Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems

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Original languageEnglish
Title of host publicationCell-Based Microarrays
PublisherSpringer Verlag
Pages131-145
Number of pages15
ISBN (electronic)978-1-4939-7792-5
ISBN (print)978-1-4939-7791-8
Publication statusPublished - 10 Apr 2018

Publication series

NameMethods in Molecular Biology
Volume1771
ISSN (Print)1064-3745

Abstract

The deposition of living cells on microarray surfaces can be used to create physiologically relevant architecture in vitro. Such living cell microarrays enable the reconstruction of biological processes outside the body in a miniaturized format and have many advantages over traditional cell culture. The present protocol offers an option for the preparation and analysis of living primary and stem cell-based microarrays utilizing the standard microarray equipment (contact-free piezoelectric nanoprinter, microarray scanner), as well as microscopy. To produce living cell microarrays, we applied two kinds of mesenchymal stem cells (MSCs) isolated from umbilical cord and adipose tissue, as well as human umbilical vein endothelial cells (HUVECs) as model cells. We used live imaging microscopy for the online monitoring of cell spots in total size, staining of viable cells with Calcein acetoxymethyl ester (Calcein-AM) and treatment of MSCs with differentiation media to analyze the proliferation, viability, and differentiation potential of printed cells. This way, the general applicability of the established living cell-based microarray production was demonstrated.

Keywords

    Endothelial cells, Living cell microarrays, Mesenchymal stem cells, Microarray technology, Online monitoring microscopy, Piezoelectric nanoprinting, Primary human cells

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Cite this

Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems. / Jonczyk, Rebecca; Scheper, Thomas; Stahl, Frank.
Cell-Based Microarrays. Springer Verlag, 2018. p. 131-145 (Methods in Molecular Biology; Vol. 1771).

Research output: Chapter in book/report/conference proceedingContribution to book/anthologyResearchpeer review

Jonczyk, R, Scheper, T & Stahl, F 2018, Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems. in Cell-Based Microarrays. Methods in Molecular Biology, vol. 1771, Springer Verlag, pp. 131-145. https://doi.org/10.1007/978-1-4939-7792-5_11
Jonczyk, R., Scheper, T., & Stahl, F. (2018). Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems. In Cell-Based Microarrays (pp. 131-145). (Methods in Molecular Biology; Vol. 1771). Springer Verlag. https://doi.org/10.1007/978-1-4939-7792-5_11
Jonczyk R, Scheper T, Stahl F. Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems. In Cell-Based Microarrays. Springer Verlag. 2018. p. 131-145. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-7792-5_11
Jonczyk, Rebecca ; Scheper, Thomas ; Stahl, Frank. / Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems. Cell-Based Microarrays. Springer Verlag, 2018. pp. 131-145 (Methods in Molecular Biology).
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