Details
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 22nd International Eucarpia Fymposium Section Ornamentals |
| Editors | A. Mercuri, T. Schiva |
| Pages | 163-169 |
| Number of pages | 7 |
| Publication status | Published - Sept 2006 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 714 |
| ISSN (Print) | 0567-7572 |
Abstract
In the horticulturally important ornamental species Cyclamen persicum somatic embryogenesis is an efficient vegetative propagation method and interest is given in the development of artificial seeds. Previous studies have shown that cyclamen somatic embryos can successfully be encapsulated and desiccated, but need external supply of nutrients for germination. Objectives of the present study were to systematically compare the proteomes of zygotic embryos, somatic embryos grown in liquid medium containing 30 or 60 g L-1 sucrose, germinating embryos of both types and endosperm in order to obtain novel insights into seed and germination physiology. Using high resolution two-dimensional isoelectric focusing/sodium dodecylsulfate polyacrylamide gel electrophoresis (2D IEF/SDS PAGE), 74% of the proteins expressed in zygotic embryos were found in similar abundance in somatic embryos grown in 60 g L -1 sucrose. Among the differentially accumulating proteins, four enzymes of the primary metabolism involved in glycolysis (UDP-glucose pyrophosphorylase, fructose bisphosphate aldolase, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase) were specifically induced in somatic embryos. Proteins of high abundance in embryos and endosperm and comparatively low abundance in germinating embryos represent candidates for seed storage proteins, which so far were unknown for C. persicum. 11S globulin proteins identified by mass spectrometry were present at high levels in somatic embryos, zygotic embryos and endosperm, whereas 7S globulins were detected in endosperm and zygotic embryos mainly. Xyloglucans are known to be another group of seed storage compounds in C. persicum. Their formation was not studied so far. Interestingly, xyloglucan endotransglycosylases, an enzyme group involved in xyloglucan biosynthesis but also its degradation were found to be highly expressed in endosperm tissue.
Keywords
- Artificial seeds, Mass spectrometry, Ornamental plant, Somatic embryogenesis, Storage proteins, Two-dimensional polyacrylamide gel electrophoresis, Xyloglucan endotransglycosylase
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Proceedings of the 22nd International Eucarpia Fymposium Section Ornamentals. ed. / A. Mercuri; T. Schiva. 2006. p. 163-169 (Acta Horticulturae; Vol. 714).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Proteomic Analyses of Somatic and Zygotic Embryos and Endosperm Tissue of Cyclamen persicum
AU - Winkelmann, T.
AU - Heintz, D.
AU - Van Dorsselaer, A.
AU - Serek, M.
AU - Braun, H. P.
PY - 2006/9
Y1 - 2006/9
N2 - In the horticulturally important ornamental species Cyclamen persicum somatic embryogenesis is an efficient vegetative propagation method and interest is given in the development of artificial seeds. Previous studies have shown that cyclamen somatic embryos can successfully be encapsulated and desiccated, but need external supply of nutrients for germination. Objectives of the present study were to systematically compare the proteomes of zygotic embryos, somatic embryos grown in liquid medium containing 30 or 60 g L-1 sucrose, germinating embryos of both types and endosperm in order to obtain novel insights into seed and germination physiology. Using high resolution two-dimensional isoelectric focusing/sodium dodecylsulfate polyacrylamide gel electrophoresis (2D IEF/SDS PAGE), 74% of the proteins expressed in zygotic embryos were found in similar abundance in somatic embryos grown in 60 g L -1 sucrose. Among the differentially accumulating proteins, four enzymes of the primary metabolism involved in glycolysis (UDP-glucose pyrophosphorylase, fructose bisphosphate aldolase, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase) were specifically induced in somatic embryos. Proteins of high abundance in embryos and endosperm and comparatively low abundance in germinating embryos represent candidates for seed storage proteins, which so far were unknown for C. persicum. 11S globulin proteins identified by mass spectrometry were present at high levels in somatic embryos, zygotic embryos and endosperm, whereas 7S globulins were detected in endosperm and zygotic embryos mainly. Xyloglucans are known to be another group of seed storage compounds in C. persicum. Their formation was not studied so far. Interestingly, xyloglucan endotransglycosylases, an enzyme group involved in xyloglucan biosynthesis but also its degradation were found to be highly expressed in endosperm tissue.
AB - In the horticulturally important ornamental species Cyclamen persicum somatic embryogenesis is an efficient vegetative propagation method and interest is given in the development of artificial seeds. Previous studies have shown that cyclamen somatic embryos can successfully be encapsulated and desiccated, but need external supply of nutrients for germination. Objectives of the present study were to systematically compare the proteomes of zygotic embryos, somatic embryos grown in liquid medium containing 30 or 60 g L-1 sucrose, germinating embryos of both types and endosperm in order to obtain novel insights into seed and germination physiology. Using high resolution two-dimensional isoelectric focusing/sodium dodecylsulfate polyacrylamide gel electrophoresis (2D IEF/SDS PAGE), 74% of the proteins expressed in zygotic embryos were found in similar abundance in somatic embryos grown in 60 g L -1 sucrose. Among the differentially accumulating proteins, four enzymes of the primary metabolism involved in glycolysis (UDP-glucose pyrophosphorylase, fructose bisphosphate aldolase, triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase) were specifically induced in somatic embryos. Proteins of high abundance in embryos and endosperm and comparatively low abundance in germinating embryos represent candidates for seed storage proteins, which so far were unknown for C. persicum. 11S globulin proteins identified by mass spectrometry were present at high levels in somatic embryos, zygotic embryos and endosperm, whereas 7S globulins were detected in endosperm and zygotic embryos mainly. Xyloglucans are known to be another group of seed storage compounds in C. persicum. Their formation was not studied so far. Interestingly, xyloglucan endotransglycosylases, an enzyme group involved in xyloglucan biosynthesis but also its degradation were found to be highly expressed in endosperm tissue.
KW - Artificial seeds
KW - Mass spectrometry
KW - Ornamental plant
KW - Somatic embryogenesis
KW - Storage proteins
KW - Two-dimensional polyacrylamide gel electrophoresis
KW - Xyloglucan endotransglycosylase
UR - http://www.scopus.com/inward/record.url?scp=33750293038&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2006.714.18
DO - 10.17660/ActaHortic.2006.714.18
M3 - Conference contribution
AN - SCOPUS:33750293038
SN - 9066055499
SN - 9789066055490
T3 - Acta Horticulturae
SP - 163
EP - 169
BT - Proceedings of the 22nd International Eucarpia Fymposium Section Ornamentals
A2 - Mercuri, A.
A2 - Schiva, T.
ER -