Details
| Original language | English |
|---|---|
| Article number | 1617 |
| Journal | Foods |
| Volume | 9 |
| Issue number | 11 |
| Publication status | Published - 6 Nov 2020 |
Abstract
Secondary plant metabolites, e.g., polyphenols, are widely known as health-improving compounds that occur in natural functional foods such as pomegranates. While extracts generated from these fruits inhibit oxidative stress, the allocation of these effects to the different subgroups of substances, e.g., anthocyanins, "copigments" (polyphenols without anthocyanins), or polymeric compounds, is still unknown. Therefore, in the present study, polyphenols from pomegranate juice were extracted and separated into an anthocyanin and copigment fraction using adsorptive membrane chromatography. Phenolic compounds were determined by high performance liquid chromatography with photodiode array (HPLC-PDA) detection and HPLC-PDA electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI-MS/MS), while the free radical scavenging activity of the pomegranate XAD‑7 extract and its fractions was evaluated by the Trolox equivalent antioxidant capacity (TEAC) assay and electron spin resonance (ESR) spectroscopy. Compared to juice, the total phenolic content and free radical scavenging potential was significantly higher in the pomegranate XAD-7 extract and its fractions. In comparison to the anthocyanin and copigment fraction, pomegranate XAD-7 extract showed the highest radical scavenging activity against galvinoxyl and DPPH radicals. Moreover, the enriched XAD-7 extract and its fractions were able to protect human hepatocellular HepG2 cells against oxidative stress induced by hydrogen peroxide. Overall, these results indicated that anthocyanins and copigments act together in reducing oxidative stress.
Keywords
- Adsorptive membrane chromatography, Antioxidant, Cell culture, Electron spin resonance spectroscopy, HepG2, HPLC, Pomegranate
ASJC Scopus subject areas
- Social Sciences(all)
- Health(social science)
- Agricultural and Biological Sciences(all)
- Food Science
- Health Professions(all)
- Health Professions (miscellaneous)
- Agricultural and Biological Sciences(all)
- Plant Science
- Immunology and Microbiology(all)
- Microbiology
Sustainable Development Goals
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In: Foods, Vol. 9, No. 11, 1617, 06.11.2020.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Pomegranate (Punica granatum L.) extract and Its anthocyanin and copigment fractions
T2 - Free radical scavenging activity and influence on cellular oxidative stress
AU - Kostka, Tina
AU - Ostberg-Potthoff, Johanna Josefine
AU - Briviba, Karlis
AU - Matsugo, Seiichi
AU - Winterhalter, Peter
AU - Esatbeyoglu, Tuba
N1 - Funding Information: Funding: The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover.
PY - 2020/11/6
Y1 - 2020/11/6
N2 - Secondary plant metabolites, e.g., polyphenols, are widely known as health-improving compounds that occur in natural functional foods such as pomegranates. While extracts generated from these fruits inhibit oxidative stress, the allocation of these effects to the different subgroups of substances, e.g., anthocyanins, "copigments" (polyphenols without anthocyanins), or polymeric compounds, is still unknown. Therefore, in the present study, polyphenols from pomegranate juice were extracted and separated into an anthocyanin and copigment fraction using adsorptive membrane chromatography. Phenolic compounds were determined by high performance liquid chromatography with photodiode array (HPLC-PDA) detection and HPLC-PDA electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI-MS/MS), while the free radical scavenging activity of the pomegranate XAD‑7 extract and its fractions was evaluated by the Trolox equivalent antioxidant capacity (TEAC) assay and electron spin resonance (ESR) spectroscopy. Compared to juice, the total phenolic content and free radical scavenging potential was significantly higher in the pomegranate XAD-7 extract and its fractions. In comparison to the anthocyanin and copigment fraction, pomegranate XAD-7 extract showed the highest radical scavenging activity against galvinoxyl and DPPH radicals. Moreover, the enriched XAD-7 extract and its fractions were able to protect human hepatocellular HepG2 cells against oxidative stress induced by hydrogen peroxide. Overall, these results indicated that anthocyanins and copigments act together in reducing oxidative stress.
AB - Secondary plant metabolites, e.g., polyphenols, are widely known as health-improving compounds that occur in natural functional foods such as pomegranates. While extracts generated from these fruits inhibit oxidative stress, the allocation of these effects to the different subgroups of substances, e.g., anthocyanins, "copigments" (polyphenols without anthocyanins), or polymeric compounds, is still unknown. Therefore, in the present study, polyphenols from pomegranate juice were extracted and separated into an anthocyanin and copigment fraction using adsorptive membrane chromatography. Phenolic compounds were determined by high performance liquid chromatography with photodiode array (HPLC-PDA) detection and HPLC-PDA electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI-MS/MS), while the free radical scavenging activity of the pomegranate XAD‑7 extract and its fractions was evaluated by the Trolox equivalent antioxidant capacity (TEAC) assay and electron spin resonance (ESR) spectroscopy. Compared to juice, the total phenolic content and free radical scavenging potential was significantly higher in the pomegranate XAD-7 extract and its fractions. In comparison to the anthocyanin and copigment fraction, pomegranate XAD-7 extract showed the highest radical scavenging activity against galvinoxyl and DPPH radicals. Moreover, the enriched XAD-7 extract and its fractions were able to protect human hepatocellular HepG2 cells against oxidative stress induced by hydrogen peroxide. Overall, these results indicated that anthocyanins and copigments act together in reducing oxidative stress.
KW - Adsorptive membrane chromatography
KW - Antioxidant
KW - Cell culture
KW - Electron spin resonance spectroscopy
KW - HepG2
KW - HPLC
KW - Pomegranate
UR - http://www.scopus.com/inward/record.url?scp=85109360272&partnerID=8YFLogxK
U2 - 10.3390/foods9111617
DO - 10.3390/foods9111617
M3 - Article
C2 - 33172172
VL - 9
JO - Foods
JF - Foods
IS - 11
M1 - 1617
ER -