Details
| Original language | English |
|---|---|
| Article number | 150 |
| Number of pages | 17 |
| Journal | Stem Cell Research and Therapy |
| Volume | 15 |
| Publication status | Published - 23 May 2024 |
Abstract
Mesenchymal stem/stromal cells (MSCs) are not only capable of self-renewal, trans-differentiation, homing to damaged tissue sites and immunomodulation by secretion of trophic factors but are also easy to isolate and expand. Because of these characteristics, they are used in numerous clinical trials for cell therapy including immune and neurological disorders, diabetes, bone and cartilage diseases and myocardial infarction. However, not all trials have successful outcomes, due to unfavourable microenvironmental factors and the heterogenous nature of MSCs. Therefore, genetic manipulation of MSCs can increase their prospect. Currently, most studies focus on single transfection with one gene. Even though the introduction of more than one gene increases the complexity, it also increases the effectivity as different mechanism are triggered, leading to a synergistic effect. In this review we focus on the methodology and efficiency of co-transfection, as well as the opportunities and pitfalls of these genetically engineered cells for therapy. Graphical abstract: (Figure presented.)
Keywords
- CRISPR/Cas9, Electroporation, Genetic modification, Lipofection, Mesenchymal stem cells, Viral transfection
ASJC Scopus subject areas
- Medicine(all)
- Medicine (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
Sustainable Development Goals
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Stem Cell Research and Therapy, Vol. 15, 150, 23.05.2024.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Possibilities and efficiency of MSC co-transfection for gene therapy
AU - Christoffers, Sina
AU - Seiler, Lisa
AU - Wiebe, Elena
AU - Blume, Cornelia
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024/5/23
Y1 - 2024/5/23
N2 - Mesenchymal stem/stromal cells (MSCs) are not only capable of self-renewal, trans-differentiation, homing to damaged tissue sites and immunomodulation by secretion of trophic factors but are also easy to isolate and expand. Because of these characteristics, they are used in numerous clinical trials for cell therapy including immune and neurological disorders, diabetes, bone and cartilage diseases and myocardial infarction. However, not all trials have successful outcomes, due to unfavourable microenvironmental factors and the heterogenous nature of MSCs. Therefore, genetic manipulation of MSCs can increase their prospect. Currently, most studies focus on single transfection with one gene. Even though the introduction of more than one gene increases the complexity, it also increases the effectivity as different mechanism are triggered, leading to a synergistic effect. In this review we focus on the methodology and efficiency of co-transfection, as well as the opportunities and pitfalls of these genetically engineered cells for therapy. Graphical abstract: (Figure presented.)
AB - Mesenchymal stem/stromal cells (MSCs) are not only capable of self-renewal, trans-differentiation, homing to damaged tissue sites and immunomodulation by secretion of trophic factors but are also easy to isolate and expand. Because of these characteristics, they are used in numerous clinical trials for cell therapy including immune and neurological disorders, diabetes, bone and cartilage diseases and myocardial infarction. However, not all trials have successful outcomes, due to unfavourable microenvironmental factors and the heterogenous nature of MSCs. Therefore, genetic manipulation of MSCs can increase their prospect. Currently, most studies focus on single transfection with one gene. Even though the introduction of more than one gene increases the complexity, it also increases the effectivity as different mechanism are triggered, leading to a synergistic effect. In this review we focus on the methodology and efficiency of co-transfection, as well as the opportunities and pitfalls of these genetically engineered cells for therapy. Graphical abstract: (Figure presented.)
KW - CRISPR/Cas9
KW - Electroporation
KW - Genetic modification
KW - Lipofection
KW - Mesenchymal stem cells
KW - Viral transfection
UR - http://www.scopus.com/inward/record.url?scp=85194130052&partnerID=8YFLogxK
U2 - 10.1186/s13287-024-03757-6
DO - 10.1186/s13287-024-03757-6
M3 - Review article
C2 - 38783353
AN - SCOPUS:85194130052
VL - 15
JO - Stem Cell Research and Therapy
JF - Stem Cell Research and Therapy
SN - 1757-6512
M1 - 150
ER -