Details
| Original language | English |
|---|---|
| Title of host publication | Plasmonics in Biology and Medicine XII |
| Editors | Joseph R. Lakowicz, Tuan Vo-Dinh |
| Publisher | SPIE |
| ISBN (electronic) | 9781628414301 |
| Publication status | Published - 11 Mar 2015 |
| Externally published | Yes |
| Event | Plasmonics in Biology and Medicine XII - San Francisco, United States Duration: 7 Feb 2015 → 8 Feb 2015 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 9340 |
| ISSN (Print) | 1605-7422 |
Abstract
Modulation of the cell membrane permeability by the plasmonic interaction of gold nanoparticles and short laser pulses for cell manipulation or destruction has been the objective of several recent studies. Gold nanoparticles in close vicinity to the cellular membrane are irradiated to evoke a nanoscale membrane perforation, enabling extracellular molecules to enter the cell. However, besides several basic studies no real translation from proof of concept experiments to routine usage of this approach was achieved so far. In order to provide a reproducible and easy-to-use platform for gold nanoparticle mediated (GNOME) laser manipulation, we established an automated and encased laser setup. We demonstrate its feasibility for high-throughput cell manipulation. In particular, protein delivery into canine cancer cells is shown. The biofunctional modification of cells was investigated using the caspase 3 protein, which represents a central effector molecule in the apoptotic signaling cascade. An efficient and temporally well-defined induction of apoptosis was observed with an early onset 2 h after protein delivery by GNOME laser manipulation. Besides protein delivery, modulation of gene function using GNOME laser transfection of antisense molecules was demonstrated, showing the potential of this technique for basic science and screening purposes. Concluding, we established GNOME laser manipulation of cells as a routine method, which can be utilized reliably for the efficient delivery of biomolecules. Its intrinsic features, being low impairment of the cell viability, high delivery efficiency and universal applicability, render this method well suited for a large variety of biomedical application.
Keywords
- Apoptosis, Laser transfection, Plasmonics, Protein delivery
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Materials Science(all)
- Biomaterials
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Sustainable Development Goals
Cite this
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- BibTeX
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Plasmonics in Biology and Medicine XII. ed. / Joseph R. Lakowicz; Tuan Vo-Dinh. SPIE, 2015. 93400Q-1 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9340).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Plasmonic cell manipulation for biomedical and screening applications
AU - Heinemann, Dag
AU - Schomaker, Markus
AU - Kalies, Stefan
AU - Sinram, Merve
AU - Heeger, Patrick
AU - Escobar, Hugo Murua
AU - Meyer, Heiko
AU - Ripken, Tammo
PY - 2015/3/11
Y1 - 2015/3/11
N2 - Modulation of the cell membrane permeability by the plasmonic interaction of gold nanoparticles and short laser pulses for cell manipulation or destruction has been the objective of several recent studies. Gold nanoparticles in close vicinity to the cellular membrane are irradiated to evoke a nanoscale membrane perforation, enabling extracellular molecules to enter the cell. However, besides several basic studies no real translation from proof of concept experiments to routine usage of this approach was achieved so far. In order to provide a reproducible and easy-to-use platform for gold nanoparticle mediated (GNOME) laser manipulation, we established an automated and encased laser setup. We demonstrate its feasibility for high-throughput cell manipulation. In particular, protein delivery into canine cancer cells is shown. The biofunctional modification of cells was investigated using the caspase 3 protein, which represents a central effector molecule in the apoptotic signaling cascade. An efficient and temporally well-defined induction of apoptosis was observed with an early onset 2 h after protein delivery by GNOME laser manipulation. Besides protein delivery, modulation of gene function using GNOME laser transfection of antisense molecules was demonstrated, showing the potential of this technique for basic science and screening purposes. Concluding, we established GNOME laser manipulation of cells as a routine method, which can be utilized reliably for the efficient delivery of biomolecules. Its intrinsic features, being low impairment of the cell viability, high delivery efficiency and universal applicability, render this method well suited for a large variety of biomedical application.
AB - Modulation of the cell membrane permeability by the plasmonic interaction of gold nanoparticles and short laser pulses for cell manipulation or destruction has been the objective of several recent studies. Gold nanoparticles in close vicinity to the cellular membrane are irradiated to evoke a nanoscale membrane perforation, enabling extracellular molecules to enter the cell. However, besides several basic studies no real translation from proof of concept experiments to routine usage of this approach was achieved so far. In order to provide a reproducible and easy-to-use platform for gold nanoparticle mediated (GNOME) laser manipulation, we established an automated and encased laser setup. We demonstrate its feasibility for high-throughput cell manipulation. In particular, protein delivery into canine cancer cells is shown. The biofunctional modification of cells was investigated using the caspase 3 protein, which represents a central effector molecule in the apoptotic signaling cascade. An efficient and temporally well-defined induction of apoptosis was observed with an early onset 2 h after protein delivery by GNOME laser manipulation. Besides protein delivery, modulation of gene function using GNOME laser transfection of antisense molecules was demonstrated, showing the potential of this technique for basic science and screening purposes. Concluding, we established GNOME laser manipulation of cells as a routine method, which can be utilized reliably for the efficient delivery of biomolecules. Its intrinsic features, being low impairment of the cell viability, high delivery efficiency and universal applicability, render this method well suited for a large variety of biomedical application.
KW - Apoptosis
KW - Laser transfection
KW - Plasmonics
KW - Protein delivery
UR - http://www.scopus.com/inward/record.url?scp=84928580730&partnerID=8YFLogxK
U2 - 10.1117/12.2077604
DO - 10.1117/12.2077604
M3 - Conference contribution
AN - SCOPUS:84928580730
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Plasmonics in Biology and Medicine XII
A2 - Lakowicz, Joseph R.
A2 - Vo-Dinh, Tuan
PB - SPIE
T2 - Plasmonics in Biology and Medicine XII
Y2 - 7 February 2015 through 8 February 2015
ER -