Details
| Original language | English |
|---|---|
| Article number | 598459 |
| Journal | Frontiers in Bioengineering and Biotechnology |
| Volume | 8 |
| Publication status | Published - 17 Nov 2020 |
Abstract
This work probes the binding kinetics of COOH-terminus of Clostridium perfringens enterotoxin (c-CPE) and claudin expressing MCF-7 cells using force spectroscopy with optical tweezers. c-CPE is of high biomedical interest due to its ability to specifically bind to claudin with high affinity as well as reversibly disrupt tight junctions whilst maintaining cell viability. We observed single-step rupture events between silica particles functionalized with c-CPE and MCF-7 cells. Extensive calibration of the optical tweezers’ trap stiffness and displacement of the particle from trap center extracted a probable bond rupture force of ≈ 18 pN. The probability of rupture events with c-CPE functionalized silica particles increased by 50% compared to unfunctionalized particles. Additionally, rupture events were not observed when probing cells not expressing claudin with c-CPE coated particles. Overall, this work demonstrates that optical tweezers are invaluable tools to probe ligand-receptor interactions and their potential to study dynamic molecular events in drug-binding scenarios.
Keywords
- C-CPE, cell receptors, claudin, force spectroscopy, ligand, optical tweezers
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Histology
- Engineering(all)
- Biomedical Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Frontiers in Bioengineering and Biotechnology, Vol. 8, 598459, 17.11.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Probing Ligand-Receptor Interaction in Living Cells Using Force Measurements With Optical Tweezers
AU - Riesenberg, Carolin
AU - Iriarte-Valdez, Christian Alejandro
AU - Becker, Annegret
AU - Dienerowitz, Maria
AU - Heisterkamp, Alexander
AU - Ngezahayo, Anaclet
AU - Torres, Maria Leilani
N1 - Funding Information: This work was supported by the German Research Foundation, Germany Clusters of Excellence: REBIRTH (EXC 62) and Hearing4all (EXC 2177). MT-M acknowledges the support of Caroline Herschel Program from the Hochschulbüro für Chancenvielfalt, Leibniz University Hannover.
PY - 2020/11/17
Y1 - 2020/11/17
N2 - This work probes the binding kinetics of COOH-terminus of Clostridium perfringens enterotoxin (c-CPE) and claudin expressing MCF-7 cells using force spectroscopy with optical tweezers. c-CPE is of high biomedical interest due to its ability to specifically bind to claudin with high affinity as well as reversibly disrupt tight junctions whilst maintaining cell viability. We observed single-step rupture events between silica particles functionalized with c-CPE and MCF-7 cells. Extensive calibration of the optical tweezers’ trap stiffness and displacement of the particle from trap center extracted a probable bond rupture force of ≈ 18 pN. The probability of rupture events with c-CPE functionalized silica particles increased by 50% compared to unfunctionalized particles. Additionally, rupture events were not observed when probing cells not expressing claudin with c-CPE coated particles. Overall, this work demonstrates that optical tweezers are invaluable tools to probe ligand-receptor interactions and their potential to study dynamic molecular events in drug-binding scenarios.
AB - This work probes the binding kinetics of COOH-terminus of Clostridium perfringens enterotoxin (c-CPE) and claudin expressing MCF-7 cells using force spectroscopy with optical tweezers. c-CPE is of high biomedical interest due to its ability to specifically bind to claudin with high affinity as well as reversibly disrupt tight junctions whilst maintaining cell viability. We observed single-step rupture events between silica particles functionalized with c-CPE and MCF-7 cells. Extensive calibration of the optical tweezers’ trap stiffness and displacement of the particle from trap center extracted a probable bond rupture force of ≈ 18 pN. The probability of rupture events with c-CPE functionalized silica particles increased by 50% compared to unfunctionalized particles. Additionally, rupture events were not observed when probing cells not expressing claudin with c-CPE coated particles. Overall, this work demonstrates that optical tweezers are invaluable tools to probe ligand-receptor interactions and their potential to study dynamic molecular events in drug-binding scenarios.
KW - C-CPE
KW - cell receptors
KW - claudin
KW - force spectroscopy
KW - ligand
KW - optical tweezers
UR - http://www.scopus.com/inward/record.url?scp=85097076266&partnerID=8YFLogxK
U2 - 10.3389/fbioe.2020.598459
DO - 10.3389/fbioe.2020.598459
M3 - Article
AN - SCOPUS:85097076266
VL - 8
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
M1 - 598459
ER -