Details
| Original language | English |
|---|---|
| Pages (from-to) | 4891-4903 |
| Number of pages | 13 |
| Journal | Journal of experimental botany |
| Volume | 75 |
| Issue number | 16 |
| Early online date | 30 Apr 2024 |
| Publication status | Published - 28 Aug 2024 |
Abstract
During germination plants rely entirely on their seed storage compounds to provide energy and precursors for the synthesis of macromolecular structures until the seedling has emerged from the soil and photosynthesis can be established. Lupin seeds use proteins as their major storage compounds, accounting for up to 40% of the seed dry weight. Lupins are therefore a valuable complement to soy as a source of plant protein for human and animal nutrition. The aim of this study was to elucidate how storage protein metabolism is coordinated with other metabolic processes to meet the requirements of the growing seedling. In a quantitative approach, we analyzed seedling growth, as well as alterations in biomass composition, the proteome, and metabolite profiles during germination and seedling establishment in Lupinus albus. The reallocation of nitrogen resources from seed storage proteins to functional seed proteins was mapped based on a manually curated functional protein annotation database. Although classified as a protein crop, Lupinus albus does not use amino acids as a primary substrate for energy metabolism during germination. However, fatty acid and amino acid metabolism may be integrated at the level of malate synthase to combine stored carbon from lipids and proteins into gluconeogenesis.
Keywords
- Amino acid metabolism, Lupinus albus protein annotation database, germination, glyoxylate cycle, nitrogen resource allocation, proteomics
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of experimental botany, Vol. 75, No. 16, 28.08.2024, p. 4891-4903.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Proteome reorganization and amino acid metabolism during germination and seedling establishment in Lupinus albus
AU - Angermann, Cecile
AU - Heinemann, Björn
AU - Hansen, Jule
AU - Töpfer, Nadine
AU - Braun, Hans-Peter
AU - Hildebrandt, Tatjana M
N1 - Publisher Copyright: © 2024 The Author(s). Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved.
PY - 2024/8/28
Y1 - 2024/8/28
N2 - During germination plants rely entirely on their seed storage compounds to provide energy and precursors for the synthesis of macromolecular structures until the seedling has emerged from the soil and photosynthesis can be established. Lupin seeds use proteins as their major storage compounds, accounting for up to 40% of the seed dry weight. Lupins are therefore a valuable complement to soy as a source of plant protein for human and animal nutrition. The aim of this study was to elucidate how storage protein metabolism is coordinated with other metabolic processes to meet the requirements of the growing seedling. In a quantitative approach, we analyzed seedling growth, as well as alterations in biomass composition, the proteome, and metabolite profiles during germination and seedling establishment in Lupinus albus. The reallocation of nitrogen resources from seed storage proteins to functional seed proteins was mapped based on a manually curated functional protein annotation database. Although classified as a protein crop, Lupinus albus does not use amino acids as a primary substrate for energy metabolism during germination. However, fatty acid and amino acid metabolism may be integrated at the level of malate synthase to combine stored carbon from lipids and proteins into gluconeogenesis.
AB - During germination plants rely entirely on their seed storage compounds to provide energy and precursors for the synthesis of macromolecular structures until the seedling has emerged from the soil and photosynthesis can be established. Lupin seeds use proteins as their major storage compounds, accounting for up to 40% of the seed dry weight. Lupins are therefore a valuable complement to soy as a source of plant protein for human and animal nutrition. The aim of this study was to elucidate how storage protein metabolism is coordinated with other metabolic processes to meet the requirements of the growing seedling. In a quantitative approach, we analyzed seedling growth, as well as alterations in biomass composition, the proteome, and metabolite profiles during germination and seedling establishment in Lupinus albus. The reallocation of nitrogen resources from seed storage proteins to functional seed proteins was mapped based on a manually curated functional protein annotation database. Although classified as a protein crop, Lupinus albus does not use amino acids as a primary substrate for energy metabolism during germination. However, fatty acid and amino acid metabolism may be integrated at the level of malate synthase to combine stored carbon from lipids and proteins into gluconeogenesis.
KW - Amino acid metabolism
KW - Lupinus albus protein annotation database
KW - germination
KW - glyoxylate cycle
KW - nitrogen resource allocation
KW - proteomics
UR - http://www.scopus.com/inward/record.url?scp=85202671519&partnerID=8YFLogxK
U2 - 10.1093/jxb/erae197
DO - 10.1093/jxb/erae197
M3 - Article
C2 - 38686677
VL - 75
SP - 4891
EP - 4903
JO - Journal of experimental botany
JF - Journal of experimental botany
SN - 0022-0957
IS - 16
ER -