Details
| Original language | English |
|---|---|
| Pages (from-to) | 8547-8553 |
| Number of pages | 7 |
| Journal | LANGMUIR |
| Volume | 24 |
| Issue number | 16 |
| Early online date | 17 Jul 2008 |
| Publication status | Published - 1 Aug 2008 |
Abstract
We present a detailed study of the diffusive transport of proteins across a fluid phase boundary within aqueous two-phase systems. The aim of the work is to investigate whether local effects at the phase boundary cause a retardation of the diffusive transport between the phases. Possible modifications of interfacial mass transfer could be due to protein adsorption at the phase boundary or local electric fields from electric double layers. Experiments with a microfluidic system have been performed in which protein diffusion (bovine serum albumin and ovalbumin) within a bilaminated configuration of two phases containing polyethylene glycol and dextran is analyzed. A one-dimensional model incorporating phase-specific diffusion constants and the difference in chemical potential between the phases has been formulated. A comparison of experimental and simulation data shows a good overall agreement and suggests that a potential local influence of the phase boundary on protein transport is insignificant for the systems under investigation.
ASJC Scopus subject areas
- Materials Science(all)
- General Materials Science
- Physics and Astronomy(all)
- Condensed Matter Physics
- Physics and Astronomy(all)
- Surfaces and Interfaces
- Chemistry(all)
- Spectroscopy
- Chemistry(all)
- Electrochemistry
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In: LANGMUIR, Vol. 24, No. 16, 01.08.2008, p. 8547-8553.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Protein Diffusion Across the Interface in Aqueous Two-Phase Systems
AU - Münchow, Götz
AU - Schönfeld, Friedhelm
AU - Hardt, Steffen
AU - Graf, Karlheinz
PY - 2008/8/1
Y1 - 2008/8/1
N2 - We present a detailed study of the diffusive transport of proteins across a fluid phase boundary within aqueous two-phase systems. The aim of the work is to investigate whether local effects at the phase boundary cause a retardation of the diffusive transport between the phases. Possible modifications of interfacial mass transfer could be due to protein adsorption at the phase boundary or local electric fields from electric double layers. Experiments with a microfluidic system have been performed in which protein diffusion (bovine serum albumin and ovalbumin) within a bilaminated configuration of two phases containing polyethylene glycol and dextran is analyzed. A one-dimensional model incorporating phase-specific diffusion constants and the difference in chemical potential between the phases has been formulated. A comparison of experimental and simulation data shows a good overall agreement and suggests that a potential local influence of the phase boundary on protein transport is insignificant for the systems under investigation.
AB - We present a detailed study of the diffusive transport of proteins across a fluid phase boundary within aqueous two-phase systems. The aim of the work is to investigate whether local effects at the phase boundary cause a retardation of the diffusive transport between the phases. Possible modifications of interfacial mass transfer could be due to protein adsorption at the phase boundary or local electric fields from electric double layers. Experiments with a microfluidic system have been performed in which protein diffusion (bovine serum albumin and ovalbumin) within a bilaminated configuration of two phases containing polyethylene glycol and dextran is analyzed. A one-dimensional model incorporating phase-specific diffusion constants and the difference in chemical potential between the phases has been formulated. A comparison of experimental and simulation data shows a good overall agreement and suggests that a potential local influence of the phase boundary on protein transport is insignificant for the systems under investigation.
UR - http://www.scopus.com/inward/record.url?scp=50849121675&partnerID=8YFLogxK
U2 - 10.1021/la800956j
DO - 10.1021/la800956j
M3 - Article
C2 - 18630980
AN - SCOPUS:50849121675
VL - 24
SP - 8547
EP - 8553
JO - LANGMUIR
JF - LANGMUIR
SN - 0743-7463
IS - 16
ER -