Details
| Original language | English |
|---|---|
| Pages (from-to) | 5667-5694 |
| Number of pages | 28 |
| Journal | Journal of proteomics |
| Volume | 75 |
| Issue number | 18 |
| Publication status | Published - 29 Aug 2012 |
Abstract
Responses of the halophyte Cakile maritima to moderate salinity were addressed at germination and vegetative stages by bringing together proteomics and eco-physiological approaches. 75. mM NaCl-salinity delayed significantly the germination process and decreased slightly the seed germination percentage compared to salt-free conditions. Monitoring the proteome profile between 0. h and 120. h after seed sowing revealed a delay in the degradation of seed storage proteins when germination took place under salinity, which may explain the slower germination rate observed. Of the sixty-seven proteins identified by mass spectrometry, several proteins involved in glycolysis, amino acid metabolism, photosynthesis, and protein folding showed significantly increased abundance during germination. This pattern was less pronounced under salinity. At the vegetative stage, 100. mM NaCl-salinity stimulated significantly the plant growth, which was sustained by enhanced leaf expansion, water content, and photosynthetic activity. Comparative proteome analyses of leaf tissue revealed 44 proteins with different abundance changes, most of which being involved in energy metabolism. A specific set of proteins predominantly involved in photosynthesis and respiration showed significantly higher abundance in salt-treated plants. Altogether, combining proteomics with eco-physiological tools provides valuable information, which contributes to improve our understanding in the salt-response of this halophyte during its life cycle.
Keywords
- Germination, Halophytes, Leaves, Proteome, Salt-tolerance
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Sustainable Development Goals
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In: Journal of proteomics, Vol. 75, No. 18, 29.08.2012, p. 5667-5694.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Proteomic and physiological responses of the halophyte Cakile maritima to moderate salinity at the germinative and vegetative stages
AU - Debez, Ahmed
AU - Braun, Hans Peter
AU - Pich, Andreas
AU - Taamalli, Wael
AU - Koyro, Hans Werner
AU - Abdelly, Chedly
AU - Huchzermeyer, Bernhard
N1 - Funding information: This work was financially supported by the Alexander von Humboldt Foundation . The excellent technical assistance of Dagmar Lewejohann and the help of Malte Regelin with respect to the proteomic experiments and the 2D-PAGE gel analysis respectively are gratefully acknowledged. The critical reading of Dr. Wahbi Djebali was highly appreciated.
PY - 2012/8/29
Y1 - 2012/8/29
N2 - Responses of the halophyte Cakile maritima to moderate salinity were addressed at germination and vegetative stages by bringing together proteomics and eco-physiological approaches. 75. mM NaCl-salinity delayed significantly the germination process and decreased slightly the seed germination percentage compared to salt-free conditions. Monitoring the proteome profile between 0. h and 120. h after seed sowing revealed a delay in the degradation of seed storage proteins when germination took place under salinity, which may explain the slower germination rate observed. Of the sixty-seven proteins identified by mass spectrometry, several proteins involved in glycolysis, amino acid metabolism, photosynthesis, and protein folding showed significantly increased abundance during germination. This pattern was less pronounced under salinity. At the vegetative stage, 100. mM NaCl-salinity stimulated significantly the plant growth, which was sustained by enhanced leaf expansion, water content, and photosynthetic activity. Comparative proteome analyses of leaf tissue revealed 44 proteins with different abundance changes, most of which being involved in energy metabolism. A specific set of proteins predominantly involved in photosynthesis and respiration showed significantly higher abundance in salt-treated plants. Altogether, combining proteomics with eco-physiological tools provides valuable information, which contributes to improve our understanding in the salt-response of this halophyte during its life cycle.
AB - Responses of the halophyte Cakile maritima to moderate salinity were addressed at germination and vegetative stages by bringing together proteomics and eco-physiological approaches. 75. mM NaCl-salinity delayed significantly the germination process and decreased slightly the seed germination percentage compared to salt-free conditions. Monitoring the proteome profile between 0. h and 120. h after seed sowing revealed a delay in the degradation of seed storage proteins when germination took place under salinity, which may explain the slower germination rate observed. Of the sixty-seven proteins identified by mass spectrometry, several proteins involved in glycolysis, amino acid metabolism, photosynthesis, and protein folding showed significantly increased abundance during germination. This pattern was less pronounced under salinity. At the vegetative stage, 100. mM NaCl-salinity stimulated significantly the plant growth, which was sustained by enhanced leaf expansion, water content, and photosynthetic activity. Comparative proteome analyses of leaf tissue revealed 44 proteins with different abundance changes, most of which being involved in energy metabolism. A specific set of proteins predominantly involved in photosynthesis and respiration showed significantly higher abundance in salt-treated plants. Altogether, combining proteomics with eco-physiological tools provides valuable information, which contributes to improve our understanding in the salt-response of this halophyte during its life cycle.
KW - Germination
KW - Halophytes
KW - Leaves
KW - Proteome
KW - Salt-tolerance
UR - http://www.scopus.com/inward/record.url?scp=84866184257&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2012.08.012
DO - 10.1016/j.jprot.2012.08.012
M3 - Article
C2 - 22940175
AN - SCOPUS:84866184257
VL - 75
SP - 5667
EP - 5694
JO - Journal of proteomics
JF - Journal of proteomics
SN - 1874-3919
IS - 18
ER -