Details
| Original language | English |
|---|---|
| Article number | 010903 |
| Pages (from-to) | 010903 |
| Journal | APL bioengineering |
| Volume | 6 |
| Issue number | 1 |
| Early online date | 2 Mar 2022 |
| Publication status | Published - Mar 2022 |
Abstract
Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell-cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the "black box" of living cells.
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In: APL bioengineering, Vol. 6, No. 1, 010903, 03.2022, p. 010903.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Principles for the design of multicellular engineered living systems
AU - Aydin, Onur
AU - Passaro, Austin P
AU - Raman, Ritu
AU - Spellicy, Samantha E
AU - Weinberg, Robert P
AU - Kamm, Roger D
AU - Sample, Matthew
AU - Truskey, George A
AU - Zartman, Jeremiah
AU - Dar, Roy D
AU - Palacios, Sebastian
AU - Wang, Jason
AU - Tordoff, Jesse
AU - Montserrat, Nuria
AU - Bashir, Rashid
AU - Saif, M Taher A
AU - Weiss, Ron
N1 - Funding Information: This work was supported by the National Science Foundation Science and Technology Center for Emergent Behaviors of Integrated Cellular Systems, Grant No. 0939511. The authors would like to thank Dr. Shun Zhang, Massachusetts Institute for Technology, for kindly providing the schematic and immunostaining images presented in Fig. 4(c) and Dr. Kristina Davis, Center for Research
PY - 2022/3
Y1 - 2022/3
N2 - Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell-cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the "black box" of living cells.
AB - Remarkable progress in bioengineering over the past two decades has enabled the formulation of fundamental design principles for a variety of medical and non-medical applications. These advancements have laid the foundation for building multicellular engineered living systems (M-CELS) from biological parts, forming functional modules integrated into living machines. These cognizant design principles for living systems encompass novel genetic circuit manipulation, self-assembly, cell-cell/matrix communication, and artificial tissues/organs enabled through systems biology, bioinformatics, computational biology, genetic engineering, and microfluidics. Here, we introduce design principles and a blueprint for forward production of robust and standardized M-CELS, which may undergo variable reiterations through the classic design-build-test-debug cycle. This Review provides practical and theoretical frameworks to forward-design, control, and optimize novel M-CELS. Potential applications include biopharmaceuticals, bioreactor factories, biofuels, environmental bioremediation, cellular computing, biohybrid digital technology, and experimental investigations into mechanisms of multicellular organisms normally hidden inside the "black box" of living cells.
UR - http://www.scopus.com/inward/record.url?scp=85126231130&partnerID=8YFLogxK
U2 - 10.1063/5.0076635
DO - 10.1063/5.0076635
M3 - Article
C2 - 35274072
VL - 6
SP - 010903
JO - APL bioengineering
JF - APL bioengineering
SN - 2473-2877
IS - 1
M1 - 010903
ER -