Details
| Original language | English |
|---|---|
| Pages (from-to) | 631-9 |
| Number of pages | 9 |
| Journal | Plant Journal |
| Volume | 32 |
| Issue number | 5 |
| Publication status | Published - Dec 2002 |
Abstract
In light-, but not in dark-grown spinach seedlings, the mRNAs for the nuclear-encoded photosystem I subunits D, F and L are associated with polyribosomes and this association is prevented by the application of 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea (DCMU), an inhibitor of the photosynthetic electron transport. To identify the cis-elements which are responsible for this regulation, we generated a series of chimeric PsaD constructs and tested them in transgenic tobacco. The spinach PsaD 5'-untranslated region is sufficient to confer light- and photosynthesis-dependent polyribosome association onto the uidA reporter gene, while the tobacco PsaD 5'-untranslated region directs constitutive polyribosome association. These results are discussed with regard to signals from photosynthetic electron flow which control processes in the cytoplasm.
Keywords
- Base Sequence, Darkness, Electron Transport, Light, Molecular Sequence Data, Photosynthesis, Photosystem I Protein Complex/genetics, Plants, Genetically Modified, Polyribosomes/metabolism, RNA, Messenger/genetics, Spinacia oleracea/genetics, Time Factors, Tobacco/genetics
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In: Plant Journal, Vol. 32, No. 5, 12.2002, p. 631-9.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Polyribosome loading of spinach mRNAs for photosystem I subunits is controlled by photosynthetic electron transport
AU - Sherameti, Irena
AU - Nakamura, Masayaki
AU - Yamamoto, Yoshiharu Y
AU - Pfannschmidt, Thomas
AU - Obokata, Junichi
AU - Oelmüller, Ralf
PY - 2002/12
Y1 - 2002/12
N2 - In light-, but not in dark-grown spinach seedlings, the mRNAs for the nuclear-encoded photosystem I subunits D, F and L are associated with polyribosomes and this association is prevented by the application of 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea (DCMU), an inhibitor of the photosynthetic electron transport. To identify the cis-elements which are responsible for this regulation, we generated a series of chimeric PsaD constructs and tested them in transgenic tobacco. The spinach PsaD 5'-untranslated region is sufficient to confer light- and photosynthesis-dependent polyribosome association onto the uidA reporter gene, while the tobacco PsaD 5'-untranslated region directs constitutive polyribosome association. These results are discussed with regard to signals from photosynthetic electron flow which control processes in the cytoplasm.
AB - In light-, but not in dark-grown spinach seedlings, the mRNAs for the nuclear-encoded photosystem I subunits D, F and L are associated with polyribosomes and this association is prevented by the application of 3-(3',4'-dichlorophenyl)-1,1'-dimethyl urea (DCMU), an inhibitor of the photosynthetic electron transport. To identify the cis-elements which are responsible for this regulation, we generated a series of chimeric PsaD constructs and tested them in transgenic tobacco. The spinach PsaD 5'-untranslated region is sufficient to confer light- and photosynthesis-dependent polyribosome association onto the uidA reporter gene, while the tobacco PsaD 5'-untranslated region directs constitutive polyribosome association. These results are discussed with regard to signals from photosynthetic electron flow which control processes in the cytoplasm.
KW - Base Sequence
KW - Darkness
KW - Electron Transport
KW - Light
KW - Molecular Sequence Data
KW - Photosynthesis
KW - Photosystem I Protein Complex/genetics
KW - Plants, Genetically Modified
KW - Polyribosomes/metabolism
KW - RNA, Messenger/genetics
KW - Spinacia oleracea/genetics
KW - Time Factors
KW - Tobacco/genetics
U2 - 10.1046/j.1365-313x.2002.01452.x
DO - 10.1046/j.1365-313x.2002.01452.x
M3 - Article
C2 - 12472681
VL - 32
SP - 631
EP - 639
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 5
ER -