Details
| Original language | English |
|---|---|
| Pages (from-to) | 231-240 |
| Number of pages | 10 |
| Journal | Plant Physiology and Biochemistry |
| Volume | 96 |
| Early online date | 10 Aug 2015 |
| Publication status | Published - 1 Nov 2015 |
Abstract
Polyamines (PA) are catabolised by two groups of amine oxidases, the copper-binding amine oxidases (CuAOs) and the FAD-binding polyamine oxidases (PAOs). Previously, we have shown that CuAO1 is involved in ABA associated growth responses and ABA- and PA-mediated rapid nitric oxide (NO) production. Here we report the differential regulation of expression of POLYAMINE OXIDASE2 of Arabidopsis (AtPAO2) in interaction with ABA, nitrate and ammonium. Without ABA treatment germination, cotyledon growth and fresh weight of pao2 knockdown mutants as well as PAO2OX over-expressor plants were comparable to those of the wild type (WT) plants irrespective of the N source. In the presence of ABA, in pao2 mutants cotyledon growth and fresh weights were more sensitive to inhibition by ABA while PAO2OX over-expressor plants showed a rather similar response to WT. When NO3- was the only N source primary root lengths and lateral root numbers were lower in pao2 mutants both without and with exogenous ABA. PAO2OX showed enhanced primary and lateral root growth in media with NO3- or NH4+. Vigorous root growth of PAO2OX and the hypersensitivity of pao2 mutants to ABA suggest a positive function of AtPAO2 in root growth. ABA-induced NO production in pao2 mutants was lower indicating a potential contributory function of AtPAO2 in NO-mediated effects on root growth.
Keywords
- Abscisic acid, Ammonia, Nitrate, NO: nitric oxide, POLYAMINE OXIDASE2, Root development
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant Physiology and Biochemistry, Vol. 96, 01.11.2015, p. 231-240.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - POLYAMINE OXIDASE2 of Arabidopsis contributes to ABA mediated plant developmental processes
AU - Wimalasekera, Rinukshi
AU - Schaarschmidt, Frank
AU - Angelini, Riccardo
AU - Cona, Alessandra
AU - Tavladoraki, Parasklevi
AU - Scherer, Günther F.E.
N1 - Funding Information: R.W. was supported by a grant from the Leibniz Universität Hannover (“Postdoc Programm zur Wiedereingliederung des weiblichen wissenschaftlichen Nachwuchses”). Publisher Copyright: © 2015 Elsevier Masson SAS.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Polyamines (PA) are catabolised by two groups of amine oxidases, the copper-binding amine oxidases (CuAOs) and the FAD-binding polyamine oxidases (PAOs). Previously, we have shown that CuAO1 is involved in ABA associated growth responses and ABA- and PA-mediated rapid nitric oxide (NO) production. Here we report the differential regulation of expression of POLYAMINE OXIDASE2 of Arabidopsis (AtPAO2) in interaction with ABA, nitrate and ammonium. Without ABA treatment germination, cotyledon growth and fresh weight of pao2 knockdown mutants as well as PAO2OX over-expressor plants were comparable to those of the wild type (WT) plants irrespective of the N source. In the presence of ABA, in pao2 mutants cotyledon growth and fresh weights were more sensitive to inhibition by ABA while PAO2OX over-expressor plants showed a rather similar response to WT. When NO3- was the only N source primary root lengths and lateral root numbers were lower in pao2 mutants both without and with exogenous ABA. PAO2OX showed enhanced primary and lateral root growth in media with NO3- or NH4+. Vigorous root growth of PAO2OX and the hypersensitivity of pao2 mutants to ABA suggest a positive function of AtPAO2 in root growth. ABA-induced NO production in pao2 mutants was lower indicating a potential contributory function of AtPAO2 in NO-mediated effects on root growth.
AB - Polyamines (PA) are catabolised by two groups of amine oxidases, the copper-binding amine oxidases (CuAOs) and the FAD-binding polyamine oxidases (PAOs). Previously, we have shown that CuAO1 is involved in ABA associated growth responses and ABA- and PA-mediated rapid nitric oxide (NO) production. Here we report the differential regulation of expression of POLYAMINE OXIDASE2 of Arabidopsis (AtPAO2) in interaction with ABA, nitrate and ammonium. Without ABA treatment germination, cotyledon growth and fresh weight of pao2 knockdown mutants as well as PAO2OX over-expressor plants were comparable to those of the wild type (WT) plants irrespective of the N source. In the presence of ABA, in pao2 mutants cotyledon growth and fresh weights were more sensitive to inhibition by ABA while PAO2OX over-expressor plants showed a rather similar response to WT. When NO3- was the only N source primary root lengths and lateral root numbers were lower in pao2 mutants both without and with exogenous ABA. PAO2OX showed enhanced primary and lateral root growth in media with NO3- or NH4+. Vigorous root growth of PAO2OX and the hypersensitivity of pao2 mutants to ABA suggest a positive function of AtPAO2 in root growth. ABA-induced NO production in pao2 mutants was lower indicating a potential contributory function of AtPAO2 in NO-mediated effects on root growth.
KW - Abscisic acid
KW - Ammonia
KW - Nitrate
KW - NO: nitric oxide
KW - POLYAMINE OXIDASE2
KW - Root development
UR - http://www.scopus.com/inward/record.url?scp=84939626752&partnerID=8YFLogxK
U2 - 10.1016/j.plaphy.2015.08.003
DO - 10.1016/j.plaphy.2015.08.003
M3 - Article
C2 - 26310141
AN - SCOPUS:84939626752
VL - 96
SP - 231
EP - 240
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
SN - 0981-9428
ER -