mg L-1 IAA and 1.8% sucrose) and later by KF2 (0.5 mg L-1 BAP + 0.5 mg L-1 IAA and 1.3% sucrose). Thereafter, calluses were transferred to solidified PET 2 medium (1 mg L-1 BAP + 2 mg L-1 IAA). The protoplast yield varied between 0.5 and 5.6 * 106 protoplasts per gram fresh weight. Cell division frequencies between 15 and 55% were observed. Shoot regeneration was obtained from three genotypes. In terms of a later fusion and selection of the heterologous hybrids the effects of the cell cycle inhibitors Rhodamine 6G (R6G) and Iodoacetamide (IOA) were analysed. The treatment with different concentrations of R6G and IOA reduced cell division markedly, but pronounced differences between experiments did not allow the identification of a appropriate concentration yet.
KW - Iodoacetamide
KW - Isolation
KW - Plating efficiency
KW - Regeneration
KW - Rhodamine 6G
UR - http://www.scopus.com/inward/record.url?scp=33846511130&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2006.725.21
DO - 10.17660/ActaHortic.2006.725.21
M3 - Conference contribution
AN - SCOPUS:33846511130
SN - 906605719X
SN - 9789066057197
T3 - Acta Horticulturae
SP - 187
EP - 190
BT - Proceedings of the Fifth International Symposium on In Vitro Culture and Horticultural Breeding
PB - International Society for Horticultural Science
ER -