Details
| Original language | English |
|---|---|
| Title of host publication | Plasmonics in Biology and Medicine VI |
| Publication status | Published - 18 Feb 2009 |
| Event | Plasmonics in Biology and Medicine VI - San Jose, CA, United States Duration: 26 Jan 2009 → 27 Jan 2009 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 7192 |
| ISSN (Print) | 1605-7422 |
Abstract
Investigation on the interaction of small particles, e.g. gold nanoparticles with light is a current field of high interest. As light can be absorbed, enhanced or scattered by the nanoparticles a wide variety of possible applications become possible. If the electrons of such a nanoparticles oscillate with the incident light, plasmon resonances occur. Provided that these particles are brought very close to a cell, the cell membrane gets perforated due to the laser induced effect. We investigate nanoparticle mediated laser perforation as an alternative technique for cell transfection. By using weakly focussed femtosecond laser pulses, 150 nm gold particles were stimulated to perforate the cell membrane. Through the perforated area of the membrane macromolecules e.g. DNA are able to enter the cell. By this technique GFSHR-17 rat cells were successfully transfected with GFP vector and the dependence on laser parameters and concentration were studied. Even after 48 hours after manipulation the transfected cells show no indications of apoptosis or necrosis. This technique allows the transfection of cells by opto-perforation without the need of tight focusing conditions and single cell targeting- opening the way for a wide field of applications.
Keywords
- Cell manipulation, Nanoparticles, Plasmon resonance, Ultrashort laser pulses
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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Plasmonics in Biology and Medicine VI. 2009. 71920U (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7192).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Plasmonic perforation of living cells using ultrashort laser pulses and gold nanoparticles
AU - Schomaker, Markus
AU - Baumgart, Judith
AU - Ngezahayo, Anaclet
AU - Bullerdiek, Jörn
AU - Nolte, Ingo
AU - Murua Escobar, Hugo
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2009/2/18
Y1 - 2009/2/18
N2 - Investigation on the interaction of small particles, e.g. gold nanoparticles with light is a current field of high interest. As light can be absorbed, enhanced or scattered by the nanoparticles a wide variety of possible applications become possible. If the electrons of such a nanoparticles oscillate with the incident light, plasmon resonances occur. Provided that these particles are brought very close to a cell, the cell membrane gets perforated due to the laser induced effect. We investigate nanoparticle mediated laser perforation as an alternative technique for cell transfection. By using weakly focussed femtosecond laser pulses, 150 nm gold particles were stimulated to perforate the cell membrane. Through the perforated area of the membrane macromolecules e.g. DNA are able to enter the cell. By this technique GFSHR-17 rat cells were successfully transfected with GFP vector and the dependence on laser parameters and concentration were studied. Even after 48 hours after manipulation the transfected cells show no indications of apoptosis or necrosis. This technique allows the transfection of cells by opto-perforation without the need of tight focusing conditions and single cell targeting- opening the way for a wide field of applications.
AB - Investigation on the interaction of small particles, e.g. gold nanoparticles with light is a current field of high interest. As light can be absorbed, enhanced or scattered by the nanoparticles a wide variety of possible applications become possible. If the electrons of such a nanoparticles oscillate with the incident light, plasmon resonances occur. Provided that these particles are brought very close to a cell, the cell membrane gets perforated due to the laser induced effect. We investigate nanoparticle mediated laser perforation as an alternative technique for cell transfection. By using weakly focussed femtosecond laser pulses, 150 nm gold particles were stimulated to perforate the cell membrane. Through the perforated area of the membrane macromolecules e.g. DNA are able to enter the cell. By this technique GFSHR-17 rat cells were successfully transfected with GFP vector and the dependence on laser parameters and concentration were studied. Even after 48 hours after manipulation the transfected cells show no indications of apoptosis or necrosis. This technique allows the transfection of cells by opto-perforation without the need of tight focusing conditions and single cell targeting- opening the way for a wide field of applications.
KW - Cell manipulation
KW - Nanoparticles
KW - Plasmon resonance
KW - Ultrashort laser pulses
UR - http://www.scopus.com/inward/record.url?scp=66649103000&partnerID=8YFLogxK
U2 - 10.1117/12.809316
DO - 10.1117/12.809316
M3 - Conference contribution
AN - SCOPUS:66649103000
SN - 9780819474384
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Plasmonics in Biology and Medicine VI
T2 - Plasmonics in Biology and Medicine VI
Y2 - 26 January 2009 through 27 January 2009
ER -