Details
| Original language | English |
|---|---|
| Pages (from-to) | 52-65 |
| Number of pages | 14 |
| Journal | Plant science |
| Volume | 201-202 |
| Issue number | 1 |
| Publication status | Published - 27 Nov 2012 |
Abstract
The endosperm plays an important role for the development of zygotic embryos, while somatic embryos lack a seed coat and endosperm and often show physiological disorders. This study aims at elucidating the cellular and physiological processes within the endosperm of the ornamental species Cyclamen persicum Mill. Histological analyses were performed from 0 to 11 weeks after pollination (WAP). At 3. WAP, a syncytium was clearly visible with a globular zygotic embryo. From 4. WAP, cellularization of the endosperm, at 5. WAP a small torpedo shaped embryo, and from 7. WAP cell expansion was observed. By 11. WAP the endosperm appeared fully differentiated. Total soluble proteins were extracted from the endosperm at 4, 5, 7, 9 and 11. WAP and resolved using two dimensional isoelectric focussing/sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D IEF/SDS-PAGE). A shift from high-molecular-mass proteins to low-molecular-mass proteins during endosperm development was observed. A total of 1137. protein. spots/gel were detected in the three protein fractions extracted at 7, 9 and 11. WAP. Mass spectrometry analysis of the 48 predominant protein spots in endosperm at 7, 9 and 11. WAP resulted in the identification of 62 proteins, ten of which were described for the first time in Cyclamen. Additionally, 186 proteins were identified using the C. persicum embryo proteome reference map. Proteins involved in abscisic acid signalling and oxidative stress responsive proteins were found to be important for seed development in Cyclamen. The new insights into endosperm physiology including storage compounds are discussed.
Keywords
- Cyclamen persicum, Endosperm, Mass spectrometry, Proteomics, Seed development
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant science, Vol. 201-202, No. 1, 27.11.2012, p. 52-65.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Proteomic and histological analyses of endosperm development in Cyclamen persicum as a basis for optimization of somatic embryogenesis
AU - Mwangi, Jenniffer Wamaitha
AU - Rode, Christina
AU - Colditz, Frank
AU - Haase, Christin
AU - Braun, Hans Peter
AU - Winkelmann, Traud
N1 - Funding information: The authors thank the DAAD (German Academic Exchange Service) for financial support of Jenniffer Mwangi, Kathrin Lindhorst for excellent technical assistance, Dr. Annette Hohe and Katja Krüger of Leibniz-Institute of Vegetable and Ornamental Crops in Erfurt for support with respect to histological analyses.
PY - 2012/11/27
Y1 - 2012/11/27
N2 - The endosperm plays an important role for the development of zygotic embryos, while somatic embryos lack a seed coat and endosperm and often show physiological disorders. This study aims at elucidating the cellular and physiological processes within the endosperm of the ornamental species Cyclamen persicum Mill. Histological analyses were performed from 0 to 11 weeks after pollination (WAP). At 3. WAP, a syncytium was clearly visible with a globular zygotic embryo. From 4. WAP, cellularization of the endosperm, at 5. WAP a small torpedo shaped embryo, and from 7. WAP cell expansion was observed. By 11. WAP the endosperm appeared fully differentiated. Total soluble proteins were extracted from the endosperm at 4, 5, 7, 9 and 11. WAP and resolved using two dimensional isoelectric focussing/sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D IEF/SDS-PAGE). A shift from high-molecular-mass proteins to low-molecular-mass proteins during endosperm development was observed. A total of 1137. protein. spots/gel were detected in the three protein fractions extracted at 7, 9 and 11. WAP. Mass spectrometry analysis of the 48 predominant protein spots in endosperm at 7, 9 and 11. WAP resulted in the identification of 62 proteins, ten of which were described for the first time in Cyclamen. Additionally, 186 proteins were identified using the C. persicum embryo proteome reference map. Proteins involved in abscisic acid signalling and oxidative stress responsive proteins were found to be important for seed development in Cyclamen. The new insights into endosperm physiology including storage compounds are discussed.
AB - The endosperm plays an important role for the development of zygotic embryos, while somatic embryos lack a seed coat and endosperm and often show physiological disorders. This study aims at elucidating the cellular and physiological processes within the endosperm of the ornamental species Cyclamen persicum Mill. Histological analyses were performed from 0 to 11 weeks after pollination (WAP). At 3. WAP, a syncytium was clearly visible with a globular zygotic embryo. From 4. WAP, cellularization of the endosperm, at 5. WAP a small torpedo shaped embryo, and from 7. WAP cell expansion was observed. By 11. WAP the endosperm appeared fully differentiated. Total soluble proteins were extracted from the endosperm at 4, 5, 7, 9 and 11. WAP and resolved using two dimensional isoelectric focussing/sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D IEF/SDS-PAGE). A shift from high-molecular-mass proteins to low-molecular-mass proteins during endosperm development was observed. A total of 1137. protein. spots/gel were detected in the three protein fractions extracted at 7, 9 and 11. WAP. Mass spectrometry analysis of the 48 predominant protein spots in endosperm at 7, 9 and 11. WAP resulted in the identification of 62 proteins, ten of which were described for the first time in Cyclamen. Additionally, 186 proteins were identified using the C. persicum embryo proteome reference map. Proteins involved in abscisic acid signalling and oxidative stress responsive proteins were found to be important for seed development in Cyclamen. The new insights into endosperm physiology including storage compounds are discussed.
KW - Cyclamen persicum
KW - Endosperm
KW - Mass spectrometry
KW - Proteomics
KW - Seed development
UR - http://www.scopus.com/inward/record.url?scp=84871538853&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2012.11.004
DO - 10.1016/j.plantsci.2012.11.004
M3 - Article
C2 - 23352402
AN - SCOPUS:84871538853
VL - 201-202
SP - 52
EP - 65
JO - Plant science
JF - Plant science
SN - 0168-9452
IS - 1
ER -