Details
| Original language | English |
|---|---|
| Pages (from-to) | 103-108 |
| Number of pages | 6 |
| Journal | Engineering in life sciences |
| Volume | 10 |
| Issue number | 2 |
| Publication status | Published - 12 Apr 2010 |
Abstract
In protein microarray performance, the choice of an appropriate surface is a crucial factor. Three-dimensional substrates like nitrocellulose are known to have higher binding capacities than planar surfaces. Furthermore, they can enable the immobilization of proteins in a functional manner. One disadvantage of today's nitrocellulose-based microarrays is the high background fluorescence, which can interfere with the detection of low-abundance proteins. We have developed an innovative black nitrocellulose membrane-based protein microarray that exhibits low autofluorescence in combination with increased sensitivity and improved LOD (limit of detection). The applicability of the novel material was demonstrated with main focus on reversed-phase microarray experiments. In comparison to various commercially available microarrays, a higher sensitivity in regard to the spotted protein was achieved. In contrast to other porous nitrocellulose-based microarrays, the black nitrocellulose provides a significant lower autofluorescence and background intensity.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Environmental Science(all)
- Environmental Engineering
- Chemical Engineering(all)
- Bioengineering
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In: Engineering in life sciences, Vol. 10, No. 2, 12.04.2010, p. 103-108.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Protein microarrays: Reducedautofluorescence and improved LOD
AU - Walter, Johanna Gabriela
AU - Stahl, Frank
AU - Reck, Michael
AU - Praulich, Inka
AU - Nataf, Yakir
AU - Hollas, Markus
AU - Pflanz, Karl
AU - Melzner, Dieter
AU - Shoham, Yuval
AU - Scheper, Thomas
PY - 2010/4/12
Y1 - 2010/4/12
N2 - In protein microarray performance, the choice of an appropriate surface is a crucial factor. Three-dimensional substrates like nitrocellulose are known to have higher binding capacities than planar surfaces. Furthermore, they can enable the immobilization of proteins in a functional manner. One disadvantage of today's nitrocellulose-based microarrays is the high background fluorescence, which can interfere with the detection of low-abundance proteins. We have developed an innovative black nitrocellulose membrane-based protein microarray that exhibits low autofluorescence in combination with increased sensitivity and improved LOD (limit of detection). The applicability of the novel material was demonstrated with main focus on reversed-phase microarray experiments. In comparison to various commercially available microarrays, a higher sensitivity in regard to the spotted protein was achieved. In contrast to other porous nitrocellulose-based microarrays, the black nitrocellulose provides a significant lower autofluorescence and background intensity.
AB - In protein microarray performance, the choice of an appropriate surface is a crucial factor. Three-dimensional substrates like nitrocellulose are known to have higher binding capacities than planar surfaces. Furthermore, they can enable the immobilization of proteins in a functional manner. One disadvantage of today's nitrocellulose-based microarrays is the high background fluorescence, which can interfere with the detection of low-abundance proteins. We have developed an innovative black nitrocellulose membrane-based protein microarray that exhibits low autofluorescence in combination with increased sensitivity and improved LOD (limit of detection). The applicability of the novel material was demonstrated with main focus on reversed-phase microarray experiments. In comparison to various commercially available microarrays, a higher sensitivity in regard to the spotted protein was achieved. In contrast to other porous nitrocellulose-based microarrays, the black nitrocellulose provides a significant lower autofluorescence and background intensity.
UR - http://www.scopus.com/inward/record.url?scp=77950790350&partnerID=8YFLogxK
U2 - 10.1002/elsc.200900078
DO - 10.1002/elsc.200900078
M3 - Article
AN - SCOPUS:77950790350
VL - 10
SP - 103
EP - 108
JO - Engineering in life sciences
JF - Engineering in life sciences
SN - 1618-0240
IS - 2
ER -