Details
| Original language | English |
|---|---|
| Pages (from-to) | 524-527 |
| Number of pages | 4 |
| Journal | Soil Biology and Biochemistry |
| Volume | 42 |
| Issue number | 3 |
| Publication status | Published - Mar 2010 |
Abstract
Direct plant uptake of organic nitrogen (N) is often studied using the dual-labeling approach (15N + 13C or 15N + 14C). However, the method might be hampered by uptake of labeled inorganic carbon (C) produced by mineralization of labeled organic compounds. Here we report the results from a triple labeling experiment (15N + 13C + 14C) investigating whether root uptake of labeled inorganic C can bias the results obtained in studies of organic N uptake using dual-labeled amino acids (15N, 13C). In a rhizosphere tube experiment we investigated 13C and 14C uptake by maize either supplied with labeled glycine or CO32 -, but found no differences in uptake rates between these C-sources. The uptake of inorganic C to the shoot tissue was higher for maize grown in full light compared to shading, which indicates a passive uptake of inorganic C with water. We conclude that uptake of inorganic C produced by mineralization of amino acids can significantly bias the interpretations of organic N uptake studies using dual-labeling.
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Soil Science
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In: Soil Biology and Biochemistry, Vol. 42, No. 3, 03.2010, p. 524-527.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Plant uptake of dual-labeled organic N biased by inorganic C uptake
T2 - Results of a triple labeling study
AU - Rasmussen, Jim
AU - Sauheitl, Leopold
AU - Eriksen, Jørgen
AU - Kuzyakov, Yakov
N1 - Funding information: The present work was supported by the European Science Foundation (ESF) through the program “Nitrogen in Europe: Assessment of current problems and future solutions”.
PY - 2010/3
Y1 - 2010/3
N2 - Direct plant uptake of organic nitrogen (N) is often studied using the dual-labeling approach (15N + 13C or 15N + 14C). However, the method might be hampered by uptake of labeled inorganic carbon (C) produced by mineralization of labeled organic compounds. Here we report the results from a triple labeling experiment (15N + 13C + 14C) investigating whether root uptake of labeled inorganic C can bias the results obtained in studies of organic N uptake using dual-labeled amino acids (15N, 13C). In a rhizosphere tube experiment we investigated 13C and 14C uptake by maize either supplied with labeled glycine or CO32 -, but found no differences in uptake rates between these C-sources. The uptake of inorganic C to the shoot tissue was higher for maize grown in full light compared to shading, which indicates a passive uptake of inorganic C with water. We conclude that uptake of inorganic C produced by mineralization of amino acids can significantly bias the interpretations of organic N uptake studies using dual-labeling.
AB - Direct plant uptake of organic nitrogen (N) is often studied using the dual-labeling approach (15N + 13C or 15N + 14C). However, the method might be hampered by uptake of labeled inorganic carbon (C) produced by mineralization of labeled organic compounds. Here we report the results from a triple labeling experiment (15N + 13C + 14C) investigating whether root uptake of labeled inorganic C can bias the results obtained in studies of organic N uptake using dual-labeled amino acids (15N, 13C). In a rhizosphere tube experiment we investigated 13C and 14C uptake by maize either supplied with labeled glycine or CO32 -, but found no differences in uptake rates between these C-sources. The uptake of inorganic C to the shoot tissue was higher for maize grown in full light compared to shading, which indicates a passive uptake of inorganic C with water. We conclude that uptake of inorganic C produced by mineralization of amino acids can significantly bias the interpretations of organic N uptake studies using dual-labeling.
UR - http://www.scopus.com/inward/record.url?scp=74149087812&partnerID=8YFLogxK
U2 - 10.1016/j.soilbio.2009.11.032
DO - 10.1016/j.soilbio.2009.11.032
M3 - Article
AN - SCOPUS:74149087812
VL - 42
SP - 524
EP - 527
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
IS - 3
ER -