Details
| Original language | English |
|---|---|
| Article number | 11 |
| Journal | Microbial cell factories |
| Volume | 7 |
| Publication status | Published - 4 Apr 2008 |
| Externally published | Yes |
Abstract
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Microbial cell factories, Vol. 7, 11, 04.04.2008.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Protein folding and conformational stress in microbial cells producing recombinant proteins
T2 - A host comparative overview
AU - Gasser, Brigitte
AU - Saloheimo, Markku
AU - Rinas, Ursula
AU - Dragosits, Martin
AU - Rodríguez-Carmona, Escarlata
AU - Baumann, Kristin
AU - Giuliani, Maria
AU - Parrilli, Ermenegilda
AU - Branduardi, Paola
AU - Lang, Christine
AU - Porro, Danilo
AU - Ferrer, Pau
AU - Tutino, Maria
AU - Mattanovich, Diethard
AU - Villaverde, Antonio
N1 - Funding Information: The authors gratefully acknowledge the financial support provided by MEC, Spain and Austria Science Fund through the EUROCORES Programmes EuroSCOPE of the European Science Foundation, which is also funded by the European Commission, Contract no. ERAS-CT-2003-980409 of the Sixth Framework Programme. References of received grants are: to PF: Complementary Actions Plan from the Spanish MEC, Project No. BIO2005-23733-E. AGAUR (Generalitat de Catalunya, Spain), Grant 2005SGR 00698 and Reference Network in Biotechnology (XerBa).
PY - 2008/4/4
Y1 - 2008/4/4
N2 - Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
AB - Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.
UR - http://www.scopus.com/inward/record.url?scp=42549105668&partnerID=8YFLogxK
U2 - 10.1186/1475-2859-7-11
DO - 10.1186/1475-2859-7-11
M3 - Review article
AN - SCOPUS:42549105668
VL - 7
JO - Microbial cell factories
JF - Microbial cell factories
SN - 1475-2859
M1 - 11
ER -