Details
| Original language | English |
|---|---|
| Pages (from-to) | 1048-1057 |
| Number of pages | 10 |
| Journal | New Phytologist |
| Volume | 187 |
| Issue number | 4 |
| Publication status | Published - 12 Aug 2010 |
| Externally published | Yes |
Abstract
•Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.•Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via β-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).•We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.•Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.
Keywords
- AvrBs3, AvrXa27, Bs3, PthXo1, UPA (up-regulated by AvrBs3), Xa13, Xanthomonas
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: New Phytologist, Vol. 187, No. 4, 12.08.2010, p. 1048-1057.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Promoter elements of rice susceptibility genes are bound and activated by specific TAL effectors from the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae
AU - Römer, Patrick
AU - Recht, Sabine
AU - Strauß, Tina
AU - Elsaesser, Janett
AU - Schornack, Sebastian
AU - Boch, Jens
AU - Wang, Shiping
AU - Lahaye, Thomas
N1 - Copyright: Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/8/12
Y1 - 2010/8/12
N2 - •Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.•Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via β-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).•We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.•Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.
AB - •Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes.•Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via β-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA).•We demonstrate that the Xa13, OsTFX1 and Os11N3 promoters from rice are induced directly by the Xanthomonas oryzae pv. oryzae TALes PthXo1, PthXo6 and AvrXa7, respectively. We identified and functionally validated a UPT box in the corresponding rice target promoter for each TALe and show that box mutations suppress TALe-mediated promoter activation. Finally, EMSA demonstrate that code-predicted UPT boxes interact specifically with corresponding TALes.•Our findings show that variations in the UPT boxes of different rice accessions correlate with susceptibility or resistance of these accessions to the bacterial blight pathogen.
KW - AvrBs3
KW - AvrXa27
KW - Bs3
KW - PthXo1
KW - UPA (up-regulated by AvrBs3)
KW - Xa13
KW - Xanthomonas
UR - http://www.scopus.com/inward/record.url?scp=77955737033&partnerID=8YFLogxK
U2 - 10.1111/j.1469-8137.2010.03217.x
DO - 10.1111/j.1469-8137.2010.03217.x
M3 - Article
C2 - 20345643
AN - SCOPUS:77955737033
VL - 187
SP - 1048
EP - 1057
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 4
ER -