Details
| Original language | English |
|---|---|
| Article number | 102835 |
| Journal | Current opinion in biotechnology |
| Volume | 78 |
| Publication status | Published - Dec 2022 |
Abstract
In this review, we focus on the holistic continuous enzymatic production and put special emphasis on process intensification by up- and downstream processing in continuous flow biocatalysis. After a brief introduction, we provide an overview of current examples of enzyme immobilization as an upstream process for flow biocatalysis. Thereafter, we provide an overview of unit operations as downstream processing strategies, namely continuous (i) liquid–liquid extraction, (ii) adsorptive downstream processing, and (iii) crystallization and precipitation. Eventually, we present our perspectives on future trends in this research field.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Engineering(all)
- Biomedical Engineering
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In: Current opinion in biotechnology, Vol. 78, 102835, 12.2022.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Process intensification in continuous flow biocatalysis by up and downstream processing strategies
AU - Meyer, Lars-Erik
AU - Hobisch, Markus
AU - Kara, Selin
N1 - Funding Information: S.K. gratefully acknowledges the Independent Research Fund Denmark (PHOTOX-f project, grant No 9063-00031B ) for the grant funding in the framework of Sapere Aude DFF-Starting Grant. Aarhus University Research Foundation (AUFF, Aarhus Universitets Forskningsfond) is acknowledged for financial support.
PY - 2022/12
Y1 - 2022/12
N2 - In this review, we focus on the holistic continuous enzymatic production and put special emphasis on process intensification by up- and downstream processing in continuous flow biocatalysis. After a brief introduction, we provide an overview of current examples of enzyme immobilization as an upstream process for flow biocatalysis. Thereafter, we provide an overview of unit operations as downstream processing strategies, namely continuous (i) liquid–liquid extraction, (ii) adsorptive downstream processing, and (iii) crystallization and precipitation. Eventually, we present our perspectives on future trends in this research field.
AB - In this review, we focus on the holistic continuous enzymatic production and put special emphasis on process intensification by up- and downstream processing in continuous flow biocatalysis. After a brief introduction, we provide an overview of current examples of enzyme immobilization as an upstream process for flow biocatalysis. Thereafter, we provide an overview of unit operations as downstream processing strategies, namely continuous (i) liquid–liquid extraction, (ii) adsorptive downstream processing, and (iii) crystallization and precipitation. Eventually, we present our perspectives on future trends in this research field.
UR - http://www.scopus.com/inward/record.url?scp=85140958680&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2022.102835
DO - 10.1016/j.copbio.2022.102835
M3 - Review article
C2 - 36332339
VL - 78
JO - Current opinion in biotechnology
JF - Current opinion in biotechnology
SN - 0958-1669
M1 - 102835
ER -